Special Issue "Protein Biosensors"
Deadline for manuscript submissions: 30 September 2023 | Viewed by 657
2. URT-ISA, CNR at Department of Biology, University of Naples Federico II, 80126 Napoli, Italy
Interests: optical biosensors; fluorescence correlation spectroscopy; surface characterization
Protein-based biosensors represent one of the emerging methods for quickly analyzing the presence of target analytes in complex matrix samples. The use of proteins for the design of biosensors should ensure high selectivity and specificity. In fact, proteins are complex molecules that play many critical roles in cells. They are involved in most cellular functions, enabling the regular function of the cell. Protein-based biosensors can be constructed starting from a system with two nearly isoenergetic states, the equilibrium between which should be modulated by the binding of the target analyte. Desirable properties of proteins in such biosensors include: (a) conformational variations due to the binding of the target analyte should be large enough to be detected by the operator; (b) the system should be tunable so that target analytes with different concentrations can be detected over a large dynamic range; (c) conformational variations should be coupled to a sensitive output; and (d) the proteins should be stable in operative conditions under which biosensors should work.
Dr. Antonio Varriale
Dr. Maria Staiano
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