Preconditioning with Substance P Restores Therapeutic Efficacy of Aged ADSC by Elevating TNFR2 and Paracrine Potential
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Cell Culture
2.3. Preparation of Cell Extracts and Western Blot Analysis
2.4. SP and TNF-α Treatment
2.5. WST-1 Assay
2.6. Cytokine Measurement
2.7. Tube Formation Assay
2.8. Statistical Analysis
3. Results
3.1. ADSC-E Is More Susceptible to Inflammatory Stress Than ADSC-Y
3.2. TNF-α Treatment Reduces TNFR2 Expression in ADSC-E, Impairing Repopulation and Paracrine Potentials
3.3. Pretreatment with SP Blocks the Loss of Cell Viability in ADSC-E When Exposed to TNF-α, and This Is Accompanied by an Increase in TNFR2 and Angiocrine Factors
3.4. Preconditioning with SP Sustains ADSC-E in a State of Active Akt Signaling, Rather Than Erk Signaling, in Response to TNF-α Treatment
3.5. Preconditioning with SP Enhance Paracrine Potential of ADSC-E under Inflammatory Condition
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Piao, J.; Cho, H.; Park, J.H.; Yoo, K.H.; Jeong, I.; Hong, H.S. Preconditioning with Substance P Restores Therapeutic Efficacy of Aged ADSC by Elevating TNFR2 and Paracrine Potential. Biology 2023, 12, 1458. https://doi.org/10.3390/biology12121458
Piao J, Cho H, Park JH, Yoo KH, Jeong I, Hong HS. Preconditioning with Substance P Restores Therapeutic Efficacy of Aged ADSC by Elevating TNFR2 and Paracrine Potential. Biology. 2023; 12(12):1458. https://doi.org/10.3390/biology12121458
Chicago/Turabian StylePiao, Jiyuan, Hyunchan Cho, Jong Hyun Park, Ki Hyun Yoo, Ildoo Jeong, and Hyun Sook Hong. 2023. "Preconditioning with Substance P Restores Therapeutic Efficacy of Aged ADSC by Elevating TNFR2 and Paracrine Potential" Biology 12, no. 12: 1458. https://doi.org/10.3390/biology12121458