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State-of-the-Art Molecular Plant Sciences in France
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State-of-the-Art Molecular Plant Sciences in France

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Plant Sciences".

Deadline for manuscript submissions: closed (28 February 2023) | Viewed by 11262

Special Issue Editors

Dr. Willy Vincent Bienvenut

E-Mail Website
Collection Editor
IDEEV- GQE – Le Moulon, Université Paris-Saclay, INRAE, CNRS, AgroParisTech, 91190 Gif sur Yvette, France
Interests: proteomics; mass spectrometry; bioinformatics; metabolic labeling; stable isotope labeling; 15N labeling; protein half-life; protein turnover; protein synthesis rate; protein degradation rate; prediction tools; N-terminal acetylation; protein N-terminal modification; protein maturation
Prof. Dr. Guillaume Tcherkez

E-Mail Website
Collection Editor
1. Institut de Recherche en Horticulture et semences UMR1345 INRAE, Université d’Angers, L’institut Agro, Beaucouzé, France
2. Research School of Biology, Australian National University, Canberra, ACT 2601, Australia
Interests: plant physiology; ecophysiology; isotopes; enzyme mechanisms; photosynthesis and respiration; nutrients; metabolomics and fluxomics
Dr. Jeremy Lothier

E-Mail Website
Collection Editor
Institut de Recherche en Horticulture et semences UMR1345 INRAE, Université d’Angers, L’institut Agro, Beaucouzé, France
Interests: plant biochemistry; primary metabolism regulation

Special Issue Information

Dear Colleagues

This Topical Collection aims to provide a comprehensive overview of recent advances in plant molecular science by inviting contributions from researchers hosted by French institutes/laboratories (public or private) that consolidate our knowledge in this research field. Potential topics of plants science may include but are not limited to the following:

  • Biophysics, biochemistry, and molecular biology;
  • Cell biology;
  • Ecophysiology and physiology;
  • Omics sciences: genomics/epigenomics, proteomics, metabolomics, etc.;
  • Bioactive phytochemicals;
  • Phytopathology;
  • Pests and diseases;
  • Plant–microbe interactions;
  • Developmental biology;
  • Synthetic biology;
  • Computational biology;
  • Development of new technologies in plant sciences.

The submission of original research articles describing significant advances (regular articles or technical insights) or comprehensive reviews related to plant science in all the abovementioned areas is encouraged.

Dr. Willy Vincent Bienvenut
Prof. Dr. Guillaume Tcherkez
Dr. Jérémy Lothier
Collection Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Published Papers (6 papers)

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Research

13 pages, 2245 KiB  
Article
The Light-Controlled Release of 2-fluoro-l-fucose, an Inhibitor of the Root Cell Elongation, from a nitrobenzyl-caged Derivative
by Mathieu Carlier, Thomas Poisson, Jean-Claude Mollet, Patrice Lerouge, Cyrille Sabot and Arnaud Lehner
Int. J. Mol. Sci. 2023, 24(3), 2533; https://doi.org/10.3390/ijms24032533 - 28 Jan 2023
Viewed by 1755
Abstract
Glycan metabolic engineering is a powerful tool for studying the glycosylation in living plant cells. The use of modified monosaccharides such as deoxy or fluorine-containing glycosides has been reported as a powerful pharmacological approach for studying the carbohydrate metabolism. 1,3,4-tri-O-acetyl-2-fluoro-l [...] Read more.
Glycan metabolic engineering is a powerful tool for studying the glycosylation in living plant cells. The use of modified monosaccharides such as deoxy or fluorine-containing glycosides has been reported as a powerful pharmacological approach for studying the carbohydrate metabolism. 1,3,4-tri-O-acetyl-2-fluoro-l-fucose (2F-Fuc) is a potent inhibitor of the plant cell elongation. After feeding plant seedlings with 2F-Fuc, this monosaccharide derivative is deacetylated and converted by the endogenous metabolic machinery into the corresponding nucleotide-sugar, which then efficiently inhibits Golgi-localized fucosyltransferases. Among plant cell wall polymers, defects in the fucosylation of the pectic rhamnogalacturonan-II cause a decrease in RG-II dimerization, which in turn induce the arrest of the cell elongation. In order to perform the inhibition of the cell elongation process in a spatio-temporal manner, we synthesized a caged 3,4-di-O-acetyl-1-hydroxy-2-fluoro-l-fucose (1-OH-2F-Fuc) derivative carrying a photolabile ortho-nitrobenzyl alcohol function at the anomeric position: 3,4-di-O-acetyl-1-ortho-nitrobenzyl-2-fluoro-l-fucose (2F-Fuc-NB). The photorelease of the trapped 1-OH-2F-Fuc was performed under a 365 nm LED illumination. We demonstrated that the in planta elimination by photoexcitation of the photolabile group releases free 2F-Fuc in plant cells, which in turn inhibits in a dose-dependent manner and, reversibly, the root cell elongation. Full article
(This article belongs to the Special Issue State-of-the-Art Molecular Plant Sciences in France)
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15 pages, 1647 KiB  
Article
Impact of Rhamnolipids (RLs), Natural Defense Elicitors, on Shoot and Root Proteomes of Brassica napus by a Tandem Mass Tags (TMTs) Labeling Approach
by Elise Pierre, Paulo Marcelo, Antoine Croutte, Morgane Dauvé, Sophie Bouton, Sonia Rippa and Karine Pageau
Int. J. Mol. Sci. 2023, 24(3), 2390; https://doi.org/10.3390/ijms24032390 - 25 Jan 2023
Cited by 1 | Viewed by 1582
Abstract
The rapeseed crop is susceptible to many pathogens such as parasitic plants or fungi attacking aerial or root parts. Conventional plant protection products, used intensively in agriculture, have a negative impact on the environment as well as on human health. There is therefore [...] Read more.
The rapeseed crop is susceptible to many pathogens such as parasitic plants or fungi attacking aerial or root parts. Conventional plant protection products, used intensively in agriculture, have a negative impact on the environment as well as on human health. There is therefore a growing demand for the development of more planet-friendly alternative protection methods such as biocontrol compounds. Natural rhamnolipids (RLs) can be used as elicitors of plant defense mechanisms. These glycolipids, from bacteria secretome, are biodegradable, non-toxic and are known for their stimulating and protective effects, in particular on rapeseed against filamentous fungi. Characterizing the organ responsiveness to defense-stimulating compounds such as RLs is missing. This analysis is crucial in the frame of optimizing the effectiveness of RLs against various diseases. A Tandem Mass Tags (TMT) labeling of the proteins extracted from the shoots and roots of rapeseed has been performed and showed a differential pattern of protein abundance between them. Quantitative proteomic analysis highlighted the differential accumulation of parietal and cytoplasmic defense or stress proteins in response to RL treatments with a clear effect of the type of application (foliar spraying or root absorption). These results must be considered for further use of RLs to fight specific rapeseed pathogens. Full article
(This article belongs to the Special Issue State-of-the-Art Molecular Plant Sciences in France)
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21 pages, 2375 KiB  
Article
The Arabidopsis thaliana–Streptomyces Interaction Is Controlled by the Metabolic Status of the Holobiont
by Stéfanie Graindorge, Claire Villette, Sandrine Koechler, Chloé Groh, Sophie Comtet-Marre, Pierre Mercier, Romaric Magerand, Pierre Peyret, Dimitri Heintz, Hubert Schaller and Florence Arsène-Ploetze
Int. J. Mol. Sci. 2022, 23(21), 12952; https://doi.org/10.3390/ijms232112952 - 26 Oct 2022
Cited by 4 | Viewed by 1689
Abstract
How specific interactions between plant and pathogenic, commensal, or mutualistic microorganisms are mediated and how bacteria are selected by a plant are important questions to address. Here, an Arabidopsis thaliana mutant called chs5 partially deficient in the biogenesis of isoprenoid precursors was shown [...] Read more.
How specific interactions between plant and pathogenic, commensal, or mutualistic microorganisms are mediated and how bacteria are selected by a plant are important questions to address. Here, an Arabidopsis thaliana mutant called chs5 partially deficient in the biogenesis of isoprenoid precursors was shown to extend its metabolic remodeling to phenylpropanoids and lipids in addition to carotenoids, chlorophylls, and terpenoids. Such a metabolic profile was concomitant to increased colonization of the phyllosphere by the pathogenic strain Pseudomonas syringae pv. tomato DC3000. A thorough microbiome analysis by 16S sequencing revealed that Streptomyces had a reduced colonization potential in chs5. This study revealed that the bacteria–Arabidopsis interaction implies molecular processes impaired in the chs5 mutant. Interestingly, our results revealed that the metabolic status of A. thaliana was crucial for the specific recruitment of Streptomyces into the microbiota. More generally, this study highlights specific as well as complex molecular interactions that shape the plant microbiota. Full article
(This article belongs to the Special Issue State-of-the-Art Molecular Plant Sciences in France)
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15 pages, 1775 KiB  
Article
Experimental Evidence for Seed Metabolic Allometry in Barrel Medic (Medicago truncatula Gaertn.)
by Jean-Baptiste Domergue, Julie Lalande, Daniel Beucher, Pascale Satour, Cyril Abadie, Anis M. Limami and Guillaume Tcherkez
Int. J. Mol. Sci. 2022, 23(15), 8484; https://doi.org/10.3390/ijms23158484 - 30 Jul 2022
Viewed by 1523
Abstract
Seed size is often considered to be an important trait for seed quality, i.e., vigour and germination performance. It is believed that seed size reflects the quantity of reserve material and thus the C and N sources available for post-germinative processes. However, mechanisms [...] Read more.
Seed size is often considered to be an important trait for seed quality, i.e., vigour and germination performance. It is believed that seed size reflects the quantity of reserve material and thus the C and N sources available for post-germinative processes. However, mechanisms linking seed size and quality are poorly documented. In particular, specific metabolic changes when seed size varies are not well-known. To gain insight into this aspect, we examined seed size and composition across different accessions of barrel medic (Medicago truncatula Gaertn.) from the genetic core collection. We conducted multi-elemental analyses and isotope measurements, as well as exact mass GC–MS metabolomics. There was a systematic increase in N content (+0.17% N mg−1) and a decrease in H content (–0.14% H mg−1) with seed size, reflecting lower lipid and higher S-poor protein quantity. There was also a decrease in 2H natural abundance (δ2H), due to the lower prevalence of 2H-enriched lipid hydrogen atoms that underwent isotopic exchange with water during seed development. Metabolomics showed that seed size correlates with free amino acid and hexoses content, and anticorrelates with amino acid degradation products, disaccharides, malic acid and free fatty acids. All accessions followed the same trend, with insignificant differences in metabolic properties between them. Our results show that there is no general, proportional increase in metabolite pools with seed size. Seed size appears to be determined by metabolic balance (between sugar and amino acid degradation vs. utilisation for storage), which is in turn likely determined by phloem source metabolite delivery during seed development. Full article
(This article belongs to the Special Issue State-of-the-Art Molecular Plant Sciences in France)
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19 pages, 2774 KiB  
Article
Leafamine®, a Free Amino Acid-Rich Biostimulant, Promotes Growth Performance of Deficit-Irrigated Lettuce
by Marthe Malécange, Maria-Dolores Pérez-Garcia, Sylvie Citerne, Renaud Sergheraert, Julie Lalande, Béatrice Teulat, Emmanuelle Mounier, Soulaiman Sakr and Jérémy Lothier
Int. J. Mol. Sci. 2022, 23(13), 7338; https://doi.org/10.3390/ijms23137338 - 30 Jun 2022
Cited by 6 | Viewed by 2062
Abstract
Water deficit causes substantial yield losses that climate change is going to make even more problematic. Sustainable agricultural practices are increasingly developed to improve plant tolerance to abiotic stresses. One innovative solution amongst others is the integration of plant biostimulants in agriculture. In [...] Read more.
Water deficit causes substantial yield losses that climate change is going to make even more problematic. Sustainable agricultural practices are increasingly developed to improve plant tolerance to abiotic stresses. One innovative solution amongst others is the integration of plant biostimulants in agriculture. In this work, we investigate for the first time the effects of the biostimulant –Leafamine®–a protein hydrolysate on greenhouse lettuce (Lactuca sativa L.) grown under well-watered and water-deficit conditions. We examined the physiological and metabolomic water deficit responses of lettuce treated with Leafamine® (0.585 g/pot) or not. Root application of Leafamine® increased the shoot fresh biomass of both well-watered (+40%) and deficit-irrigated (+20%) lettuce plants because the projected leaf area increased. Our results also indicate that Leafamine® application could adjust the nitrogen metabolism by enhancing the total nitrogen content, amino acid (proline) contents and the total protein level in lettuce leaves, irrespective of the water condition. Osmolytes such as soluble sugars and polyols, also increased in Leafamine®-treated lettuce. Our findings suggest that the protective effect of Leafamine is a widespread change in plant metabolism and could involve ABA, putrescine and raffinose. Full article
(This article belongs to the Special Issue State-of-the-Art Molecular Plant Sciences in France)
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13 pages, 3218 KiB  
Article
Compound-Specific 14N/15N Analysis of Amino Acid Trimethylsilylated Derivatives from Plant Seed Proteins
by Jean-Baptiste Domergue, Julie Lalande, Cyril Abadie and Guillaume Tcherkez
Int. J. Mol. Sci. 2022, 23(9), 4893; https://doi.org/10.3390/ijms23094893 - 28 Apr 2022
Cited by 2 | Viewed by 1977
Abstract
Isotopic analyses of plant samples are now of considerable importance for food certification and plant physiology. In fact, the natural nitrogen isotope composition (δ15N) is extremely useful to examine metabolic pathways of N nutrition involving isotope fractionations. However, δ15N [...] Read more.
Isotopic analyses of plant samples are now of considerable importance for food certification and plant physiology. In fact, the natural nitrogen isotope composition (δ15N) is extremely useful to examine metabolic pathways of N nutrition involving isotope fractionations. However, δ15N analysis of amino acids is not straightforward and involves specific derivatization procedures to yield volatile derivatives that can be analysed by gas chromatography coupled to isotope ratio mass spectrometry (GC-C-IRMS). Derivatizations other than trimethylsilylation are commonly used since they are believed to be more reliable and accurate. Their major drawback is that they are not associated with metabolite databases allowing identification of derivatives and by-products. Here, we revisit the potential of trimethylsilylated derivatives via concurrent analysis of δ15N and exact mass GC-MS of plant seed protein samples, allowing facile identification of derivatives using a database used for metabolomics. When multiple silylated derivatives of several amino acids are accounted for, there is a good agreement between theoretical and observed N mole fractions, and δ15N values are satisfactory, with little fractionation during derivatization. Overall, this technique may be suitable for compound-specific δ15N analysis, with pros and cons. Full article
(This article belongs to the Special Issue State-of-the-Art Molecular Plant Sciences in France)
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