Pathogenic Mechanism and Biosafety of Pathogenic Microorganisms

A special issue of Biomedicines (ISSN 2227-9059). This special issue belongs to the section "Molecular and Translational Medicine".

Deadline for manuscript submissions: 30 September 2024 | Viewed by 1188

Special Issue Editor


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Guest Editor
School of Basic Medical Sciences, Fudan University, Shanghai, China
Interests: pathogenic microbial pathogenesis; biosafety

Special Issue Information

Dear Colleagues,

The title of this Special Issue, “Pathogenic Mechanisms and Biosafety of Pathogenic Microorganisms”, refers to understanding how these organisms can cause diseases in humans, animals, and plants, as well as the safety measures that should be implemented to prevent their spread. This involves studying the molecular and cellular mechanisms that enable these microorganisms to infect hosts and cause disease. Additionally, researchers also need to focus on developing effective control measures, including the development of vaccines, antibiotics, and other treatments. Biosafety measures are also crucial to prevent the accidental or intentional release of these microorganisms, which could have devastating consequences for public health and the environment. Therefore, it is vital to continually investigate and understand the pathogenic mechanisms and biosafety of pathogenic microorganisms to mitigate the potential harm they may cause.

Dr. Xia Cai
Guest Editor

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Published Papers (1 paper)

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Research

11 pages, 4845 KiB  
Article
Aedes aegypti Beta-1,3-Glucan-Binding Protein Inhibits Dengue and ZIKA Virus Replication
by Xiaoxue Xie, Di Wang, Bo Li, Guorui Liang, Xiaoli Chen, Dan Xing, Teng Zhao, Xinyu Zhou and Chunxiao Li
Biomedicines 2024, 12(1), 88; https://doi.org/10.3390/biomedicines12010088 - 01 Jan 2024
Cited by 1 | Viewed by 995
Abstract
GNBPB6, a beta-1,3-glucan-binding protein, was identified in the transcriptome of Aedes aegypti (A. aegypti) with dengue (DENV), Zika (ZIKV), and chikungunya viruses (CHIKV). In this study, we not only clarified that DENV2 and ZIKV regulate the changes in GNBPB6 expression but [...] Read more.
GNBPB6, a beta-1,3-glucan-binding protein, was identified in the transcriptome of Aedes aegypti (A. aegypti) with dengue (DENV), Zika (ZIKV), and chikungunya viruses (CHIKV). In this study, we not only clarified that DENV2 and ZIKV regulate the changes in GNBPB6 expression but also identified the relationship of this gene with viral infections. The changes in GNBPB6 expression were quantified and showed a decrease in A. aegypti cells (Aag2 cells) at 2 dpi and 3 dpi and an increase at 4 dpi and 5 dpi (p < 0.05). A significant increase was observed only at 5 dpi after DENV2 infection. Subsequently, a GNBPB6 knockout (KO) cell line was constructed using the CRISPR/Cas9 system, and the DENV2 and ZIKV RNA copies, along with cell densities, were quantified and compared between the KO and wild type (WT) cells at different dpi. The result showed that DENV2 and ZIKV RNA copies were significantly increased in the KO cell line with no significant change in cell growth. Finally, DENV2 copies decreased after GNBPB6 was complemented in the KO. In conclusion, GNBPB6 knockout and complementation in Aag2 cells revealed that GNBPB6 can inhibit the replication of both DENV2 and ZIKV. These results contribute to subsequent research on mosquito–virus interactions. Full article
(This article belongs to the Special Issue Pathogenic Mechanism and Biosafety of Pathogenic Microorganisms)
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