Submit to IJMS Review for IJMS Propose a Special Issue

Journal Menu

Journal Browser

Soybean Molecular Breeding and Genetics 2.0

Special Issue Editors
Special Issue Information
Keywords
Published Papers

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Plant Sciences".

Deadline for manuscript submissions: closed (16 December 2022) | Viewed by 7054

Share This Special Issue

Special Issue Editor

Prof. Dr. Yinghui Li

E-Mail Website
Guest Editor

The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI)/Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Interests: soybean; genetics; gonomics; breeding; selection; gene discovery; evolution
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Soybean is the major source of plant protein and oil in the world. Molecular breeding has become the core technology for driving the development of global soybean production. A better understanding of the underlying molecular mechanisms of important traits and further improvement in soybean using new technologies such as CRISPR genome editing for higher yield, better nutritional quality and improved stress tolerance will play a key role in achieving green and sustainable agriculture.

We seek submissions of research articles and review articles on both basic research and technological advancements in soybean breeding and genetics. We welcome articles in the areas of gene and allele identification by QTL/association mapping, gene discovery and mechanism analysis, functional molecular markers and chip development, genome-wide selection, genetic engineering, gene editing, mutagenesis and molecular pyramid breeding, among other related areas. Papers submitted to this Special Issue must report high-novelty results and/or new plausible and testable models for the integrative analysis of the different approaches applied to soybean breeding.

Prof. Dr. Yinghui Li
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

favorable allele identification
gene discovery
genetic analysis
molecular breeding

Published Papers (4 papers)

Download All Papers

Order results

Result details

Show export options Show export options

Select all

Export citation of selected articles as:

Research

16 pages, 2740 KiB

Open AccessArticle

Identifications of QTLs and Candidate Genes Associated with Pseudomonas syringae Responses in Cultivated Soybean (Glycine max) and Wild Soybean (Glycine soja)

by Jinhui Wang, Haojie Feng, Xiaoke Jia, Shengnan Ma, Chao Ma, Yue Wang, Siyang Pan, Qingshan Chen, Dawei Xin and Chunyan Liu

Int. J. Mol. Sci. 2023, 24(5), 4618; https://doi.org/10.3390/ijms24054618 - 27 Feb 2023

Cited by 3 | Viewed by 1809

Abstract

Soybeans (Glycine max) are a key food crop, serving as a valuable source of both oil and plant-derived protein. Pseudomonas syringae pv. glycinea (Psg) is among the most aggressive and prevalent pathogens affecting soybean production, causing a form of bacterial spot disease that impacts soybean leaves and thereby reduces crop yields. In this study, 310 natural soybean varieties were screened for Psg resistance and susceptibility. The identified susceptible and resistant varieties were then used for linkage mapping, BSA-seq, and whole genome sequencing (WGS) analyses aimed at identifying key QTLs associated with Psg responses. Candidate Psg-related genes were further confirmed through WGS and qPCR analyses. Candidate gene haplotype analyses were used to explore the associations between haplotypes and soybean Psg resistance. In addition, landrace and wild soybean plants were found to exhibit a higher degree of Psg resistance as compared to cultivated soybean varieties. In total, 10 QTLs were identified using chromosome segment substitution lines derived from Suinong14 (cultivated soybean) and ZYD00006 (wild soybean). Glyma.10g230200 was found to be induced in response to Psg, with the Glyma.10g230200 haplotype corresponding to soybean disease resistance. The QTLs identified herein can be leveraged to guide the marker-assisted breeding of soybean cultivars that exhibit partial resistance to Psg. Moreover, further functional and molecular studies of Glyma.10g230200 have the potential to offer insight into the mechanistic basis for soybean Psg resistance. Full article

(This article belongs to the Special Issue Soybean Molecular Breeding and Genetics 2.0)

► Show Figures

Figure 1

16 pages, 3523 KiB

Open AccessArticle

CRISPR/Cas9-Mediated Multiple Knockouts in Abscisic Acid Receptor Genes Reduced the Sensitivity to ABA during Soybean Seed Germination

by Zhaohan Zhang, Wanpeng Wang, Shahid Ali, Xiao Luo and Linan Xie

Int. J. Mol. Sci. 2022, 23(24), 16173; https://doi.org/10.3390/ijms232416173 - 18 Dec 2022

Cited by 3 | Viewed by 1771

Abstract

Abscisic acid (ABA) is an important plant hormone that regulates numerous functions in plant growth, development, and stress responses. Several proteins regulate the ABA signal transduction mechanism in response to environmental stress. Among them, the PYR1/PYL/RCAR family act as ABA receptors. This study used the CRISPR/Cas9 gene-editing system with a single gRNA to knock out three soybean PYL genes: GmPYL17, GmPYL18, and GmPYL19. The gRNA may efficiently cause varying degrees of deletion of GmPYL17, GmPYL18, and GmPYL19 gene target sequences, according to the genotyping results of T0 plants. A subset of induced alleles was successfully transferred to progeny. In the T2 generation, we obtained double and triple mutant genotypes. At the seed germination stage, CRISPR/Cas9-created GmPYL gene knockout mutants, particularly gmpyl17/19 double mutants, are less susceptible to ABA than the wild type. RNA-Seq was used to investigate the differentially expressed genes related to the ABA response from germinated seedlings under diverse treatments using three biological replicates. The gmpyl17/19-1 double mutant was less susceptible to ABA during seed germination, and mutant plant height and branch number were higher than the wild type. Under ABA stress, the GO enrichment analysis showed that certain positive germination regulators were activated, which reduced ABA sensitivity and enhanced seed germination. This research gives a theoretical basis for a better understanding of the ABA signaling pathway and the participation of the key component at their molecular level, which helps enhance soybean abiotic stress tolerance. Furthermore, this research will aid breeders in regulating and improving soybean production and quality under various stress conditions. Full article

(This article belongs to the Special Issue Soybean Molecular Breeding and Genetics 2.0)

► Show Figures

Figure 1

12 pages, 2484 KiB

Open AccessArticle

Genome-Wide Comparison of Structural Variations and Transposon Alterations in Soybean Cultivars Induced by Spaceflight

by Hangxia Jin, Xujun Fu, Xiaomin Yu, Longming Zhu, Qinghua Yang and Fengjie Yuan

Int. J. Mol. Sci. 2022, 23(22), 13721; https://doi.org/10.3390/ijms232213721 - 8 Nov 2022

Cited by 3 | Viewed by 1221

Abstract

Space mutation causes genetic and phenotypic changes in biological materials. Transposon activation is an adaptive mechanism for organisms to cope with changes in the external environment, such as space mutation. Although transposon alterations have been widely reported in diverse plant species, few studies have assessed the global transposon alterations in plants exposed to the space environment. In this study, for the first time, the effects of transposon alterations in soybean caused by space mutation were considered. A new vegetable soybean variety, ‘Zhexian 9’ (Z9), derived from space mutation treatment of ‘Taiwan 75’ (T75), was genetically analyzed. Comparative analyses of these two soybean genomes uncovered surprising structural differences, especially with respect to translocation breakends, deletions, and inversions. In total, 12,028 structural variations (SVs) and 29,063 transposable elements (TEs) between T75 and Z9 were detected. In addition, 1336 potential genes were variable between T75 and Z9 in terms of SVs and TEs. These differential genes were enriched in functions such as defense response, cell wall-related processes, epigenetics, auxin metabolism and transport, signal transduction, and especially methylation, which implied that regulation of epigenetic mechanisms and TE activity are important in the space environment. These results are helpful for understanding the role of TEs in response to the space environment and provide a theoretical basis for the selection of wild plant materials suitable for space breeding. Full article

(This article belongs to the Special Issue Soybean Molecular Breeding and Genetics 2.0)

► Show Figures

Figure 1

8 pages, 1721 KiB

Open AccessArticle

Using Staphylococcus aureus Cas9 to Expand the Scope of Potential Gene Targets for Genome Editing in Soybean

by Yan Zhang, Yupeng Cai, Shi Sun, Tianfu Han, Li Chen and Wensheng Hou

Int. J. Mol. Sci. 2022, 23(21), 12789; https://doi.org/10.3390/ijms232112789 - 24 Oct 2022

Cited by 2 | Viewed by 1432

Abstract

The CRISPR (clustered regularly interspaced short palindromic repeat)/Cas (CRISPR-associated) is a revolutionary genome editing technology that has been used to achieve site-specific gene knock-out, large fragment deletion, or base editing in many plant species including soybean (Glycinemax). The Streptococcuspyogenes Cas9 (SpCas9) is widely used in plants at present, although there are some reports describing the application of CRISPR/Cpf1 in soybean. Therefore, the selection range of PAM (protospacer adjacent motif) sequences for soybean is currently limited to 5′-NGG-3′ (SpCas9) or 5′-TTTN-3′ (Cpf1), which in turn limits the number of genes that can be mutated. Another Cas9 enzyme from Staphylococcus aureus (SaCas9) recognizes the PAM sequence 5′-NNGRRT-3′ (where R represents A or G), which can provide a wider range of potential target sequences. In this study, we developed a CRISPR/SaCas9 system and used this tool to specifically induce targeted mutations at five target sites in the GmFT2a (Glyma.16G150700) and GmFT5a (Glyma.16G044100) genes in soybean hairy roots. We demonstrated that this tool can recognize the PAM sequences 5′-AAGGGT-3′, 5′-GGGGAT-3′, 5′-TTGAAT-3′, and 5′-TAGGGT-3′ in soybean, and it achieved mutation rates ranging from 34.5% to 73.3%. Our results show that we have established a highly efficient CRISPR/SaCas9 tool that is as suitable as SpCas9 for genome editing in soybean, and it will be useful for expanding the range of target sequences for genome editing. Full article

(This article belongs to the Special Issue Soybean Molecular Breeding and Genetics 2.0)

► Show Figures

Journal Menu

Journal Browser

Soybean Molecular Breeding and Genetics 2.0

Share This Special Issue

Special Issue Editor

Special Issue Information

Keywords

Published Papers (4 papers)

Research

Further Information

Guidelines

MDPI Initiatives

Follow MDPI