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Studying Wildlife Diseases—the Application of Molecular Research

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Microbiology".

Deadline for manuscript submissions: closed (29 February 2024) | Viewed by 1094

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Guest Editor
Veterinary Faculty, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia
Interests: wildlife diseases; conservation; parasitology; epidemiology
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Wildlife health is a constantly growing field that encourages an interdisciplinary approach and continuously attracts scientists. Wildlife is important for several reasons (as a valuable public asset, heritage for future generations, and as an essential part of ecosystems providing balance and normal functioning), but also due to an increasing human population and urbanization, wildlife has become a more and more important player in the wildlife–human–livestock interface. Although the role of wildlife in the maintenance and transmission of pathogens is still not fully understood, they can potentially pose a threat to human and domestic animal health, not to mention that certain wildlife diseases can significantly threaten the conservation of endangered species. The introduction of molecular tools into studies of wildlife diseases has elucidated and sometimes revised our understanding of the maintenance, transmission, pathogenesis, and treatment of wildlife diseases. The rapid development of novel molecular techniques offers new possibilities and new knowledge in studies of wildlife pathogens.

Dr. Dean Konjevic
Guest Editor

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Keywords

  • wildlife
  • diseases
  • pathogens
  • zoonosis
  • molecular tools

Published Papers (1 paper)

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Research

18 pages, 4500 KiB  
Article
Molecular Detection and Genetic Diversity of Cytomegaloviruses and Lymphocryptoviruses in Free-Roaming and Captive African Green Monkeys (Chlorocebus sabaeus)
by Diana M. Mancuso, Kerry Gainor, Kerry M. Dore, Christa A. Gallagher, Amy Beierschmitt, Yashpal S. Malik and Souvik Ghosh
Int. J. Mol. Sci. 2024, 25(6), 3272; https://doi.org/10.3390/ijms25063272 - 14 Mar 2024
Viewed by 912
Abstract
To date, limited information is available on cytomegalovirus (CMV) and lymphocryptovirus (LCV) from Chlorocebus monkeys. We report here high detection rates of herpesviruses in free-roaming African green monkeys (AGMs, Chlorocebus sabaeus) (26.4%, 23/87) and in captive AGMs (75%, 3/4) with respiratory disease [...] Read more.
To date, limited information is available on cytomegalovirus (CMV) and lymphocryptovirus (LCV) from Chlorocebus monkeys. We report here high detection rates of herpesviruses in free-roaming African green monkeys (AGMs, Chlorocebus sabaeus) (26.4%, 23/87) and in captive AGMs (75%, 3/4) with respiratory disease on the Caribbean Island of St. Kitts. LCV (81.25%) was more prevalent than CMV (18.75%) in the AGMs. Applying a bigenic PCR approach (targeting DNA polymerase (DPOL) and glycoprotein B (gB) genes), long sequences were obtained from representative AGM CMV (KNA-SD6) and LCV (KNA-E4, -N6 and -R15) samples, and mixed LCV infections were identified in KNA-N6 and -R15. The nucleotide (nt) sequence (partial DPOL-intergenic region-partial gB) and partial DPOL- and gB-amino acid (aa) sequences of AGM CMV KNA-SD6 were closely related to Cytomegalovirus cercopithecinebeta5 isolates from grivet monkeys, whilst those of AGM LCV KNA-E4 and -N6 (and E4-like gB of KNA-R15) were more closely related to cognate sequences of erythrocebus patas LCV1 from patas monkey than other LCVs, corroborating the concept of cospeciation in the evolution of CMV/LCV. On the other hand, the partial DPOL aa sequence of KNA-R15, and additional gB sequences (N6-gB-2 and R15-gB-2) from samples KNA-N6 and -R15 (respectively) appeared to be distinct from those of Old World monkey LCVs, indicating LCV evolutionary patterns that were not synchronous with those of host species. The present study is the first to report the molecular prevalence and genetic diversity of CMV/LCV from free-roaming/wild and captive AGMs, and is the first report on analysis of CMV nt/deduced aa sequences from AGMs and LCV gB sequences from Chlorocebus monkeys. Full article
(This article belongs to the Special Issue Studying Wildlife Diseases—the Application of Molecular Research)
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