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Recent Advances on Fluorescent Probes and Imaging in Cell Molecular Biology

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Biology".

Deadline for manuscript submissions: 30 June 2024 | Viewed by 862

Special Issue Editor


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Guest Editor
Unidad de Microscopía Confocal, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain
Interests: imaging; cell biology; bioinformatics; computational biology; fluorescence; cancer research; microscopy; HCS

Special Issue Information

Dear Colleagues,

Recent advances in fluorescent probes and their use in imaging have given rise to a new era of applications with an impact on scientific research to monitor biological processes in vivo, as well as in medical uses, both for diagnostics and treatment.

New strategies for sample fluorescent codification play a central role in spatial transcriptomics and genomics, combining the molecular information with its spatial location and thus favoring a better understanding of the mechanisms underlying the different pathologies in complex and heterogeneous cellular environments. Additionally, using alternative illumination strategies and/or sample processing allows us to obtain static information from deeper tissue layers and observe dynamic changes in living samples.

The mixing of new imaging techniques with modern fluorescent probes and artificial intelligence analysis is indeed expanding the possibilities, rendering more and better quality results in basic and translational studies.

This Special Issue is dedicated to all important advances in the field of imaging with new fluorescent staining strategies and their use for specific biological/therapeutic applications.

Original research articles, reviews, and perspectives from experts in academic and industrial fields describing and discussing the most recent advancements are welcome.

Dr. Diego Megias
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • imaging
  • fluorescence
  • cell biology
  • microscopy
  • cell molecular imaging

Published Papers (1 paper)

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Research

14 pages, 4544 KiB  
Article
A Molecular Hybrid of the GFP Chromophore and 2,2′-Bipyridine: An Accessible Sensor for Zn2+ Detection with Fluorescence Microscopy
by Attila Csomos, Miklós Madarász, Gábor Turczel, Levente Cseri, Gergely Katona, Balázs Rózsa, Ervin Kovács and Zoltán Mucsi
Int. J. Mol. Sci. 2024, 25(6), 3504; https://doi.org/10.3390/ijms25063504 - 20 Mar 2024
Viewed by 483
Abstract
The few commercially available chemosensors and published probes for in vitro Zn2+ detection in two-photon microscopy are compromised by their flawed spectroscopic properties, causing issues in selectivity or challenging multistep syntheses. Herein, we present the development of an effective small molecular GFP [...] Read more.
The few commercially available chemosensors and published probes for in vitro Zn2+ detection in two-photon microscopy are compromised by their flawed spectroscopic properties, causing issues in selectivity or challenging multistep syntheses. Herein, we present the development of an effective small molecular GFP chromophore-based fluorescent chemosensor with a 2,2′-bipyridine chelator moiety (GFZnP BIPY) for Zn2+ detection that has straightforward synthesis and uncompromised properties. Detailed experimental characterizations of the free and the zinc-bound compounds within the physiologically relevant pH range are presented. Excellent photophysical characteristics are reported, including a 53-fold fluorescence enhancement with excitation and emission maxima at 422 nm and 492 nm, respectively. A high two-photon cross section of 3.0 GM at 840 nm as well as excellent metal ion selectivity are reported. In vitro experiments on HEK 293 cell culture were carried out using two-photon microscopy to demonstrate the applicability of the novel sensor for zinc bioimaging. Full article
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