Graphene-Based Biosensing

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor Materials".

Deadline for manuscript submissions: closed (15 September 2021) | Viewed by 7804

Special Issue Editors

Shandong Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou 253023, China
Interests: graphene; biosensor; field-effect transistor; SERS; SPR; fluorescence biosensor; electrochemiluminescence
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Melbourne Centre for Nanofabrication, Victorian Node of the Australian National Fabrication Facility, Clayton, VIC 3168, Australia
Interests: three-dimensional metamaterials; photonic crystals; integrated system designs; integrated optics; nano-imaging technology; nanophotonic devices
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Federal Research Center, Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences, Institute of Biophysics, SB RAS, Krasnoyarsk, Russia
Interests: bioluminescent proteins (structure, properties, modification, application); bioluminescence-based analytical systems; label-free analytical systems
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Laboratory of Bioluminescent and Environmental Technologies, Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, 660036 Krasnoyarsk, Russia
Interests: bioluminescence; coelenterazine-dependent luciferases; Ca2+-regulated photoproteins; DNA/RNA aptamers; binding assay; label-free analytical systems

Special Issue Information

Dear Colleagues,

Benefiting from the distinct transduction property, graphene has been viewed as one of the most promising materials for the fabrication of rapid and sensitive sensors for biological quantity detection.

The high-quality and low-cost of graphene have been developed very quickly in the past few years, which has paved the way for the development of many different biosensors. However, research into grephene-based sensors are still in their infancy and far from real applications due to the difficulties in fabricating devices with good uniformity and reproducibility. Therefore, more effort needs to be paid on the following aspects of this field. Firstly, it is critical to have a better understanding of interactions between graphene and varieties of interfaces, molecules, and cells. Secondly, the miniaturization and functionalization of graphene-based sensors should be investigated to facilitate the fabrication of biosensors in arrays that can be used for highly sensitive, selective, and high-throughput sensing. Thirdly, specific detections of the biological sensors are of critical importance to their practical applications. The reported sensors are normally tested in strictly controlled conditions while many interference factors widely existing in the real environment have not been considered. So, the selectivity and stability of the graphene-based sensors need to be characterized and optimized in a real-time environment analyzing and in vitro or in vivo biological sensing. Moreover, some unexpected results may come from the impurities or defects of graphene. So, the techniques for synthesizing high-quality graphene need to be developed. Significant efforts should be devoted to developing facile strategies for controllable, reproducible, and scalable synthesis and the functionalization of graphene with defined properties.

The purpose of this Special Issue is to present the state of recent progress in this field, including the new fabrication methods of graphene, graphene-based sensors, and the various applications of graphene-based sensors. The Special Issue covers various aspects of theoretical and experimental researches related to graphene-based sensors. We welcome all the submissions relating to recent progress in graphene-based sensors.

Potential topics include, but are not limited to, the following:

Graphene in field-effect transistors for biosensors;

Graphene used for surface enhanced Raman scattering (SERS);

Graphene-based electrochemical, impedance and electrochemiluminescence biosensors;

Fluorescence biosensors based on graphene;

Graphene-based fiber optic surface plasmon resonance (SPR) for biosensors.

Dr. Shicai Xu
Dr. Guangyuan Si
Prof. Dr. Liudmila A Frank
Dr. Vasilisa V. Krasitskaya
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • graphene
  • biosensor
  • field-effect transistor
  • SERS
  • SPR
  • fluorescence biosensor
  • electrochemiluminescence

Published Papers (2 papers)

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12 pages, 4700 KiB  
Article
Highly Sensitive Uric Acid Detection Based on a Graphene Chemoresistor and Magnetic Beads
by Wangyang Zhang, Xiaoqiang Zhao, Lina Diao, Hao Li, Zhonghao Tong, Zhiqi Gu, Bin Miao, Zhan Xu, Han Zhang, Yue Wu and Jiadong Li
Biosensors 2021, 11(9), 304; https://doi.org/10.3390/bios11090304 - 29 Aug 2021
Cited by 9 | Viewed by 3749
Abstract
In this study, we developed a low-cost, reusable, and highly sensitive analytical platform for the detection of the human metabolite uric acid (UA). This novel analysis platform combines the graphene chemoresistor detection technique with a magnetic bead (MB) system. The heterojunction (single-layer graphene [...] Read more.
In this study, we developed a low-cost, reusable, and highly sensitive analytical platform for the detection of the human metabolite uric acid (UA). This novel analysis platform combines the graphene chemoresistor detection technique with a magnetic bead (MB) system. The heterojunction (single-layer graphene and HfO2 thin-film material) of our graphene-based biosensor worked as a transducer to detect the pH change caused by the specific catalytic reaction between UA and uricase, and hence acquires a UA concentration. Immobilization of uricase on MBs can decouple the functionalization steps from the sensor surface, which allows the sensor to be reusable. Our microsensor platform exhibits a relatively lower detection limit (1 μM), high sensitivity (5.6 mV/decade), a linear range (from 1 μM to 1000 μM), and excellent linearity (R2 = 0.9945). In addition, interference assay and repeatability tests were conducted, and the result suggests that our method is highly stable and not affected by common interfering substances (glucose and urea). The integration of this high-performance and compact biosensor device can create a point-of-care diagnosis system with reduced cost, test time, and reagent volume. Full article
(This article belongs to the Special Issue Graphene-Based Biosensing)
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Review

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15 pages, 5087 KiB  
Review
Super-Resolution Imaging with Graphene
by Xiaoxiao Jiang, Lu Kong, Yu Ying, Qiongchan Gu, Jiangtao Lv, Zhigao Dai and Guangyuan Si
Biosensors 2021, 11(9), 307; https://doi.org/10.3390/bios11090307 - 30 Aug 2021
Cited by 5 | Viewed by 3005
Abstract
Super-resolution optical imaging is a consistent research hotspot for promoting studies in nanotechnology and biotechnology due to its capability of overcoming the diffraction limit, which is an intrinsic obstacle in pursuing higher resolution for conventional microscopy techniques. In the past few decades, a [...] Read more.
Super-resolution optical imaging is a consistent research hotspot for promoting studies in nanotechnology and biotechnology due to its capability of overcoming the diffraction limit, which is an intrinsic obstacle in pursuing higher resolution for conventional microscopy techniques. In the past few decades, a great number of techniques in this research domain have been theoretically proposed and experimentally demonstrated. Graphene, a special two-dimensional material, has become the most meritorious candidate and attracted incredible attention in high-resolution imaging domain due to its distinctive properties. In this article, the working principle of graphene-assisted imaging devices is summarized, and recent advances of super-resolution optical imaging based on graphene are reviewed for both near-field and far-field applications. Full article
(This article belongs to the Special Issue Graphene-Based Biosensing)
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