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Biosensors
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Nanomaterial-Based Biosensors for Food Analysis
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Nanomaterial-Based Biosensors for Food Analysis

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor Materials".

Deadline for manuscript submissions: closed (30 November 2022) | Viewed by 20517

Special Issue Editors

Prof. Dr. Jinkai Zheng

E-Mail Website
Guest Editor
Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Interests: plant foods; processing; functional components; analysis techniques; structure; nutrition and health; gut microbiota; in vivo metabolism; molecular mechanisms
Special Issues, Collections and Topics in MDPI journals
Dr. Longhua Tang

E-Mail Website
Guest Editor
College of Optical Science and Engineering, State Key Laboratory of Modern Optical Instrumentation, Zhejiang University, Hangzhou 310027, China
Interests: optical sensor; photonic biosensor; tunnelling sensor; electrochemical biosensor; biochip
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Rapid analytical methods are essential for monitoring food quality and safety. Up to now, the research and development of simpler and faster analytical procedures based on biosensors has aroused much interest due to their simplicity and sensitivity. Nanomaterials, such as carbon nanomaterials (carbon nanotubes and graphene), metal nanoparticles, nanowires, nanocomposites, and nanostructured metal oxide nanoparticles have recently had a great impact on the development of biosensors. The nanomaterials are used as catalytic tools, immobilization platforms, or as optical or electroactive labels to improve the biosensing performance and obtain higher sensitivity, stability, and selectivity. To capture the latest developments in this direction, the specific topics of the Special Issue include, but are not limited to, the following:

  1. Nanomaterial-based biosensors for food composition analysis;
  2. Nanomaterial-based biosensors for food safety analysis;
  3. Nanomaterial-based biosensors for health biomarker analysis.

This Special Issue welcomes research findings, rapid communications, and full reviews.

You may choose our joint special issue in Sensors.

Prof. Dr. Jinkai Zheng
Prof. Dr. Longhua Tang
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • food analysis
  • quality
  • safety
  • health biomarker
  • nanomaterials
  • biosensors
  • rapid detection

Related Special Issue

Published Papers (7 papers)

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Research

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13 pages, 2066 KiB  
Article
Screening of Single-Stranded DNA Aptamer Specific for Florfenicol and Application in Detection of Food Safety
by Minghui Shi, Ruobing Liu, Fuyuan Zhang, Bimal Chitrakar and Xianghong Wang
Biosensors 2022, 12(9), 701; https://doi.org/10.3390/bios12090701 - 01 Sep 2022
Cited by 4 | Viewed by 1939
Abstract
In this work, the single-stranded DNA (ssDNA) aptamers specific to florfenicol (FF) and having a high binding affinity were prepared using the magnetic bead-based systematic evolution of ligands by the exponential enrichment technique (MB-SELEX). After 10 rounds of the MB-SELEX screening, aptamers that [...] Read more.
In this work, the single-stranded DNA (ssDNA) aptamers specific to florfenicol (FF) and having a high binding affinity were prepared using the magnetic bead-based systematic evolution of ligands by the exponential enrichment technique (MB-SELEX). After 10 rounds of the MB-SELEX screening, aptamers that can simultaneously recognize FF and its metabolite florfenicol amine (FFA) were obtained. The aptamer with the lowest dissociation constant (Kd) was truncated and optimized based on a secondary structure analysis. The optimal aptamer selected was Apt-14t, with a length of 43 nt, and its dissociation constant was 4.66 ± 0.75 nM, which was about 7 times higher than that of the full-length sequence. The potential binding sites and interactions with FF were demonstrated by molecular docking simulations. In addition, a colorimetric strategy for nanogold aptamers was constructed. The linear detection range of this method was 0.00128–500 ng/mL and the actual detection limit was 0.00128 ng/mL. Using this strategy to detect florfenicol in actual milk and eggs samples, the spiked recoveries were 88.9–123.1% and 84.0–112.2%, respectively, and the relative standard deviation was less than 5.6%, showing high accuracy. Full article
(This article belongs to the Special Issue Nanomaterial-Based Biosensors for Food Analysis)
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11 pages, 1827 KiB  
Article
Based on Unmodified Aptamer-Gold Nanoparticles Colorimetric Detection of Dexamethasone in Food
by Yadi Qin, Hayilati Bubiajiaer, Jun Yao and Minwei Zhang
Biosensors 2022, 12(4), 242; https://doi.org/10.3390/bios12040242 - 14 Apr 2022
Cited by 10 | Viewed by 2906
Abstract
Residue and illegal addition of Dexamethasone (DEX) in food has received widespread attention over the past few decades. Long-term intake of DEX will have a strong endocrine-disrupting effect, and there is an urgent need to develop highly sensitive and rapid on-site detection methods. [...] Read more.
Residue and illegal addition of Dexamethasone (DEX) in food has received widespread attention over the past few decades. Long-term intake of DEX will have a strong endocrine-disrupting effect, and there is an urgent need to develop highly sensitive and rapid on-site detection methods. In this work, a colorimetric sensor based on an unmodified aptamer and gold nanoparticles (Au NPs) was designed to detect DEX in milk and glucosamine. Under optimized conditions, the absorbance ratio of Au NPs increased linearly with DEX concentration over the range of 10–350 nmol/mL (r2 = 0.997), with a limit of detection (LOD) of 0.5 nmol/mL, and the recoveries ranged from 93.6 to 117%. To explore the interaction mechanism between aptamer and DEX, molecular docking and molecular dynamics simulations were applied to probe intermolecular interactions and structures of the complex. The establishment of aptamer-based sensors effectively avoids the antibody screening response, with a cost-efficient, excellent selective and great potential in DEX determination. Full article
(This article belongs to the Special Issue Nanomaterial-Based Biosensors for Food Analysis)
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15 pages, 5123 KiB  
Article
Synthesis and Physicochemical Characterization of Polymer Film-Based Anthocyanin and Starch
by Kana Husna Erna, Wen Xia Ling Felicia, Joseph Merillyn Vonnie, Kobun Rovina, Koh Wee Yin and Md Nasir Nur’Aqilah
Biosensors 2022, 12(4), 211; https://doi.org/10.3390/bios12040211 - 01 Apr 2022
Cited by 19 | Viewed by 3673
Abstract
Colorimetric indicators, used in food intelligent packaging, have enormous promise for monitoring and detecting food quality by analyzing and interpreting the quality data of packaged food. Hence, our study developed and characterized a biopolymer film based on starch and anthocyanin for prospective meat [...] Read more.
Colorimetric indicators, used in food intelligent packaging, have enormous promise for monitoring and detecting food quality by analyzing and interpreting the quality data of packaged food. Hence, our study developed and characterized a biopolymer film based on starch and anthocyanin for prospective meat freshness monitoring applications. The developed film was morphologically characterized using different morphology instruments to identify the interaction between anthocyanin and starch. The color differences of the proposed film in response to different pH buffers have also been investigated. The combination of anthocyanin and starch produces a smooth and homogenous surface with an intermolecular hydrogen bond that increases the biopolymer’s wavelength. The film indicated bright red at pH 2.0–6.0, bluish-grey at pH 7.0–11.0, and yellowish-green above 11.0 that the naked eye can see. The indicator film shows high sensitivity toward pH changes. The inclusion of anthocyanin increases the biopolymer film’s thickness and crystalline condition with low humidity, water solubility, and swelling values. As a result, the polymer film can be employed in the food industry as an affordable and environmentally friendly indication of meat freshness. Full article
(This article belongs to the Special Issue Nanomaterial-Based Biosensors for Food Analysis)
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13 pages, 4272 KiB  
Article
Bio-Specific Au/Fe3+ Porous Spongy Nanoclusters for Sensitive SERS Detection of Escherichia coli O157:H7
by Yuzhi Li, Fei Gao, Chang Lu, Marie-Laure Fauconnier and Jinkai Zheng
Biosensors 2021, 11(10), 354; https://doi.org/10.3390/bios11100354 - 24 Sep 2021
Cited by 14 | Viewed by 2290
Abstract
For sensitive and fast detection of Escherichia coli O157:H7, organic and inorganic hybrid Au/Fe3+ nanoclusters (NCs) were synthesized for the first time using gold nanoparticles (GNPs), bovine serum albumin, ferric chloride, phosphate-buffered saline, and antibodies. The Au/Fe3+ porous spongy NCs with [...] Read more.
For sensitive and fast detection of Escherichia coli O157:H7, organic and inorganic hybrid Au/Fe3+ nanoclusters (NCs) were synthesized for the first time using gold nanoparticles (GNPs), bovine serum albumin, ferric chloride, phosphate-buffered saline, and antibodies. The Au/Fe3+ porous spongy NCs with large surface area showed excellent bio-specific capability for E. coli O157:H7. GNPs in Au/Fe3+ NCs functioned as signal enhancers, significantly increasing the Raman signal via the metathesis reaction product of Prussian blue and obviously improving the detection sensitivity. We combined the novel Au/Fe3+ NCs with antibody-modified magnetic nanoparticles to create a biosensor capable of sensitive detection of E. coli O157:H7, which showed a good linear response (101 to 106 cfu/mL), high detection sensitivity (2 cfu/mL), and good recovery rate (93.60–97.50%) in spiked food samples. These results make the biosensor well-suited for food safety monitoring. This strategy achieves the goal of sensitive and quantitative detection of E. coli O157:H7. Full article
(This article belongs to the Special Issue Nanomaterial-Based Biosensors for Food Analysis)
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10 pages, 2152 KiB  
Article
Ratiometric G-Quadruplex Assay for Robust Lead Detection in Food Samples
by Yumei Liu, Hao Yang, Rui Wan, Mohammad Rizwan Khan, Nan Wang, Rosa Busquets, Ruijie Deng, Qiang He and Zhifeng Zhao
Biosensors 2021, 11(8), 274; https://doi.org/10.3390/bios11080274 - 16 Aug 2021
Cited by 15 | Viewed by 3043
Abstract
Lead (Pb2+) pollution is a serious food safety issue, rapid detection of Pb2+ residual in food is vital to guarantee food quality and safety. Here we proposed ratiometric aptamer probes, allowing robust Pb2+ supervision in food samples. Pb2+ [...] Read more.
Lead (Pb2+) pollution is a serious food safety issue, rapid detection of Pb2+ residual in food is vital to guarantee food quality and safety. Here we proposed ratiometric aptamer probes, allowing robust Pb2+ supervision in food samples. Pb2+ specific aptamer can bolster a transition of G-quadruplex structural response to Pb2+; this process can be monitored by N-methyl mesoporphyrin IX (NMM), which is highly specific to G-quadruplex. Particularly, the utilization of G-quadruplex specific dye and terminal-labeled fluorophore allowed to endue ratiometric signal outputs towards Pb2+, dramatically increase the robustness for lead detection. The ratiometric G-quadruplex assay allowed a facile and one-pot Pb2+ detection at room temperature using a single-stranded DNA aptamer. We demonstrated its feasibility for detecting lead pollution in fresh eggs and tap water samples. The ratiometric G-quadruplex design is expected to be used for on-site Pb2+ testing associated with food safety. Full article
(This article belongs to the Special Issue Nanomaterial-Based Biosensors for Food Analysis)
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12 pages, 2273 KiB  
Article
Point-of-Care Detection of Salivary Nitrite Based on the Surface Plasmon-Assisted Catalytic Coupling Reaction of Aromatic Amines
by Chen Zhao, Ruyi Shi, Jiale Wu, Xuan Luo and Xiangjiang Liu
Biosensors 2021, 11(7), 223; https://doi.org/10.3390/bios11070223 - 05 Jul 2021
Cited by 4 | Viewed by 3012
Abstract
Rapid quantification of nitrite (NO2−) in food, drink and body fluids is of significant importance for both food safety and point-of-care (POA) applications. However, conventional nitrite analytical methods are complicated, constrained to sample content, and time-consuming. Inspired by a nitrite-triggered [...] Read more.
Rapid quantification of nitrite (NO2−) in food, drink and body fluids is of significant importance for both food safety and point-of-care (POA) applications. However, conventional nitrite analytical methods are complicated, constrained to sample content, and time-consuming. Inspired by a nitrite-triggered surface plasmon-assisted catalysis (SPAC) reaction, a rapid point-of-care detection salivary nitrate was developed in this work. NO2− ions can trigger the rapid conversion of p-aminothiophenol (PATP) to p,p′-dimercaptozaobenzene (DMAB) on gold nanoparticles (GNPs) under light illumination, and the emerged new bands at ca. 1140, 1390, 1432 cm−1 originating from DMAB can be used to the quantification of nitrite. Meanwhile, to make the method entirely suitable for on-site fast screen or point-of-care application, the technique is needed to be further optimized. The calibration graph for nitrates was linear in the range of 1–100 µM with a correlation coefficient of 0.9579. The limit of detection was 1 µM. The facile method could lead to a further understanding of the progression and treatment of periodontitis and to guide professionals in planning on-site campaigns to effectively control periodontal diseases. Full article
(This article belongs to the Special Issue Nanomaterial-Based Biosensors for Food Analysis)
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Review

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16 pages, 4383 KiB  
Review
Research Progress in the Synthesis of Carbon Dots and Their Application in Food Analysis
by Yuan Yu, Lili Zhang, Xin Gao, Yuanmiao Feng, Hongyuan Wang, Caihong Lei, Yanhong Yan and Shuiping Liu
Biosensors 2022, 12(12), 1158; https://doi.org/10.3390/bios12121158 - 12 Dec 2022
Cited by 3 | Viewed by 2179
Abstract
Food safety is connected to public health, making it crucial to protecting people’s health. Food analysis and detection can assure food quality and effectively reduce the entry of harmful foods into the market. Carbon dots (CDs) are an excellent choice for food analysis [...] Read more.
Food safety is connected to public health, making it crucial to protecting people’s health. Food analysis and detection can assure food quality and effectively reduce the entry of harmful foods into the market. Carbon dots (CDs) are an excellent choice for food analysis and detection attributable to their advantages of good optical properties, water solubility, high chemical stability, easy functionalization, excellent bleaching resistance, low toxicity, and good biocompatibility. This paper focuses on the optical properties, synthesis methods, and applications of CDs in food analysis and detection, including the recent advances in food nutritional composition analysis and food quality detection, such as food additives, heavy metal ions, foodborne pathogens, harmful organic pollutants, and pH value. Moreover, this review also discusses the potentially toxic effects, current challenges, and prospects of CDs in basic research and applications. We hope that this review can provide valuable information to lay a foundation for subsequent research on CDs and promote the exploration of CDs-based sensing for future food detection. Full article
(This article belongs to the Special Issue Nanomaterial-Based Biosensors for Food Analysis)
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