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Bioengineering
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Stem Cell-Based Technology for Personalized Medicine Solutions
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Stem Cell-Based Technology for Personalized Medicine Solutions

A special issue of Bioengineering (ISSN 2306-5354). This special issue belongs to the section "Regenerative Engineering".

Deadline for manuscript submissions: closed (31 January 2024) | Viewed by 5740

Special Issue Editors

Dr. Giuseppe Pettinato

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Guest Editor
Harvard Medical School/Beth Israel Deaconess Medical Center, Boston, MA, USA
Interests: stem cell differentiation; organoids generation; tissue bioengineering
Dr. Giuseppe Ietto

E-Mail Website
Guest Editor
Emergency and Transplant Surgery Department, University of Insubria, 21100 Varese, Italy
Interests: kidney transplantation; surgery transplantation; dialysis; oncology; immunology
Special Issues, Collections and Topics in MDPI journals
Dr. Xuejun Wen

E-Mail Website
Guest Editor
Department of Chemical and Life Science Engineering, School of Engineering, Virginia Commonwealth University, Richmond, VA 23284, USA
Interests: bomaterials; cell tissue engineering; biomedical devices; nanomaterials

Special Issue Information

Dear Colleagues,

Personalized stem cell-based therapy represents the potential future for the treatment of degenerative diseases that cause the loss of organ function. Through the application of bioengineering technologies, it is possible to recreate in vitro cell products that resemble the real organ, called organoids. This promising technology has already introduced several bioengineered tissues to the clinical trial level, and more can be done to cover the demand for different organs that can be subject to functional failure. However, this very advanced field still faces challenges in making these organoids fully functional and in protecting them from the host immune system to prevent rejection after transplantation. In this Special Issue of Bioengineering on Stem Cell-Based Technology for Personalized Medicine Solutions, we will present what the latest research has to offer in regenerative medicine, stem cell technology, and in the bioengineering of tissues and materials, with experts from all over the world presenting their contributions to this very important scientific field.

Dr. Giuseppe Pettinato
Dr. Giuseppe Ietto
Dr. Xuejun Wen
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Bioengineering is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • stem cells
  • organoids
  • differentiation

Published Papers (3 papers)

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Research

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13 pages, 6676 KiB  
Article
Adipose Stromal Cell Spheroids for Cartilage Repair: A Promising Tool for Unveiling the Critical Maturation Point
by Azzurra Sargenti, Simone Pasqua, Marco Leu, Laura Dionisi, Giuseppe Filardo, Brunella Grigolo, Daniele Gazzola, Spartaco Santi and Carola Cavallo
Bioengineering 2023, 10(10), 1182; https://doi.org/10.3390/bioengineering10101182 - 12 Oct 2023
Viewed by 1216
Abstract
Articular cartilage lacks intrinsic regenerative capabilities, and the current treatments fail to regenerate damaged tissue and lead only to temporary pain relief. These limitations have prompted the development of tissue engineering approaches, including 3D culture systems. Thanks to their regenerative properties and capacity [...] Read more.
Articular cartilage lacks intrinsic regenerative capabilities, and the current treatments fail to regenerate damaged tissue and lead only to temporary pain relief. These limitations have prompted the development of tissue engineering approaches, including 3D culture systems. Thanks to their regenerative properties and capacity to recapitulate embryonic processes, spheroids obtained from mesenchymal stromal cells are increasingly studied as building blocks to obtain functional tissues. The aim of this study was to investigate the capacity of adipose stromal cells to assemble in spheroids and differentiate toward chondrogenic lineage from the perspective of cartilage repair. Spheroids were generated by two different methods (3D chips vs. Ultra-Low Attachment plates), differentiated towards chondrogenic lineage, and their properties were investigated using molecular biology analyses, biophysical measurement of mass density, weight, and size of spheroids, and confocal imaging. Overall, spheroids showed the ability to differentiate by expressing specific cartilaginous markers that correlate with their mass density, defining a critical point at which they start to mature. Considering the spheroid generation method, this pilot study suggested that spheroids obtained with chips are a promising tool for the generation of cartilage organoids that could be used for preclinical/clinical approaches, including personalized therapy. Full article
(This article belongs to the Special Issue Stem Cell-Based Technology for Personalized Medicine Solutions)
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18 pages, 5890 KiB  
Article
The Effect of Tissue Stromal Vascular Fraction as Compared to Cellular Stromal Vascular Fraction to Treat Anal Sphincter Incontinence
by Wenbin Chen, Zijian He, Shuyu Li, Zixin Wu, Jin Tan, Weifeng Yang, Guanwei Li, Xiaoling Pan, Yuying Liu, Feng-Juan Lyu and Wanglin Li
Bioengineering 2023, 10(1), 32; https://doi.org/10.3390/bioengineering10010032 - 26 Dec 2022
Viewed by 1696
Abstract
Background: The long-term prognosis of current treatments for anal sphincter incontinence (ASI) is poor. Here, we explored the efficacy of tissue adipose stromal vascular fraction SVF (tSVF) on ASI and compared it to that of cellular SVF (cSVF). We then investigated possible mechanisms. [...] Read more.
Background: The long-term prognosis of current treatments for anal sphincter incontinence (ASI) is poor. Here, we explored the efficacy of tissue adipose stromal vascular fraction SVF (tSVF) on ASI and compared it to that of cellular SVF (cSVF). We then investigated possible mechanisms. Methods: Rat cSVF and tSVF were isolated and labeled with DIL. One day after modeling, three groups received phosphate-buffered saline (PBS), cSVF, tSVF, respectively. The control group received nil modeling nor any treatments. The effect was assessed by function test for anal pressure and electromyography, and staining for fiber content, proliferation and differentiation at day 5 and day 10. Results: cSVF injection resulted in faster healing than tSVF. The cSVF group showed significant improvement on anal pressure on day 10. For the electromyography test, cSVF showed significant improvement for the frequencies on day 10, and for the peak values on both time points, while tSVF showed significant improvement for the peak values on day 10. The two SVF both alleviated fibrosis. Immunofluorescence tracing identified differentiation of some injected cells towards myosatellite cells and smooth muscle cells in both SVF groups. For all the tests, the tSVF group tends to have similar or lower effects than the cSVF group with no significant difference. Conclusion: cSVF and tSVF are both safe and effective in treating ASI, while the effect of cSVF is slighter higher than tSVF. Full article
(This article belongs to the Special Issue Stem Cell-Based Technology for Personalized Medicine Solutions)
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Review

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43 pages, 6919 KiB  
Review
Salivary Gland Bioengineering
by Stephen C. Rose, Melinda Larsen, Yubing Xie and Susan T. Sharfstein
Bioengineering 2024, 11(1), 28; https://doi.org/10.3390/bioengineering11010028 - 26 Dec 2023
Viewed by 1627
Abstract
Salivary gland dysfunction affects millions globally, and tissue engineering may provide a promising therapeutic avenue. This review delves into the current state of salivary gland tissue engineering research, starting with a study of normal salivary gland development and function. It discusses the impact [...] Read more.
Salivary gland dysfunction affects millions globally, and tissue engineering may provide a promising therapeutic avenue. This review delves into the current state of salivary gland tissue engineering research, starting with a study of normal salivary gland development and function. It discusses the impact of fibrosis and cellular senescence on salivary gland pathologies. A diverse range of cells suitable for tissue engineering including cell lines, primary salivary gland cells, and stem cells are examined. Moreover, the paper explores various supportive biomaterials and scaffold fabrication methodologies that enhance salivary gland cell survival, differentiation, and engraftment. Innovative engineering strategies for the improvement of vascularization, innervation, and engraftment of engineered salivary gland tissue, including bioprinting, microfluidic hydrogels, mesh electronics, and nanoparticles, are also evaluated. This review underscores the promising potential of this research field for the treatment of salivary gland dysfunction and suggests directions for future exploration. Full article
(This article belongs to the Special Issue Stem Cell-Based Technology for Personalized Medicine Solutions)
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