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Crop Molecular Breeding: Current Status and Future Directions

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Plant Sciences".

Deadline for manuscript submissions: 30 August 2024 | Viewed by 1232

Special Issue Editors


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Guest Editor
Key Laboratory of Oasis Eco-Agriculture, College of Agriculture, Shihezi University, Shihezi, China
Interests: cotton genetics; genomics; molecular breeding; genetic improvement
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

With the continuous advances in sequencing technology, the development of analytical tools for discovery, annotation and deciphering of genetic variants existing amongst diverse germplasm of every crop species, and precisely engineering target genes by genome editing, breeding new crop varieties is now entering a new era in which functional genetic variants can be rationally and rapidly combined and created as required to develop the next generation of crop varieties.

As an example, cotton (Gossypium spp.) is a multi-purpose crop, producing not only natural fiber for the global textile industry, but also oil and protein-rich seeds for downstream industries. Since the release of the first cotton reference genome (G. raimondii) in 2012, the genome sequences of 22 diploids and all 7 allotetraploids have been published, and thousands of accessions of both the diploid- and allotetraploid-cultivated cotton species have been re-sequenced. The genome data provide a rich source for investigating cotton evolution and finding genetic variants associated with important agronomic traits of cotton. As a result, not only marker-assisted selection but also genomic prediction has been adopted in cotton breeding, with the aim of increasing breeding efficiency and shortening the breeding cycle. Thus, we are launching a Special Issue on “Corp Molecular Breeding: Current Status and Future Directions” to present the accomplishments achieved in crop molecular genetics and genomics and to explore new opportunities in developing and deploying molecular tools in breeding the next generation of elite crop varieties.

This Special Issue will accept original research manuscripts reporting novel results on the topics mentioned above and covered by the keywords, as well as review and perspective articles on topics related to crop molecular breeding.

Dr. Qian-Hao Zhu
Prof. Dr. Jie Sun
Guest Editors

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

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Keywords

  • crop molecular breeding
  • marker-assisted breeding
  • genomics-enabled molecular breeding
  • QTL mapping and annotation
  • genome-wide association study
  • genomic prediction and selection
  • functional genomic variations
  • gene editing

Published Papers (1 paper)

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Research

25 pages, 6195 KiB  
Article
VdPT1 Encoding a Neutral Trehalase of Verticillium dahliae Is Required for Growth and Virulence of the Pathogen
by Lihua Chen, Xiaohu Ma, Tiange Sun, Qian-Hao Zhu, Hongjie Feng, Yongtai Li, Feng Liu, Xinyu Zhang, Jie Sun and Yanjun Li
Int. J. Mol. Sci. 2024, 25(1), 294; https://doi.org/10.3390/ijms25010294 - 25 Dec 2023
Viewed by 748
Abstract
Verticillum dahliae is a soil-borne phytopathogenic fungus causing destructive Verticillium wilt disease. We previously found a trehalase-encoding gene (VdPT1) in V. dahliae being significantly up-regulated after sensing root exudates from a susceptible cotton variety. In this study, we characterized the function [...] Read more.
Verticillum dahliae is a soil-borne phytopathogenic fungus causing destructive Verticillium wilt disease. We previously found a trehalase-encoding gene (VdPT1) in V. dahliae being significantly up-regulated after sensing root exudates from a susceptible cotton variety. In this study, we characterized the function of VdPT1 in the growth and virulence of V. dahliae using its deletion-mutant strains. The VdPT1 deletion mutants (ΔVdPT1) displayed slow colony expansion and mycelial growth, reduced conidial production and germination rate, and decreased mycelial penetration ability and virulence on cotton, but exhibited enhanced stress resistance, suggesting that VdPT1 is involved in the growth, pathogenesis, and stress resistance of V. dahliae. Host-induced silencing of VdPT1 in cotton reduced fungal biomass and enhanced cotton resistance against V. dahliae. Comparative transcriptome analysis between wild-type and mutant identified 1480 up-regulated and 1650 down-regulated genes in the ΔVdPT1 strain. Several down-regulated genes encode plant cell wall-degrading enzymes required for full virulence of V. dahliae to cotton, and down-regulated genes related to carbon metabolism, DNA replication, and amino acid biosynthesis seemed to be responsible for the decreased growth of the ΔVdPT1 strain. In contrast, up-regulation of several genes related to glycerophospholipid metabolism in the ΔVdPT1 strain enhanced the stress resistance of the mutated strain. Full article
(This article belongs to the Special Issue Crop Molecular Breeding: Current Status and Future Directions)
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