International Journal of Molecular Sciences

Journal Browser

► Journal Browser

Molecular Characterization of Plant-Virus Interactions

Share This Special Issue

Special Issue Editor

Special Issue Information

Dear Colleagues,

I am editing a Special Issue in the International Journal of Molecular Science related to the molecular characterization of plant–virus interactions.

Plant viruses as obligate parasites require the host cell machinery for their multiplication, while the host cell will try to defend itself, avoiding virus multiplication. The elucidation of all these different mechanisms required for virus multiplication are of obvious fundamental interest but can additionally provide information on the host factors involved, as potential antiviral targets. Plant viruses cause significant economic losses in agriculture at regional and global levels, being responsible for a significant proportion of devastating emergent and re-emergent plant diseases, and molecular plant virology can provide solutions. Additionally, plant virology studies can contribute to the general advancement of plant sciences through the molecular characterization of basic plant biological processes.

This Special Issue is open to all researchers studying plant virus multiplication in host cells at the molecular level. Papers are welcome as original research articles, as well as review papers including recent advancements.

Dr. Manuel Miras is a scientist (Topical Advisory Panel in IJMS), who will assist Dr. Truniger in managing this Special Issue.

Dr. Veronica Truniger
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

plant virus
host cell
molecular mechanism
replication
translation
defence
infection

Published Papers (1 paper)

Order results

Result details

Show export options Show export options

Select all

Export citation of selected articles as:

Research

17 pages, 2511 KiB

Open AccessArticle

Advances in Understanding the Mechanism of Cap-Independent Cucurbit Aphid-Borne Yellows Virus Protein Synthesis

by Verónica Truniger, Giuliano Sting Pechar and Miguel A. Aranda

Int. J. Mol. Sci. 2023, 24(24), 17598; https://doi.org/10.3390/ijms242417598 - 18 Dec 2023

Viewed by 1125

Abstract

Non-canonical translation mechanisms have been described for many viral RNAs. In the case of several plant viruses, their protein synthesis is controlled by RNA elements in their genomic 3′-ends that are able to enhance cap-independent translation (3′-CITE). The proposed general mechanism of 3′-CITEs includes their binding to eukaryotic translation initiation factors (eIFs) that reach the 5′-end and AUG start codon through 5′-3′-UTR-interactions. It was previously shown that cucurbit aphid-borne yellows virus (CABYV) has a 3′-CITE, which varies in sequence and structure depending on the phylogenetic group to which the isolate belongs, possibly as a result of adaptation to the different geographical regions. In this work, the cap-independent translation mechanisms of two CABYV 3′-CITEs belonging to the Mediterranean (CMTE) and Asian (CXTE) groups, respectively, were studied. In vivo cap-independent translation assays show that these 3′-CITEs require the presence of the CABYV short genomic 5′-UTR with at least 40% adenines in cis and an accessible 5′-end for its activity. Additionally, they suggest that the eIF4E-independent CABYV 3′-CITE activities may not require either eIF4A or the eIF4F complex, but may depend on eIF4G and PABP. By pulling down host proteins using RNA baits containing both 5′- and 3′-CABYV-UTRs, 80 RNA binding proteins were identified. These interacted preferentially with either CMTE, CXTE, or both. One of these proteins, specifically interacting with the RNA containing CMTE, was HSP70.2. Preliminary results suggested that HSP70.2 may be involved in CMTE- but not CXTE-mediated cap-independent translation activity. Full article

(This article belongs to the Special Issue Molecular Characterization of Plant-Virus Interactions)

► Show Figures

Journal Menu

Journal Browser

Molecular Characterization of Plant-Virus Interactions

Share This Special Issue

Special Issue Editor

Special Issue Information

Keywords

Published Papers (1 paper)

Research

Further Information

Guidelines

MDPI Initiatives

Follow MDPI