Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
4.9
5.4
Journals
Biosensors
Special Issues
Plasmonic Biosensors for Biomedical Applications
share announcement

Plasmonic Biosensors for Biomedical Applications

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensors and Healthcare".

Deadline for manuscript submissions: 30 June 2024 | Viewed by 3006

Special Issue Editors

Dr. Sónia O. Pereira

E-Mail Website
Guest Editor
i3N, Department of Physics, University of Aveiro, Aveiro, Portugal
Interests: nanotechnology; nanoscience; biotechnology; polymers; biofunctionalization
Dr. Cátia Leitão

E-Mail Website
Guest Editor
i3N, Department of Physics, University of Aveiro, Aveiro, Portugal
Interests: optical fiber sensors; biosensors; optical biosensors; physiological monitoring; fiber bragg gratings; optical fibers technology
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The research in the field of biosensors has experienced a huge increase in recent years, mainly due to the high demand for rapid and simple-to-handle solutions for medical diagnosis in the point of care. Biosensing techniques based on the plasmonic phenomenon have been known to be very promising approaches as they are highly sensitive and could reach low limits of detection.

The collaboration of scientists from different fields (biochemistry, nanoscience, materials, and electronics, among others) has been playing an important role in the development of such devices, from the synthesis of the materials, and the biofunctionalization procedures, to the data processing and analysis.

This Special Issue aims to highlight the latest advances concerning the different steps in the production of plasmonic biosensors for biomedical applications. Therefore, articles including (but not limited to) the following topics are welcome: the production of novel plasmonic nanostructures/materials with promising SPR and LSPR properties for biosensing; studies on the exploration of biofunctionalization procedures for the preparation of specific biorecognition layers; production of plasmonic biosensing devices and plasmonic data analysis; and data science to improve their sensing performance (e.g., machine learning) and integration as small and portable devices.

Dr. Sónia O. Pereira
Dr. Cátia Leitão
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biosensing
  • LSPR
  • SPR
  • medical biomarkers
  • medical diagnosis
  • point-of-care testing
  • healthcare

Published Papers (3 papers)

Download All Papers
Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

13 pages, 3871 KiB  
Article
Detection of NT-proBNP Using Optical Fiber Back-Reflection Plasmonic Biosensors
by Ana Sofia Assunção, Miguel Vidal, Maria João Martins, Ana Violeta Girão, Médéric Loyez, Christophe Caucheteur, José Mesquita-Bastos, Florinda M. Costa, Sónia O. Pereira and Cátia Leitão
Biosensors 2024, 14(4), 173; https://doi.org/10.3390/bios14040173 - 04 Apr 2024
Viewed by 590
Abstract
Heart failure (HF) is a clinical entity included in cardiovascular diseases affecting millions of people worldwide, being a leading cause of hospitalization of older adults, and therefore imposing a substantial economic burden on healthcare systems. HF is characterized by dyspnea, fatigue, and edema [...] Read more.
Heart failure (HF) is a clinical entity included in cardiovascular diseases affecting millions of people worldwide, being a leading cause of hospitalization of older adults, and therefore imposing a substantial economic burden on healthcare systems. HF is characterized by dyspnea, fatigue, and edema associated with elevated blood levels of natriuretic peptides, such as N Terminal pro-B-type Natriuretic Peptide (NT-proBNP), for which there is a high demand for point of care testing (POCT) devices. Optical fiber (OF) biosensors offer a promising solution, capable of real-time detection, quantification, and monitoring of NT-proBNP concentrations in serum, saliva, or urine. In this study, immunosensors based on plasmonic uncladded OF tips were developed using OF with different core diameters (200 and 600 µm). The tips were characterized to bulk refractive index (RI), anddetection tests were conducted with NT-proBNP concentrations varying from 0.01 to 100 ng/mL. The 200 µm sensors showed an average total variation of 3.6 ± 2.5 mRIU, an average sensitivity of 50.5 mRIU/ng·mL−1, and a limit of detection (LOD) of 0.15 ng/mL, while the 600 µm sensors had a response of 6.1 ± 4.2 mRIU, a sensitivity of 102.8 mRIU/ng·mL−1, and an LOD of 0.11 ng/mL. Control tests were performed using interferents such as uric acid, glucose, and creatinine. The results show the potential of these sensors for their use in biological fluids. Full article
(This article belongs to the Special Issue Plasmonic Biosensors for Biomedical Applications)
►▼ Show Figures

Figure 1

14 pages, 1819 KiB  
Article
Critical Issues on the Surface Functionalization of Plasmonic Au-Ag/TiO2 Thin Films with Thiolated Oligonucleotide-Based Biorecognition Elements
by Diogo Costa, Patrícia Pereira-Silva, Paulo Sousa, Vânia Pinto, Joel Borges, Filipe Vaz, Graça Minas and Paula Sampaio
Biosensors 2024, 14(4), 159; https://doi.org/10.3390/bios14040159 - 27 Mar 2024
Viewed by 569
Abstract
This work reports on the surface functionalization of a nanomaterial supporting localized surface plasmon resonances (LSPRs) with (synthetic) thiolated oligonucleotide-based biorecognition elements, envisaging the development of selective LSPR-based DNA biosensors. The LSPR thin-film transducers are composed of noble metal nanoparticles (NPs) embedded in [...] Read more.
This work reports on the surface functionalization of a nanomaterial supporting localized surface plasmon resonances (LSPRs) with (synthetic) thiolated oligonucleotide-based biorecognition elements, envisaging the development of selective LSPR-based DNA biosensors. The LSPR thin-film transducers are composed of noble metal nanoparticles (NPs) embedded in a TiO2 dielectric matrix, produced cost-effectively and sustainably by magnetron sputtering. The study focused on the immobilization kinetics of thiolated oligonucleotide probes as biorecognition elements, followed by the evaluation of hybridization events with the target probe. The interaction between the thiolated oligonucleotide probe and the transducer’s surface was assessed by monitoring the LSPR signal with successive additions of probe solution through a microfluidic device. The device was specifically designed and fabricated for this work and adapted to a high-resolution LSPR spectroscopy system with portable characteristics. Benefiting from the synergetic characteristics of Ag and Au in the form of bimetallic nanoparticles, the Au-Ag/TiO2 thin film proved to be more sensitive to thiolated oligonucleotide binding events. Despite the successful surface functionalization with the biorecognition element, the detection of complementary oligonucleotides revealed electrostatic repulsion and steric hindrance, which hindered hybridization with the target oligonucleotide. This study points to an effect that is still poorly described in the literature and affects the design of LSPR biosensors based on nanoplasmonic thin films. Full article
(This article belongs to the Special Issue Plasmonic Biosensors for Biomedical Applications)
►▼ Show Figures

Figure 1

12 pages, 2229 KiB  
Article
LSPR-Based Aptasensor for Rapid Urinary Detection of NT-proBNP
by Maria António, Rui Vitorino and Ana L. Daniel-da-Silva
Biosensors 2023, 13(7), 736; https://doi.org/10.3390/bios13070736 - 17 Jul 2023
Cited by 2 | Viewed by 1276
Abstract
N-terminal pro-brain natriuretic peptide (NT-proBNP) is a myocardial stress biomarker that can be found in serum or plasma, saliva, and urine in the context of cardiovascular disease. In this study, we developed a rapid (~25 min) and straightforward localized surface plasmon resonance (LSPR)-based [...] Read more.
N-terminal pro-brain natriuretic peptide (NT-proBNP) is a myocardial stress biomarker that can be found in serum or plasma, saliva, and urine in the context of cardiovascular disease. In this study, we developed a rapid (~25 min) and straightforward localized surface plasmon resonance (LSPR)-based assay for detecting NT-proBNP in urine. The assay employs citrate-capped gold nanoparticles (AuNPs) and an aptamer specific for NT-proBNP, which initially interacts with NT-proBNP. The remaining unbound aptamer then interacts with the AuNPs, and the addition of NaCl induces the aggregation of the unprotected AuNPs, resulting in a decrease in absorbance at the LSPR band (A521) and an increase in absorbance at 750 nm (A750). The concentration of NT-proBNP showed a linear correlation with the aggregation ratio (A521/A750), and the assay demonstrated a limit of detection (LOD) of 0.303 µg·L−1 and a detection range of 0.566–8 µg·L−1. However, the presence of sulfur-containing proteins in saliva and fetal bovine serum hindered the detection of NT-proBNP in these biofluids. Nevertheless, the assay successfully detected NT-proBNP in diluted urine with an LOD of 0.417 µg·L−1 and a detection range of 0.589–6 µg·L−1. The observed values in urine samples from preterm infants with cardiovascular disease fell within this range, indicating the potential clinical relevance of the assay. The recovery percentages ranged from 92.3 to 116.3%. Overall, our findings suggest that the LSPR-based assay for NT-proBNP detection in urine can be a valuable tool for the diagnosis and treatment of cardiovascular disease. Full article
(This article belongs to the Special Issue Plasmonic Biosensors for Biomedical Applications)
►▼ Show Figures

Figure 1

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-3
Back to TopTop