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Molecular Markers for Fungal Detection and Identification, 2nd Edition
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Molecular Markers for Fungal Detection and Identification, 2nd Edition

A special issue of Journal of Fungi (ISSN 2309-608X). This special issue belongs to the section "Fungal Genomics, Genetics and Molecular Biology".

Deadline for manuscript submissions: closed (31 August 2023) | Viewed by 2772

Special Issue Editor

Dr. Manuela Oliveira

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Guest Editor
Cooperativa de Ensino Superior, Politécnico e Universitário, Gandra, Portugal
Interests: microbiology; microbial forensics; botany; botany forensic; molecular markers; genetics
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The latest estimates indicate a total of 1.5 million fungal species, only half of them described, ubiquitously distributed through almost all the ecological niches from the biosphere. These microorganisms are widely known for their recycling capacity of organic matter and their significant role in pathogenesis, either in humans, animals, or crops.

Since its development in the early 1980s, molecular techniques based on polymerase chain reactions have revolutionized the molecular diagnosis of fungi, allowing a rapid, unambiguous detection and identification of these organisms. Among these markers are Short Tandem Repeat (STR), Single Nucleotide Polymorphism (SNP), small Insertions or Deletions (InDels), and DNA barcoding, each presenting their own advantages and disadvantages.

As such, the present Special Issue on "Molecular markers for fungal detection and identification" aims to perform a literature review welcoming both research and review manuscripts of the most recent advances on this topic.

Dr. Manuela Oliveira
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Journal of Fungi is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • fungi
  • molecular marker
  • detection
  • identification
  • human diseases
  • agricultural diseases (animals and crops)
  • short tandem repeat (STR)
  • single nucleotide polymorphism (SNP)
  • small insertions or deletions (InDels)
  • DNA barcoding

Published Papers (2 papers)

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Research

9 pages, 591 KiB  
Communication
How Different Molecular Markers Estimate the Diversity of European Species of the Ganoderma Genus
by Peter Pristas, Terezia Beck, Lea Nosalova, Svetlana Gaperova and Jan Gaper
J. Fungi 2023, 9(10), 1023; https://doi.org/10.3390/jof9101023 - 17 Oct 2023
Cited by 1 | Viewed by 1156
Abstract
Based on published anatomical-morphological and ecological characteristics and phylogenetic evidence, six species of the Ganoderma genus are known to occur in Europe, namely, G. applanatum (Pers.) Pat., G. adspersum (Schulzer) Donk, G. pfeifferi Bres., G. resinaceum Boud., G. carnosum Pat., and G. lucidum [...] Read more.
Based on published anatomical-morphological and ecological characteristics and phylogenetic evidence, six species of the Ganoderma genus are known to occur in Europe, namely, G. applanatum (Pers.) Pat., G. adspersum (Schulzer) Donk, G. pfeifferi Bres., G. resinaceum Boud., G. carnosum Pat., and G. lucidum (Curtis) P. Karst. Molecular markers (DNA sequences of selected genes or intergenic spacers) revolutionized our view of fungal variability. Every one of the four most frequently used molecular markers (ITS (internal transcribed spacer) and partial sequences of LSU (rRNA large subunit), tef1-α (translation elongation factor 1-alpha), and Rpb2 (RNA polymerase II second largest subunit)) provides a different view on the variability of European species of the Ganoderma genus. Both the lowest intraspecies variability and the best species differentiation (interspecies divergence) were observed for the LSU marker, and based on our data, this marker could be recommended for identification and species delineation in European Ganoderma spp. The sequences of the most frequently used ITS marker were unable to discriminate between G. lucidum and G. carnosum, and in general, this species pair showed the lowest interspecies divergence using all markers tested. Surprisingly, up to now, hidden variability has been detected in several Ganoderma spp., indicating the existence of possible cryptic taxa within the European Ganoderma morphospecies. Full article
(This article belongs to the Special Issue Molecular Markers for Fungal Detection and Identification, 2nd Edition)
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7 pages, 1189 KiB  
Communication
Comparison of β-1-3-D-Glucan and Candida Mannan Biomarker Assays with Serological Tests for the Diagnosis of Candidemia
by Christopher P. Eades, Ahmed Rafezzan Bin Ahmed Bakri, Jeffrey C. Y. Lau, Caroline B. Moore, Lilyann Novak-Frazer, Malcolm D. Richardson and Riina Rautemaa-Richardson
J. Fungi 2023, 9(8), 813; https://doi.org/10.3390/jof9080813 - 31 Jul 2023
Cited by 1 | Viewed by 1304
Abstract
Invasive candidiasis, including bloodstream infection (candidemia), encompasses the most severe forms of Candida infection. Several species-specific and non-specific serological assays are commercially available to aid in diagnosis. This study compared the performance of five such biomarker assays. Serum samples from 14 patients with [...] Read more.
Invasive candidiasis, including bloodstream infection (candidemia), encompasses the most severe forms of Candida infection. Several species-specific and non-specific serological assays are commercially available to aid in diagnosis. This study compared the performance of five such biomarker assays. Serum samples from 14 patients with proven or probable invasive candidiasis, and from 10 control patients, were included in the analysis. A total of 50 serum samples were tested using C. albicans germ tube antibody (CAGTA) assay (Vircell), C. albicans IgM, C. albicans IgG and Candida mannan assays (Dynamiker Biotechnology). Among these samples, the β-1-3-D-glucan (BDG) assay (Fungitell), a laboratory standard for the diagnosis of invasive candidiasis, was positive in 20 (40%), intermediate in five (10%) and negative in 25 (50%). In cases of proven or probable candidemia, the sensitivity and specificity of the BDG assay was 86% and 80%, respectively; the Candida mannan assay, 14% and 86%; the CAGTA test, 57% and 60%; the C. albicans IgM assay, 71% and 60%; and C. albicans IgG assay 29% and 90%. In 4/8 (50%) cases with multiple serum samples, C. albicans IgM was positive sooner than BDG. Thus, when used as a rule-out test for invasive candidiasis, our data suggest that the C. albicans IgM assay may assist antifungal stewardship (over serum BDG). Full article
(This article belongs to the Special Issue Molecular Markers for Fungal Detection and Identification, 2nd Edition)
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