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Stem Cell Research for Future Therapies and In Vitro Disease Modelling

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Biology".

Deadline for manuscript submissions: closed (30 September 2023) | Viewed by 2502

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Guest Editor
Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden
Interests: regenerative medicine; stem cells; organoids; cell replacement therapies; neurodegeneration; neural stem cells; cardiomyocytes; retinal disorders
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Special Issue Information

Dear Colleagues,

In recent years, stem cell therapy has emerged as a very promising and advanced scientific research topic that is currently undergoing its first clinical trials. This growing interest coincides with industrial partners aims to scale up technology and capacity and provide cell replacement therapies to patients suffering from chronic disorders.

This Special Issue focuses on recent discoveries that may contribute to the areas of basic and molecular stem cell research, in vitro disease modelling, molecular stem cell biology, exosomes, translational stem cell research, and stem cell therapies. Recent advances in cell reprogramming and genome editing technologies have created new possibilities for the development of novel in vitro models to study human diseases. More recently, organoids have emerged as a powerful tool to better study early developmental biology and as a potential source for future cell therapies.

Cell-replacement therapies are entering a new phase of growth, and novel discoveries for molecular characterization, cell differentiation, expansion, purification, raw materials, and delivery under GMP conditions are critical for moving stem cell products from bench to bedside.

We invite you to contribute to this Special Issue and communicate original articles and reviews on new advances in stem cell research for future therapies and in vitro disease modelling.

Dr. J. Carlos Villaescusa
Guest Editor

Manuscript Submission Information

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Published Papers (1 paper)

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19 pages, 5613 KiB  
Article
Derivation of Human Extraembryonic Mesoderm-like Cells from Primitive Endoderm
by Karin Farkas and Elisabetta Ferretti
Int. J. Mol. Sci. 2023, 24(14), 11366; https://doi.org/10.3390/ijms241411366 - 12 Jul 2023
Viewed by 2301
Abstract
In vitro modeling of human peri-gastrulation development is a valuable tool for understanding embryogenetic mechanisms. The extraembryonic mesoderm (ExM) is crucial in supporting embryonic development by forming tissues such as the yolk sac, allantois, and chorionic villi. However, the origin of human ExM [...] Read more.
In vitro modeling of human peri-gastrulation development is a valuable tool for understanding embryogenetic mechanisms. The extraembryonic mesoderm (ExM) is crucial in supporting embryonic development by forming tissues such as the yolk sac, allantois, and chorionic villi. However, the origin of human ExM remains only partially understood. While evidence suggests a primitive endoderm (PrE) origin based on morphological findings, current in vitro models use epiblast-like cells. To address this gap, we developed a protocol to generate ExM-like cells from PrE-like cell line called naïve extraembryonic endoderm (nEnd). We identified the ExM-like cells by specific markers (LUM and ANXA1). Moreover, these in vitro-produced ExM cells displayed angiogenic potential on a soft matrix, mirroring their physiological role in vasculogenesis. By integrating single-cell RNA sequencing (scRNAseq) data, we found that the ExM-like cells clustered with the LUM/ANXA1-rich cell populations of the gastrulating embryo, indicating similarity between in vitro and ex utero cell populations. This study confirms the derivation of ExM from PrE and establishes a cell culture system that can be utilized to investigate ExM during human peri-gastrulation development, both in monolayer cultures and more complex models. Full article
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