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Biosensors
Special Issues
CRISPR/Cas-Based Biosensing Systems: Development and Applications
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Special Issue "CRISPR/Cas-Based Biosensing Systems: Development and Applications"

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensors and Healthcare".

Deadline for manuscript submissions: 31 January 2024 | Viewed by 823

Special Issue Editor

Dr. Ki Soo Park

E-Mail Website
Guest Editor
Department of Biological Engineering, Konkuk University, Seoul 05029, Republic of Korea
Interests: nucleic acid nanotechnology; molecular diagnostics; exosome analysis platform; biosensing; theranostics
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) protein, which originated as a bacterial and archaeal defense mechanism, has become an essential tool in various fields, including genome editing, gene expression modulation, cell imaging, and biosensing. Especially, it has shown great promise in biosensing applications due to its easy design, simple operation, and high specificity. Until now, there have been many attempts to develop biosensing platforms for the sensitive detection of target nucleic acids in combination with various signal amplification strategies. Furthermore, the high modularity of CRISPR/Cas protein enables the expansion of detection targets even to small molecules, proteins, exosomes, cells, etc., advancing the area of biosensing.

In this Special Issue, we aim to explore recent advances in CRISPR/Cas-based biosensing systems that can improve the quality of human life. We encourage the submission of papers in the following topics described by the keywords below but also welcome works on related topics.

Dr. Ki Soo Park
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • nucleic acid nanotechnology
  • isothermal amplification
  • molecular diagnostics
  • immunological diagnostics
  • point-of-care testing
  • biosensors
  • in vitro diagnostics
  • clinical applications
  • CRISPR/Cas protein

Published Papers (1 paper)

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Research

11 pages, 2134 KiB  
Article
CRISPR/Cas12a Collateral Cleavage Activity for Sensitive 3′–5′ Exonuclease Assay
by
Jae Hoon Jeung
,
Hyogu Han
,
Chang Yeol Lee
and
Jun Ki Ahn
Biosensors 2023, 13(11), 963; https://doi.org/10.3390/bios13110963 - 30 Oct 2023
Viewed by 640
Abstract
This study presents a technique for detecting 3′–5′ exonuclease activity through the use of CRISPR/Cas12a. These enzymes, including 3′–5′ exonuclease (Exo III), perform crucial roles in various cellular processes and are associated with life expectancy. However, imbalances in their expression can increase susceptibility [...] Read more.
This study presents a technique for detecting 3′–5′ exonuclease activity through the use of CRISPR/Cas12a. These enzymes, including 3′–5′ exonuclease (Exo III), perform crucial roles in various cellular processes and are associated with life expectancy. However, imbalances in their expression can increase susceptibility to diseases such as cancer, particularly under prolonged stress. In this study, an activator sequence of CRISPR/Cas12a was constructed on the 5′–end of a hairpin probe (HP), forming a blunt end. When the 3′–end of the HP was hydrolyzed with Exo III activity, the activator sequence of Cas12a was exposed, which led to collateral cleavage of the DNA signal probe and generated a fluorescent signal, allowing sensitive and highly specific Exo III detection. This detection principle relied on the fact that Exo III exclusively cleaves the 3′–end mononucleotide of dsDNA and does not affect ssDNA. Based on this strategy, Exo III activity was successfully assayed at 0.0073 U/mL, demonstrating high sensitivity. In addition, this technique was used to screen candidate inhibitors of Exo III activity. Full article
(This article belongs to the Special Issue CRISPR/Cas-Based Biosensing Systems: Development and Applications)
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