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New Insights into Lactoferrin
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New Insights into Lactoferrin

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Immunology".

Deadline for manuscript submissions: 20 September 2024 | Viewed by 1424

Special Issue Editors

Dr. Antimo Cutone

E-Mail Website
Guest Editor
Department of Biosciences and Territory, University of Molise, 86090 Pesche, Italy
Interests: lactoferrin; iron homeostasis; iron-proteins; inflammation; oxidative stress; ferroportin; yeast; membrane proteins
Special Issues, Collections and Topics in MDPI journals
Dr. Luigi Rosa

E-Mail Website
Guest Editor
Department of Public Health and Infectious Diseases, University of Rome “La Sapienza”, 00185 Rome, Italy
Interests: lactoferrin; lactoferrin structure and functions, in vitro and in vivo bacterial infections; in vitro and in vivo viral infections; inflammation; iron and inflammatory homeostasis
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Lactoferrin (Lf), a positively charged glycoprotein known for its iron-binding ability produced by the exocrine glands and neutrophils. By virtue of its role in innate immunity, Lf exerts antibacterial, antiviral, antiparasitic, antifungal, antioxidant, anti-inflammatory, immunomodulatory and anticancer activities. The physicochemical characteristics of Lf can greatly impact its functionality, which can be influenced by species-specific and tissue-specific factors, as well as environmental conditions. This complexity highlights the different avenues of investigation in the Lf field. Importantly, Lf shows promise in the prevention and treatment of various pathological conditions, gaining recognition as a nutraceutical and as a carrier for the delivery of biologically active compounds.

This Special Issue is supervised by Dr. Antimo Cutone and Dr. Luigi Rosa, with assistance from our Topical Advisory Panel Member, Dr. Giusi Ianiro. It aims to provide a thorough review of biochemical, molecular and physiological aspects of Lf biology and its applications to both healthy and pathological people. This Special Issue will cover topics such as Lf’s structural and functional properties, role in disease prevention and treatment, as well as the development of new biotechnologies aimed at improving Lf production, bioavailability and functionality. Original research articles and reviews on these and related topics are welcome.

Dr. Antimo Cutone
Dr. Luigi Rosa
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • lactoferrin
  • lactoferrin structure
  • lactoferrin functions
  • infection
  • inflammation
  • iron homeostasis
  • oxidative stress
  • lactoferrin bioavailability

Published Papers (1 paper)

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Research

15 pages, 3990 KiB  
Article
Production of Bioactive Porcine Lactoferrin through a Novel Glucose-Inducible Expression System in Pichia pastoris: Unveiling Antimicrobial and Anticancer Functionalities
by Chih-Ching Yen, Pei-Ying Wu, Huan Ou-Yang, Hsiao-Ling Chen, Kowit-Yu Chong, Ro-Lin Chang and Chuan-Mu Chen
Int. J. Mol. Sci. 2024, 25(3), 1818; https://doi.org/10.3390/ijms25031818 - 02 Feb 2024
Viewed by 1110
Abstract
Lactoferrin (LF) stands as one of the extensively investigated iron-binding glycoproteins within milk, exhibiting diverse biological functionalities. The global demand for LF has experienced consistent growth. Biotechnological strategies aimed at enhancing LF productivity through microbial expression systems offer substantial cost-effective advantages and exhibit [...] Read more.
Lactoferrin (LF) stands as one of the extensively investigated iron-binding glycoproteins within milk, exhibiting diverse biological functionalities. The global demand for LF has experienced consistent growth. Biotechnological strategies aimed at enhancing LF productivity through microbial expression systems offer substantial cost-effective advantages and exhibit fewer constraints compared to traditional animal bioreactor technologies. This study devised a novel recombinant plasmid, wherein the AOX1 promoter was replaced with a glucose-inducible G1 promoter (PG1) to govern the expression of recombinant porcine LF (rpLF) in Pichia pastoris GS115. High-copy-number PG1-rpLF yeast clones were meticulously selected, and subsequent induction with 0.05 g/L glucose demonstrated robust secretion of rpLF. Scaling up production transpired in a 5 L fermenter, yielding an estimated rpLF productivity of approximately 2.8 g/L by the conclusion of glycerol-fed fermentation. A three-step purification process involving tangential-flow ultrafiltration yielded approximately 6.55 g of rpLF crude (approximately 85% purity). Notably, exceptional purity of rpLF was achieved through sequential heparin and size-exclusion column purification. Comparatively, the present glucose-inducible system outperformed our previous methanol-induced system, which yielded a level of 87 mg/L of extracellular rpLF secretion. Furthermore, yeast-produced rpLF demonstrated affinity for ferric ions (Fe3+) and exhibited growth inhibition against various pathogenic microbes (E. coli, S. aureus, and C. albicans) and human cancer cells (A549, MDA-MB-231, and Hep3B), similar to commercial bovine LF (bLF). Intriguingly, the hydrolysate of rpLF (rpLFH) manifested heightened antimicrobial and anticancer effects compared to its intact form. In conclusion, this study presents an efficient glucose-inducible yeast expression system for large-scale production and purification of active rpLF protein with the potential for veterinary or medical applications. Full article
(This article belongs to the Special Issue New Insights into Lactoferrin)
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