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Cells
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Stem Cells in Organoid Technology
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Stem Cells in Organoid Technology

A special issue of Cells (ISSN 2073-4409). This special issue belongs to the section "Stem Cells".

Deadline for manuscript submissions: closed (15 April 2023) | Viewed by 6038

Special Issue Editor

Dr. Min Young Lee

E-Mail Website
Guest Editor
Department of Molecular Physiology, College of Pharmacy, Kyungpook National University, Daegu 41566, Republic of Korea
Interests: stem cells; cardiomyocytes

Special Issue Information

Dear Colleagues,

Stem cells can be divided into two main forms: pluripotent stem cells, including embryonic stem cells, and induced pluripotent stem cells, which can differentiate into any cell in the body. Adult stem cells are multipotent, which means they can only change into some cells in the body, not any cell. Their unique characteristics to differentiate into other cell types (pluripotency) allow us to use stem cells as valuable tools for research and therapeutic applications. During the past few decades, stem cell differentiation technology has made remarkable progress. Additionally, organoid technology has recently been developed. Organoids are three-dimensional cell structures grown in vitro from stem cells, primarily isolated from biopsies or pluripotent stem cells, which recapitulate key features of native organs from a histological and functional perspective. Organoids can be widely utilized for various purposes, such as organ development, disease modeling, drug development, regenerative medicine, etc.

This Special Issue of Cells will focus on the development of innovative technologies in the generation of pluripotent stem-cell-derived organoids and their application in disease modeling, as well as regenerative medicine. 

Dr. Min Young Lee
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Cells is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • pluripotent stem cells
  • cerebral organoid
  • midbrain organoid
  • Alzheimer’s disease
  • Parkinson’s disease
  • cardiac differentiation
  • neural differentiation
  • tissue repair

Published Papers (2 papers)

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Research

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19 pages, 4623 KiB  
Article
Sorting and Manipulation of Human PGC-LC Using PDPN and Hanging Drop Cultures
by Brahim Arkoun, Pauline Moison, Marie-Justine Guerquin, Sébastien Messiaen, Delphine Moison, Sophie Tourpin, Christelle Monville and Gabriel Livera
Cells 2022, 11(23), 3832; https://doi.org/10.3390/cells11233832 - 29 Nov 2022
Viewed by 1746
Abstract
The generation of oocytes from induced pluripotent stem cells (iPSCs) was proven efficient with mouse cells. However, no human iPSCs have yet been reported to generate cells able to complete oogenesis. Additionally, efficient sorting of human Primordial Germ Cell-like Cells (hPGC-LCs) without [...] Read more.
The generation of oocytes from induced pluripotent stem cells (iPSCs) was proven efficient with mouse cells. However, no human iPSCs have yet been reported to generate cells able to complete oogenesis. Additionally, efficient sorting of human Primordial Germ Cell-like Cells (hPGC-LCs) without genomic integration of fluorescent reporter for their downstream manipulation is still lacking. Here, we aimed to develop a model that allows human germ cell differentiation in vitro in order to study the developing human germline. The hPGC-LCs specified from two iPS cell lines were sorted and manipulated using the PDPN surface marker without genetic modification. hPGC-LCs obtained remain arrested at early stages of maturation and no further differentiation nor meiotic onset occurred when these were cultured with human or mouse fetal ovarian somatic cells. However, when cultured independently of somatic ovarian cells, using BMP4 and the hanging drop-transferred EBs system, early hPGC-LCs further differentiate efficiently and express late PGC (DDX4) and meiotic gene markers, although no SYCP3 protein was detected. Altogether, we characterized a tool to sort hPGC-LCs and an efficient in vitro differentiation system to obtain pre-meiotic germ cell-like cells without using a gonadal niche. Full article
(This article belongs to the Special Issue Stem Cells in Organoid Technology)
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Review

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19 pages, 1241 KiB  
Review
Pluripotent Stem Cells as a Model for Human Embryogenesis
by Daniela Ávila-González, Mikel Ángel Gidi-Grenat, Guadalupe García-López, Alejandro Martínez-Juárez, Anayansi Molina-Hernández, Wendy Portillo, Néstor Emmanuel Díaz-Martínez and Néstor Fabián Díaz
Cells 2023, 12(8), 1192; https://doi.org/10.3390/cells12081192 - 20 Apr 2023
Viewed by 3912
Abstract
Pluripotent stem cells (PSCs; embryonic stem cells and induced pluripotent stem cells) can recapitulate critical aspects of the early stages of embryonic development; therefore, they became a powerful tool for the in vitro study of molecular mechanisms that underlie blastocyst formation, implantation, the [...] Read more.
Pluripotent stem cells (PSCs; embryonic stem cells and induced pluripotent stem cells) can recapitulate critical aspects of the early stages of embryonic development; therefore, they became a powerful tool for the in vitro study of molecular mechanisms that underlie blastocyst formation, implantation, the spectrum of pluripotency and the beginning of gastrulation, among other processes. Traditionally, PSCs were studied in 2D cultures or monolayers, without considering the spatial organization of a developing embryo. However, recent research demonstrated that PSCs can form 3D structures that simulate the blastocyst and gastrula stages and other events, such as amniotic cavity formation or somitogenesis. This breakthrough provides an unparalleled opportunity to study human embryogenesis by examining the interactions, cytoarchitecture and spatial organization among multiple cell lineages, which have long remained a mystery due to the limitations of studying in utero human embryos. In this review, we will provide an overview of how experimental embryology currently utilizes models such as blastoids, gastruloids and other 3D aggregates derived from PSCs to advance our understanding of the intricate processes involved in human embryo development. Full article
(This article belongs to the Special Issue Stem Cells in Organoid Technology)
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