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Parasite Biology and Host-Parasite Interactions 2.0

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Biology".

Deadline for manuscript submissions: 30 May 2024 | Viewed by 1059

Special Issue Editors


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Guest Editor
Department of Animal, Plant and Soil Sciences, La Trobe University, Bundoora 3086, Australia
Interests: genetic variation in immunity to nematodes; selective breeding for disease resistance; host–parasite interactions; immunomodulation
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Department of Experimental Immunotherapy, Faculty of Medicine, Lazarski University, 02-662 Warsaw, Poland
Interests: nematodes; Heligmosomoides polygyrus bakeri infection model; immunomodulation; regulatory T cells; immunomodulatory proteins; autoimmune disorders
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

This Special Issue continues on from the previous edition, “Parasite Biology and Host-Parasite Interactions”.

Protozoan and metazoan parasites are a major cause of disease and death in humans and domestic animals, including livestock and wildlife. Host–parasite interactions are complex, with the host immune response recognizing hundreds of parasite molecules and parasites through multiple mechanisms and producing multiple molecules to manipulate the immune system. Nonetheless, there has been steady progress in understanding and controlling parasite infections.

This Special Issue will bring together original research articles and reviews to collate our knowledge of parasite biology, the interactions between hosts and parasites, and progress in parasite control and to highlight approaches that are proving to be productive. Although the focus of this issue is molecular interactions, clinical submissions presenting biomolecular experiments are welcome.

Prof. Dr. Michael Stear
Dr. Katarzyna Donskow-Lysoniewska
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • parasite
  • immunity
  • immunomodulation
  • helper T cells
  • IgA
  • IgE
  • eosinophils
  • mast cells
  • regulatory T cells
  • galectin

Published Papers (1 paper)

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Research

21 pages, 2105 KiB  
Article
The Development of Toxoplasma gondii Recombinant Trivalent Chimeric Proteins as an Alternative to Toxoplasma Lysate Antigen (TLA) in Enzyme-Linked Immunosorbent Assay (ELISA) for the Detection of Immunoglobulin G (IgG) in Small Ruminants
by Bartłomiej Tomasz Ferra, Maciej Chyb, Karolina Sołowińska, Lucyna Holec-Gąsior, Marta Skwarecka, Karolina Baranowicz and Justyna Gatkowska
Int. J. Mol. Sci. 2024, 25(8), 4384; https://doi.org/10.3390/ijms25084384 - 16 Apr 2024
Viewed by 767
Abstract
This study presents an evaluation of seventeen newly produced recombinant trivalent chimeric proteins (containing the same immunodominant fragment of SAG1 and SAG2 of Toxoplasma gondii antigens, and an additional immunodominant fragment of one of the parasite antigens, such as AMA1, GRA1, GRA2, GRA5, [...] Read more.
This study presents an evaluation of seventeen newly produced recombinant trivalent chimeric proteins (containing the same immunodominant fragment of SAG1 and SAG2 of Toxoplasma gondii antigens, and an additional immunodominant fragment of one of the parasite antigens, such as AMA1, GRA1, GRA2, GRA5, GRA6, GRA7, GRA9, LDH2, MAG1, MIC1, MIC3, P35, and ROP1) as a potential alternative to the whole-cell tachyzoite lysate (TLA) used in the detection of infection in small ruminants. These recombinant proteins, obtained by genetic engineering and molecular biology methods, were tested for their reactivity with specific anti-Toxoplasma IgG antibodies contained in serum samples of small ruminants (192 samples of sheep serum and 95 samples of goat serum) using an enzyme-linked immunosorbent assay (ELISA). The reactivity of six recombinant trivalent chimeric proteins (SAG1-SAG2-GRA5, SAG1-SAG2-GRA9, SAG1-SAG2-MIC1, SAG1-SAG2-MIC3, SAG1-SAG2-P35, and SAG1-SAG2-ROP1) with IgG antibodies generated during T. gondii invasion was comparable to the sensitivity of TLA-based IgG ELISA (100%). The obtained results show a strong correlation with the results obtained for TLA. This suggests that these protein preparations may be a potential alternative to TLA used in commercial tests and could be used to develop a cheaper test for the detection of parasite infection in small ruminants. Full article
(This article belongs to the Special Issue Parasite Biology and Host-Parasite Interactions 2.0)
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