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Advances in Plant Breeding and Resistance

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Plant Sciences".

Deadline for manuscript submissions: 30 April 2024 | Viewed by 4518

Special Issue Editor


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Guest Editor
Department of Plant Resouces and Environment, Jeju National University, Jeju 63243, Republic of Korea
Interests: phenomics; precision agriculture; plant breeding; smart farm; germplasm enhancement
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

We are pleased to announce a Special Issue in the International Journal of Molecular Sciences focusing on cutting-edge research and advancements in the field of plant breeding and resistance, specifically highlighting the revolutionary methodologies of phenomics, genomics, and gene editing.

This Special Issue aims to provide a comprehensive platform for researchers, scientists, and experts from around the world to share their groundbreaking research, innovative methodologies, and insightful findings. The Special Issue will encompass a wide range of topics related to phenomics, genomics, and gene editing as applied in plant breeding and resistance.

The scope of this Special Issue includes, but is not limited to, the following topics:

  1. Phenomic approaches for high-throughput plant phenotyping;
  2. Genomic tools and technologies for plant genome analysis and characterization;
  3. Gene editing techniques (CRISPR/Cas9, TALENs, etc.) and their applications in plant improvement and disease resistance;
  4. Molecular mechanisms underlying plant resistance to biotic and abiotic stresses;
  5. Genetic variation and diversity studies in crop species for improved resilience;
  6. Integrative omics approaches in elucidating plant-pathogen interactions;
  7. Computational modeling and artificial intelligence in phenomics and genomics research.

Dr. Yong Suk Chung
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • plant phenomics
  • high-throughput phenotyping
  • plant cells
  • plant genomics
  • plant genetics
  • gene editing
  • crop breeding
  • CRISPR/Cas9
  • abiotic stress
  • plant resistance
  • saline and alkali resistance
  • drought tolerance
  • genomics
  • resistance

Published Papers (4 papers)

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Research

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22 pages, 5854 KiB  
Article
Revealing the Complete Bispecific Phosphatase Genes (DUSPs) across the Genome and Investigating the Expression Patterns of GH_A11G3500 Resistance against Verticillium wilt
by Yahui Deng, Xiaojuan Deng, Jieyin Zhao, Shuo Ning, Aixing Gu, Quanjia Chen and Yanying Qu
Int. J. Mol. Sci. 2024, 25(8), 4500; https://doi.org/10.3390/ijms25084500 - 19 Apr 2024
Viewed by 197
Abstract
DUSPs, a diverse group of protein phosphatases, play a pivotal role in orchestrating cellular growth and development through intricate signaling pathways. Notably, they actively participate in the MAPK pathway, which governs crucial aspects of plant physiology, including growth regulation, disease resistance, pest resistance, [...] Read more.
DUSPs, a diverse group of protein phosphatases, play a pivotal role in orchestrating cellular growth and development through intricate signaling pathways. Notably, they actively participate in the MAPK pathway, which governs crucial aspects of plant physiology, including growth regulation, disease resistance, pest resistance, and stress response. DUSP is a key enzyme, and it is the enzyme that limits the rate of cell metabolism. At present, complete understanding of the DUSP gene family in cotton and its specific roles in resistance to Verticillium wilt (VW) remains elusive. To address this knowledge gap, we conducted a comprehensive identification and analysis of four key cotton species: Gossypium arboreum, Gossypium barbadense, Gossypium hirsutum, and Gossypium raimondii. The results revealed the identification of a total of 120 DUSP genes in the four cotton varieties, which were categorized into six subgroups and randomly distributed at both ends of 26 chromosomes, predominantly localized within the nucleus. Our analysis demonstrated that closely related DUSP genes exhibited similarities in terms of the conserved motif composition and gene structure. A promoter analysis performed on the GhDUSP gene promoter revealed the presence of several cis-acting elements, which are associated with abiotic and biotic stress responses, as well as hormone signaling. A tissue expression pattern analysis demonstrated significant variations in GhDUSP gene expression under different stress conditions, with roots exhibiting the highest levels, followed by stems and leaves. In terms of tissue-specific detection, petals, leaves, stems, stamens, and receptacles exhibited higher expression levels of the GhDUSP gene. The gene expression analysis results for GhDUSPs under stress suggest that DUSP genes may have a crucial role in the cotton response to stress in cotton. Through Virus-Induced Gene Silencing (VIGS) experiments, the silencing of the target gene significantly reduced the resistance efficiency of disease-resistant varieties against Verticillium wilt (VW). Consequently, we conclude that GH_A11G3500-mediated bispecific phosphorylated genes may serve as key regulators in the resistance of G. hirsutum to Verticillium wilt (VW). This study presents a comprehensive structure designed to provide an in-depth understanding of the potential biological functions of cotton, providing a strong foundation for further research into molecular breeding and resistance to plant pathogens. Full article
(This article belongs to the Special Issue Advances in Plant Breeding and Resistance)
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13 pages, 1965 KiB  
Article
Patch Track Software for Measuring Kinematic Phenotypes of Arabidopsis Roots Demonstrated on Auxin Transport Mutants
by Ashley R. Henry, Nathan D. Miller and Edgar P. Spalding
Int. J. Mol. Sci. 2023, 24(22), 16475; https://doi.org/10.3390/ijms242216475 - 18 Nov 2023
Viewed by 765
Abstract
Plant roots elongate when cells produced in the apical meristem enter a transient period of rapid expansion. To measure the dynamic process of root cell expansion in the elongation zone, we captured digital images of growing Arabidopsis roots with horizontal microscopes and analyzed [...] Read more.
Plant roots elongate when cells produced in the apical meristem enter a transient period of rapid expansion. To measure the dynamic process of root cell expansion in the elongation zone, we captured digital images of growing Arabidopsis roots with horizontal microscopes and analyzed them with a custom image analysis program (PatchTrack) designed to track the growth-driven displacement of many closely spaced image patches. Fitting a flexible logistics equation to patch velocities plotted versus position along the root axis produced the length of the elongation zone (mm), peak relative elemental growth rate (% h−1), the axial position of the peak (mm from the tip), and average root elongation rate (mm h−1). For a wild-type root, the average values of these kinematic traits were 0.52 mm, 23.7% h−1, 0.35 mm, and 0.1 mm h−1, respectively. We used the platform to determine the kinematic phenotypes of auxin transport mutants. The results support a model in which the PIN2 auxin transporter creates an area of expansion-suppressing, supraoptimal auxin concentration that ends 0.1 mm from the quiescent center (QC), and that ABCB4 and ABCB19 auxin transporters maintain expansion-limiting suboptimal auxin levels beginning approximately 0.5 mm from the QC. This study shows that PatchTrack can quantify dynamic root phenotypes in kinematic terms. Full article
(This article belongs to the Special Issue Advances in Plant Breeding and Resistance)
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16 pages, 3255 KiB  
Article
Resistant and Susceptible Pinus thunbergii ParL. Show Highly Divergent Patterns of Differentially Expressed Genes during the Process of Infection by Bursaphelenchus xylophilus
by Tingyu Sun, Mati Ur Rahman, Xiaoqin Wu and Jianren Ye
Int. J. Mol. Sci. 2023, 24(18), 14376; https://doi.org/10.3390/ijms241814376 - 21 Sep 2023
Viewed by 865
Abstract
Pine wilt disease (PWD) is a devastating disease that threatens pine forests worldwide, and breeding resistant pines is an important management strategy used to reduce its impact. A batch of resistant seeds of P. thunbergii was introduced from Japan. Based on the resistant [...] Read more.
Pine wilt disease (PWD) is a devastating disease that threatens pine forests worldwide, and breeding resistant pines is an important management strategy used to reduce its impact. A batch of resistant seeds of P. thunbergii was introduced from Japan. Based on the resistant materials, we obtained somatic plants through somatic embryogenesis. In this study, we performed transcriptome analysis to further understand the defense response of resistant somatic plants of P. thunbergii to PWD. The results showed that, after pine wood nematode (PWN) infection, resistant P. thunbergii stimulated more differential expression genes (DEGs) and involved more regulatory pathways than did susceptible P. thunbergii. For the first time, the alpha-linolenic acid metabolism and linoleic acid metabolism were intensively observed in pines resisting PWN infection. The related genes disease resistance protein RPS2 (SUMM2) and pathogenesis-related genes (PR1), as well as reactive oxygen species (ROS)-related genes were significantly up-expressed in order to contribute to protection against PWN inoculation in P. thunbergii. In addition, the diterpenoid biosynthesis pathway was significantly enriched only in resistant P. thunbergii. These findings provided valuable genetic information for future breeding of resistant conifers, and could contribute to the development of new diagnostic tools for early screening of resistant pine seedlings based on specific PWN-tolerance-related markers. Full article
(This article belongs to the Special Issue Advances in Plant Breeding and Resistance)
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Review

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13 pages, 1147 KiB  
Review
CRISPR Variants for Gene Editing in Plants: Biosafety Risks and Future Directions
by Ali Movahedi, Soheila Aghaei-Dargiri, Hongyan Li, Qiang Zhuge and Weibo Sun
Int. J. Mol. Sci. 2023, 24(22), 16241; https://doi.org/10.3390/ijms242216241 - 13 Nov 2023
Cited by 1 | Viewed by 2000
Abstract
The CRISPR genome editing technology is a crucial tool for enabling revolutionary advancements in plant genetic improvement. This review shows the latest developments in CRISPR/Cas9 genome editing system variants, discussing their benefits and limitations for plant improvement. While this technology presents immense opportunities [...] Read more.
The CRISPR genome editing technology is a crucial tool for enabling revolutionary advancements in plant genetic improvement. This review shows the latest developments in CRISPR/Cas9 genome editing system variants, discussing their benefits and limitations for plant improvement. While this technology presents immense opportunities for plant breeding, it also raises serious biosafety concerns that require careful consideration, including potential off-target effects and the unintended transfer of modified genes to other organisms. This paper highlights strategies to mitigate biosafety risks and explores innovative plant gene editing detection methods. Our review investigates the international biosafety guidelines for gene-edited crops, analyzing their broad implications for agricultural and biotechnology research and advancement. We hope to provide illuminating and refined perspectives for industry practitioners and policymakers by evaluating CRISPR genome enhancement in plants. Full article
(This article belongs to the Special Issue Advances in Plant Breeding and Resistance)
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