New Advances in Molecular Diagnostics of Crop Pathogens

A special issue of Biology (ISSN 2079-7737). This special issue belongs to the section "Plant Science".

Deadline for manuscript submissions: closed (30 April 2024) | Viewed by 2453

Special Issue Editors


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MED–Mediterranean Institute for Agriculture, Environment and Development & CHANGE–Global Change and Sustainability Institute, Institute for Advanced Studies and Research, Universidade de Évora, Évora, Portugal
Interests: plant protection; disease resistance; molecular diagnosis of plant pathogens
Special Issues, Collections and Topics in MDPI journals

E-Mail Website
Guest Editor
MED—Mediterranean Institute for Agriculture, Environment and Development & CHANGE—Global Change and Sustainability Institute, Institute for Advanced Studies and Research, Universidade de Évora, Pólo da Mitra, Ap. 94, 7006-554 Évora, Portugal
Interests: plant tolerance to stress; plant symbiosis; plant epigenetics
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
MED–Mediterranean Institute for Agriculture, Environment and Development & CHANGE–Global Change and Sustainability Institute, Departamento de Fitotecnia, Escola de Ciências e Tecnologia, Universidade de Évora, Évora, Portugal
Interests: plant pathology; pathogens and antagonists; molecular diagnostic tests; plant protection
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Agricultural crops are threatened by a wide variety of diseases that might compromise entire harvests. In recent decades, great advances in the knowledge of causal pathogens (e.g., bacteria, oomycetes, fungi, nematodes), their epidemiology, impact on crops, and ways of control have been achieved. In this sense, advanced disease detection and prevention are imperative to minimize crop losses, and specific treatments can be tailored to combat specific pathogens if they are identified early and/or plant diseases are diagnosed correctly. These need-based treatments allow maximizing productivity and ensuring agricultural sustainability. Furthermore, pathogen detection is of significant interest for the screening of resistant crop plants in view of plant breeding.

Advances in molecular biology, plant pathology, and biotechnology have allowed the development of methodologies that make diagnosis possible in terms of pathogenic agents’ identification and disease detection. These studies include well-established molecular methods as well as emerging methodologies, boosted by the advent of new technologies and the application of several OMIC platforms.

For this Special Issue, we invite the submission of original research papers and reviews focusing on cutting-edge knowledge around the use of molecular mechanisms for crop plant disease diagnosis, including the precise and rapid detection and identification of infecting pathogens to facilitate effective management of diseases.

Dr. Maria Doroteia Campos
Dr. Catarina Campos
Dr. Maria do Rosário Félix
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biology is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

 

Keywords

  • pathogen detection
  • pathogen identification
  • crop protection
  • crop diseases

Published Papers (1 paper)

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Research

12 pages, 1810 KiB  
Article
A TaqMan® Assay Allows an Accurate Detection and Quantification of Fusarium spp., the Causal Agents of Tomato Wilt and Rot Diseases
by Maria Doroteia Campos, Carla Varanda, Mariana Patanita, Joana Amaro Ribeiro, Catarina Campos, Patrick Materatski, André Albuquerque and Maria do Rosário Félix
Biology 2023, 12(2), 268; https://doi.org/10.3390/biology12020268 - 08 Feb 2023
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Abstract
In tomato plants, Fusarium spp. have been increasingly associated with several wilt and rot diseases that are responsible for severe yield losses. Here, we present a real-time PCR TaqMan® MGB (Minor Groove Binder) assay to detect and discriminate Fusarium spp. from other [...] Read more.
In tomato plants, Fusarium spp. have been increasingly associated with several wilt and rot diseases that are responsible for severe yield losses. Here, we present a real-time PCR TaqMan® MGB (Minor Groove Binder) assay to detect and discriminate Fusarium spp. from other fungal species that affect tomato plants. The methodology used is based on the selective amplification of the internal transcribed spacer (ITS) region of Fusarium spp. This assay revealed to be highly specific and sensitive for Fusarium species, targeting only the 29 Fusarium isolates from the 45 tested isolates associated to tomato diseases. Sensitivity was assessed with serial dilutions of Fusarium genomic DNA, with the limit of detection of 3.05 pg. An absolute DNA quantification method was also established, based on the determination of the absolute number of target copies. Finally, the effectiveness of the assay was successfully validated with the detection and quantification of Fusarium spp. in potentially infected tomato plants from an experimental field and in control plants grown under controlled conditions. The established methodology allows a reliable, sensitive, and reproducible estimation of Fusarium accumulation in infected tomato plants, gaining new insights for disease control and providing an additional tool in the screening of resistant plants. Full article
(This article belongs to the Special Issue New Advances in Molecular Diagnostics of Crop Pathogens)
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