Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
4.0
4.2
Journals
Biology
Special Issues
Advances in Biological Research of Adipose-Derived Stem Cells
share announcement

Advances in Biological Research of Adipose-Derived Stem Cells

A special issue of Biology (ISSN 2079-7737). This special issue belongs to the section "Cell Biology".

Deadline for manuscript submissions: 31 December 2024 | Viewed by 4297

Special Issue Editor

Dr. Xinlin Yang

E-Mail Website
Guest Editor
Department of Orthopaedic Surgery, University of Virginia, Charlottesville, VA 22908, USA
Interests: tissue engineering; stem cell biology; inflammation; bone diseases; osteoarthritis and bone defect; formyl peptide receptors

Special Issue Information

Dear Colleagues,

Adipose-derived stem cells (ADSCs), a subset of adult stem cells obtained from the processing of adipose tissue, have many desirable qualities. These cells are able to differentiate into various cell types, including adipocytes, osteocytes, chondrocytes, and myocytes. The primary objective of this Special Issue is to establish a platform for the distribution of advanced research and innovation in the biology and therapeutic use of ADSCs in a variety of conditions including bone disorders, diabetes mellitus, soft tissue reconstruction, and heart disease. This objective is in accordance with the broader aims of our journal: to publish innovative research within the field of medical biology in which multiple disciplines are involved.

In this Special Issue, original research articles and reviews are welcome. Research areas may include (but are not limited to) the following: isolation and culture method; biomarkers and multipotency; immunomodulation; and applications in regenerative medicine and tissue engineering.

Dr. Xinlin Yang
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biology is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • adipose-derived stem cells (ADSCs)
  • isolation and culture method
  • biomarkers and multipotency
  • immunomodulation
  • regenerative medicine
  • tissue engineering

Published Papers (3 papers)

Download All Papers
Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

Jump to: Review

13 pages, 2283 KiB  
Article
Preconditioning with Substance P Restores Therapeutic Efficacy of Aged ADSC by Elevating TNFR2 and Paracrine Potential
by Jiyuan Piao, Hyunchan Cho, Jong Hyun Park, Ki Hyun Yoo, Ildoo Jeong and Hyun Sook Hong
Biology 2023, 12(12), 1458; https://doi.org/10.3390/biology12121458 - 22 Nov 2023
Viewed by 921
Abstract
Aging leads to a decline in stem cell activity by reducing the repopulation rate and paracrine potential, ultimately diminishing efficacy in vivo. TNF-α can exert inflammatory and cell death actions via Erk by binding to TNFR-1, and survival and tissue repair actions via [...] Read more.
Aging leads to a decline in stem cell activity by reducing the repopulation rate and paracrine potential, ultimately diminishing efficacy in vivo. TNF-α can exert inflammatory and cell death actions via Erk by binding to TNFR-1, and survival and tissue repair actions via Akt by binding to TNFR-2. Aged cells are reported to have insufficient expression of TNFR-2, indicating that aged adipose-derived stem cells (ADSCs-E) lack the ability for cell survival and immune control compared to young ADSCs (ADSCs-Y). This study aims to assess the preconditioning effect of SP on the response of ADSCs-E to inflammation. ADSCs-E were treated with SP and then exposed to a high dose of TNF-α for 24 h. Consequently, ADSC-E exhibited weaker viability and lower TNFR2 levels compared to ADSC-Y. In response to TNF-α, the difference in TNFR2 expression became more pronounced in ADSC-E and ADSC-Y. Moreover, ADSC-E showed a severe deficiency in proliferation and paracrine activity. However, preconditioning with SP significantly enhanced the viability of ADSCs-E and also restored TNFR2 expression and paracrine potential, similar to ADSC-Y under inflammatory conditions. Our findings support the idea that preconditioning with SP has the potential to restore the cellular function of senescent stem cells before transplantation. Full article
(This article belongs to the Special Issue Advances in Biological Research of Adipose-Derived Stem Cells)
►▼ Show Figures

Graphical abstract

23 pages, 4992 KiB  
Article
Characterization of Rabbit Mesenchymal Stem/Stromal Cells after Cryopreservation
by Sai Koung Ngeun, Miki Shimizu and Masahiro Kaneda
Biology 2023, 12(10), 1312; https://doi.org/10.3390/biology12101312 - 07 Oct 2023
Cited by 1 | Viewed by 1374
Abstract
Adipose tissues (ADPs) are an alternative source for mesenchymal stem/stromal cells (MSCs), given that conventional bone marrow (BM) collection is painful and yields limited cell numbers. As the need for easily accessible MSCs grows, cryopreservation’s role in regenerative medicine is becoming increasingly vital. [...] Read more.
Adipose tissues (ADPs) are an alternative source for mesenchymal stem/stromal cells (MSCs), given that conventional bone marrow (BM) collection is painful and yields limited cell numbers. As the need for easily accessible MSCs grows, cryopreservation’s role in regenerative medicine is becoming increasingly vital. However, limited research exists on the characteristics and functional properties of rabbit-derived MSCs from various anatomical sources before and after cryopreservation. We examined the effects of cryopreservation using Bambanker. We found that cryopreservation did not adversely affect the morphology, viability, and adipogenic or chondrogenic differentiation abilities of ADP MSCs or BM MSCs. However, there was a notable drop in the proliferation rate and osteogenic differentiation capability of BM MSCs post-cryopreservation. Additionally, after cryopreservation, the surface marker gene expression of CD90 was not evident in ADP MSCs. As for markers, ADIPOQ can serve as an adipogenic marker for ADP MSCs. ACAN and CNMD can act as chondrogenic markers, but these two markers are not as effective post-cryopreservation on ADP MSCs, and osteogenic markers could not be validated. The study highlights that compared to BM MSCs, ADP MSCs retained a higher viability, proliferation rate, and differentiation potential after cryopreservation. As such, in clinical MSC use, we must consider changes in post-cryopreservation cell functions. Full article
(This article belongs to the Special Issue Advances in Biological Research of Adipose-Derived Stem Cells)
►▼ Show Figures

Figure 1

Review

Jump to: Research

28 pages, 4215 KiB  
Review
White Adipose Tissue Heterogeneity in the Single-Cell Era: From Mice and Humans to Cattle
by Hunter Ford, Qianglin Liu, Xing Fu and Clarissa Strieder-Barboza
Biology 2023, 12(10), 1289; https://doi.org/10.3390/biology12101289 - 27 Sep 2023
Cited by 1 | Viewed by 1719
Abstract
Adipose tissue is a major modulator of metabolic function by regulating energy storage and by acting as an endocrine organ through the secretion of adipokines. With the advantage of next-generation sequencing-based single-cell technologies, adipose tissue has been studied at single-cell resolution, thus providing [...] Read more.
Adipose tissue is a major modulator of metabolic function by regulating energy storage and by acting as an endocrine organ through the secretion of adipokines. With the advantage of next-generation sequencing-based single-cell technologies, adipose tissue has been studied at single-cell resolution, thus providing unbiased insight into its molecular composition. Recent single-cell RNA sequencing studies in human and mouse models have dissected the transcriptional cellular heterogeneity of subcutaneous (SAT), visceral (VAT), and intramuscular (IMAT) white adipose tissue depots and revealed unique populations of adipose tissue progenitor cells, mature adipocytes, immune cell, vascular cells, and mesothelial cells that play direct roles on adipose tissue function and the development of metabolic disorders. In livestock species, especially in bovine, significant gaps of knowledge remain in elucidating the roles of adipose tissue cell types and depots on driving the pathogenesis of metabolic disorders and the distinct fat deposition in VAT, SAT, and IMAT in meat animals. This review summarizes the current knowledge on the transcriptional and functional cellular diversity of white adipose tissue revealed by single-cell approaches and highlights the depot-specific function of adipose tissue in different mammalian species, with a particular focus on recent findings and future implications in cattle. Full article
(This article belongs to the Special Issue Advances in Biological Research of Adipose-Derived Stem Cells)
►▼ Show Figures

Figure 1

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-3
Back to TopTop