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Molecular Insights into Infectious Diseases

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Pathology, Diagnostics, and Therapeutics".

Deadline for manuscript submissions: closed (31 January 2024) | Viewed by 953

Special Issue Editor


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Guest Editor
Department of Microbiology, The University of Hong Kong, Hong Kong, China
Interests: infectious diseases; virus; diagnostics; PCR; sequencing; genomics; phylogenetic analysis; molecular epidemiology

Special Issue Information

Dear Colleagues,

Infectious diseases are caused by pathogens, including viruses, bacteria, fungi or parasites. The diseases can be transmitted, directly or indirectly, from one person to another. A recent pandemic caused by SARS-CoV-2, which is one of the largest pandemics in recorded history, led to significant morbidity, mortality and socioeconomic disruption globally. Diagnosis, epidemiological study, prevention and treatment play critical roles in combating infectious disease outbreaks. Therefore, the development of rapid molecular diagnostic tests allows for the early detection of causative agents, which is important for timely patient management and infection control. Furthermore, research on molecular epidemiology provides a way to track the spread and evolution of pathogens. In addition, a detailed understanding of molecular mechanisms of pathogenesis may guide the identification of novel antimicrobials for treatment or the development of vaccines for disease prevention. This Special Issue welcomes original research articles or reviews in these areas.

Dr. Cyril Chik-Yan Yip
Guest Editor

Manuscript Submission Information

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Keywords

  • infectious disease
  • microorganism
  • pathogen
  • infection
  • molecular diagnosis
  • molecular epidemiology
  • pathogenesis
  • antimicrobial
  • vaccine

Published Papers (1 paper)

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Research

11 pages, 1638 KiB  
Article
Development and Evaluation of an In-House Real-Time RT-PCR Targeting nsp10 Gene for SARS-CoV-2 Detection
by Cyril Chik-Yan Yip, Jane Hau-Ching Poon, Kit-Hang Leung, Wan-Mui Chan, Jonathan Daniel Ip, Allen Wing-Ho Chu, Vincent Chi-Chung Cheng, Kwok-Yung Yuen and Kelvin Kai-Wang To
Int. J. Mol. Sci. 2024, 25(6), 3552; https://doi.org/10.3390/ijms25063552 - 21 Mar 2024
Viewed by 677
Abstract
The emergence of SARS-CoV-2 mutations poses significant challenges to diagnostic tests, as these mutations can reduce the sensitivity of commonly used RT-PCR assays. Therefore, there is a need to design diagnostic assays with multiple targets to enhance sensitivity. In this study, we identified [...] Read more.
The emergence of SARS-CoV-2 mutations poses significant challenges to diagnostic tests, as these mutations can reduce the sensitivity of commonly used RT-PCR assays. Therefore, there is a need to design diagnostic assays with multiple targets to enhance sensitivity. In this study, we identified a novel diagnostic target, the nsp10 gene, using nanopore sequencing. Firstly, we determined the analytical sensitivity and specificity of our COVID-19-nsp10 assay. The COVID-19-nsp10 assay had a limit of detection of 74 copies/mL (95% confidence interval: 48–299 copies/mL) and did not show cross-reactivity with other respiratory viruses. Next, we determined the diagnostic performance of the COVID-19-nsp10 assay using 261 respiratory specimens, including 147 SARS-CoV-2-positive specimens belonging to the ancestral strain and Alpha, Beta, Gamma, Delta, Mu, Eta, Kappa, Theta and Omicron lineages. Using a LightMix E-gene RT-PCR assay as the reference method, the diagnostic sensitivity and specificity of the COVID-19-nsp10 assay were found to be 100%. The median Cp values for the LightMix E-gene RT-PCR and our COVID-19-nsp10 RT-PCR were 22.48 (range: 12.95–36.60) and 25.94 (range 16.37–36.87), respectively. The Cp values of the COVID-19-nsp10 RT-PCR assay correlated well with those of the LightMix E-gene RT-PCR assay (Spearman’s ρ = 0.968; p < 0.0001). In conclusion, nsp10 is a suitable target for a SARS-CoV-2 RT-PCR assay. Full article
(This article belongs to the Special Issue Molecular Insights into Infectious Diseases)
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