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Dear Colleagues,

This Special Issue is a continuation of our previous successful Special Issue “Organoids: The New 3D-Frontier to Model Different Diseases In Vitro”.

Organoids are miniaturized replicates of human organs that recreate their architecture and physiology. They are generated from patient biopsy samples or from induced pluripotent stem cells and provide an unprecedented tool to model organogenesis and human diseases. Organoids have been used to study genetic disorders and infectious diseases, as well as for drug screening. They represent a valid alternative or complement to animal models, because it is complex to extrapolate results from model systems to humans. On the other hand, it is well known that biological processes specific to the human body cannot be modelled in other animals. Moreover, organoids might become a useful tool in the perspective of personalized medicine and therapy.

This Special Issue calls for papers presenting the implementation of organoid technology and offering novel insights into organogenesis, as well as into the pathophysiology of diseases.

Dr. Alessandra Cazzaniga
Dr. Sara Castiglioni
Guest Editors

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Keywords

organoids
organoid technology
brain
gut
liver
organogenesis
vascularization
metabolism
inflammation
stress
cancer

Published Papers (1 paper)

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15 pages, 2226 KiB

Open AccessArticle

Traces of Canine Inflammatory Bowel Disease Reflected by Intestinal Organoids

by Barbara Pratscher, Benno Kuropka, Georg Csukovich, Pavlos G. Doulidis, Katrin Spirk, Nina Kramer, Patricia Freund, Alexandro Rodríguez-Rojas and Iwan A. Burgener

Int. J. Mol. Sci. 2024, 25(1), 576; https://doi.org/10.3390/ijms25010576 - 01 Jan 2024

Viewed by 1048

Abstract

Inflammatory bowel disease (IBD) is a chronic inflammatory condition that affects humans and several domestic animal species, including cats and dogs. In this study, we have analyzed duodenal organoids derived from canine IBD patients using quantitative proteomics. Our objective was to investigate whether these organoids show phenotypic traits of the disease compared with control organoids obtained from healthy donors. To this aim, IBD and control organoids were subjected to quantitative proteomics analysis via liquid chromatography–mass spectrometry. The obtained data revealed notable differences between the two groups. The IBD organoids exhibited several alterations at the levels of multiple proteins that are consistent with some known IBD alterations. The observed phenotype in the IBD organoids to some degree mirrors the corresponding intestinal condition, rendering them a compelling approach for investigating the disease and advancing drug exploration. Additionally, our study revealed similarities to some human IBD biomarkers, further emphasizing the translational and comparative value of dogs for future investigations related to the causes and treatment of IBD. Relevant proteins such as CALU, FLNA, MSN and HMGA2, which are related to intestinal diseases, were all upregulated in the IBD duodenal organoids. At the same time, other proteins such as intestinal keratins and the mucosal immunity PIGR were depleted in these IBD organoids. Based on these findings, we propose that these organoids could serve as a valuable tool for evaluating the efficacy of therapeutic interventions against canine IBD. Full article

(This article belongs to the Special Issue Organoids: The New 3D-Frontier to Model Different Diseases In Vitro 2.0)

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