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Animals
Special Issues
Recent Advances in Rapid Detection of Animal Virus
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Recent Advances in Rapid Detection of Animal Virus

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Veterinary Clinical Studies".

Deadline for manuscript submissions: closed (31 August 2022) | Viewed by 8857

Special Issue Editors

Prof. Dr. Fei Liu

E-Mail Website
Guest Editor
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
Interests: rapid detection; single molecule technology; infection and immunity; coronavirus; RNA viruses; immunometabolism; intestinal health; protein engineering; protein drugs; vaccine
Special Issues, Collections and Topics in MDPI journals
Dr. Yi Yang

E-Mail Website
Guest Editor
College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China
Interests: molecular immunology and protein chemistry; vaccine design based on three-dimensional structure of protein; assembly of protein nanoparticles and design of multivalent chimeric vaccines; highly sensitive serological detection based on VLPs; molecular mechanisms of virus entry into cells
Dr. Zhixin Feng

E-Mail Website
Guest Editor
Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Interests: mucosal vaccine design against swine diseases and matched mucosal immune diagnostic kit or monitoring techniques; pathogenesis and mucosal immune mechanism, prevention and control strategy of animal mycoplasma diseases

Special Issue Information

Dear Colleagues, 

Thus far, there is no effective vaccine and therapeutic drugs for many animal diseases (ASF, PRRS, Brucellosis, AIV, etc.), and early monitoring is one of the most important solutions to prevent and control animal diseases. At present, TaqMan-based real-time quantitative PCR (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) are the most widely used immunological and molecular diagnostic methods in EU reference laboratories and OIE. However, the methods mentioned above require sending samples inside the farm to professional or third-party testing laboratories, which requires a longer time to obtain the testing results due to shipping and testing in the laboratories. Moreover, long-distance moving of biological samples can also lead to higher biological security risks. Therefore, it is critical to make an accurate diagnosis at the site of infection.

In recent years, many innovations in animal virus detections have been proposed, and the aim of this Special Issue is to publish original research papers or reviews concerning rapid detection of animal virus. With the help of fast and sensitive on-site detection technology, the establishment of rapid and efficient virus detection methods will not only contribute to disease prevention and control, prevent the spread and spread of the epidemic, but also save time and labor costs, which can be widely used in the field.

Areas of interest: point-of-care testing of animal virus; microfluidic chip detection technology; non-amplified nucleic acid technology; isothermal amplification nucleic acid detection technology; extractionless nucleic acid detection; wash-free immunoassay; chemiluminescent detection; miniaturization of detection devices and other related topics on rapid detection of animal virus

We invite you to share your recent findings through this Special Issue.

Dr. Fei Liu
Dr. Yi Yang
Dr. Zhixin Feng
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Animals is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • point-of-care testing
  • microfluidic chip
  • non-amplified
  • isothermal amplification
  • extractionless
  • wash-free
  • miniaturization detection
  • rapid detection
  • animal virus

Published Papers (4 papers)

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Research

10 pages, 523 KiB  
Article
FTA Cards as a Rapid Tool for Collection and Transport of Infective Samples: Experience with Foot-and-Mouth Disease Virus in Libya
by Fadila Abosrer, Giulia Pezzoni, Emiliana Brocchi, Anna Castelli, Stefano Baselli, Santina Grazioli, Hafsa Madani, Elfurgani Kraim, Abdunaser Dayhum and Ibrahim Eldaghayes
Animals 2022, 12(22), 3198; https://doi.org/10.3390/ani12223198 - 18 Nov 2022
Cited by 3 | Viewed by 1893
Abstract
Foot-and-mouth disease (FMD) is a viral disease, widespread and highly contagious, that mainly affects cloven-hoofed domestic and wild animals. FMD can lead to high economic losses due to the reduction in animal production such as a drop in milk production, loss of body [...] Read more.
Foot-and-mouth disease (FMD) is a viral disease, widespread and highly contagious, that mainly affects cloven-hoofed domestic and wild animals. FMD can lead to high economic losses due to the reduction in animal production such as a drop in milk production, loss of body weight, and a high mortality rate in young ruminants. Sixteen samples were collected from animals showing typical clinical signs of FMD during the last FMD outbreak in Libya in 2018–2019. Flinders Technology Associates (FTA) cards impressed with blood, swabs, or vesicular epithelium samples were shipped to the WOAH FMD reference laboratory in Brescia, Italy, and tested for the detection of FMD viruses. Nucleic acids were extracted from the FTA cards, and molecular testing based on real-time RT-PCR assays was carried out, of which one was specifically designed for the detection of the FMD virus of serotype O, topotype O/East Africa-3 (O/EA-3), that was further confirmed by a sequence analysis of the VP1 gene. The phylogenetic analysis of the VP1 gene showed a nucleotide identity of more than 99% between the virus circulating in Libya and the FMD virus strains isolated in Algeria in 2019. Full article
(This article belongs to the Special Issue Recent Advances in Rapid Detection of Animal Virus)
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10 pages, 1583 KiB  
Article
Visual and Rapid Detection of Porcine Epidemic Diarrhea Virus (PEDV) Using Reverse Transcription Loop-Mediated Isothermal Amplification Method
by Chunhua Li, Jieling Liang, Dan Yang, Qi Zhang, Denian Miao, Xizhong He, Yanan Du, Wanjing Zhang, Jianping Ni and Kai Zhao
Animals 2022, 12(19), 2712; https://doi.org/10.3390/ani12192712 - 09 Oct 2022
Cited by 6 | Viewed by 1964
Abstract
Porcine epidemic diarrhea virus (PEDV) can cause severe infectious porcine epidemic diarrhea (PED) and infect different ages of pigs, resulting in sickness and death among suckling pigs. For PEDV detection, finding an effective and rapid method is a priority. In this study, we [...] Read more.
Porcine epidemic diarrhea virus (PEDV) can cause severe infectious porcine epidemic diarrhea (PED) and infect different ages of pigs, resulting in sickness and death among suckling pigs. For PEDV detection, finding an effective and rapid method is a priority. In this study, we established an effective reverse transcription loop-mediated isothermal amplification (RT-LAMP) method for PEDV detection. Three sets of primers, specific for eight different sequences of the PEDV N gene, were designed in this study. The optimized RT-LAMP amplification program was as follows: 59 min at 61.9 °C and 3 min at 80 °C. The RT-LAMP results were confirmed with the addition of SYBR Green I fluorescence dye and with the detection of a ladder-like band by conventional gel electrophoresis analysis, which demonstrated a significant agreement between the two methods. The LOD of PEDV by RT-LAMP was 0.0001 ng/μL. Compared with RT-LAMP, the traditional RT-PCR method is 100-fold less sensitive. The RT-LAMP results had no cross-reaction with porcine parvovirus (PPV), porcine circovirus type 1 (PCV1), porcine pseudorabies virus (PRV), porcine circovirus type 2 (PCV2), rotavirus (RV), transmissible gastroenteritis virus (TGEV) and porcine reproductive and respiratory syndrome virus (PRRSV). Consequently, the newly developed RT-LAMP method could provide an accurate and reliable tool for PEDV diagnosis. Full article
(This article belongs to the Special Issue Recent Advances in Rapid Detection of Animal Virus)
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12 pages, 1995 KiB  
Article
LAMP Coupled CRISPR-Cas12a Module for Rapid, Sensitive and Visual Detection of Porcine Circovirus 2
by Lei Lei, Fan Liao, Lei Tan, Deyong Duan, Yang Zhan, Naidong Wang, Yuge Wang, Xiaoye Peng, Kaixin Wang, Xiaojiu Huang, Yi Yang and Aibing Wang
Animals 2022, 12(18), 2413; https://doi.org/10.3390/ani12182413 - 14 Sep 2022
Cited by 9 | Viewed by 2253
Abstract
Porcine circovirus 2 (PCV2) is the main pathogen of porcine circovirus-associated disease (PCVAD), which can cause considerable economic loss to the pig industry. The diagnosis of PCVAD is complicated and requires a series of clinical, pathological, and virological methods. Therefore, a rapid, highly [...] Read more.
Porcine circovirus 2 (PCV2) is the main pathogen of porcine circovirus-associated disease (PCVAD), which can cause considerable economic loss to the pig industry. The diagnosis of PCVAD is complicated and requires a series of clinical, pathological, and virological methods. Therefore, a rapid, highly sensitive, on-site, and visual diagnostic approach would facilitate dealing with the spread of PCV2. In this study, we intended to establish a new and effective PCV2 detection method through combining the no specific equipment requirement advantage of loop-mediated isothermal amplification (LAMP) with the property of clustered regular interspaced short palindromic repeats (CRISPR)/Cas12a system possessing the huLbCas12a collateral cleavage activity able to cleave single-stranded DNA fluorophore quencher probe sensor (designed as LAPM-CRISPR). Following a series of optimizations of its reaction conditions, this LAMP-CRISPR-based PCV2 detection could be conducted in constant temperature equipment, with the result reflected in a direct visual readout way. This established PCV2 detection approach presented fine sensitivity, rapidity, specificity, and reliability, as demonstrated by a low detectable limit of 1 copy/μL, completed within an hour, no cross-reaction with main porcine DNA or RNA viruses like PCV1, PCV3, and PEDV, and a 100% coincidence rate with that of the quantitative PCR (qPCR) method in the evaluation of 30 clinical blood samples, respectively. Therefore, this novel method makes rapid, on-site, visual, highly sensitive, and specific detection of PCV2 possible, facilitating the prevention of this pathogen in the field. Full article
(This article belongs to the Special Issue Recent Advances in Rapid Detection of Animal Virus)
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11 pages, 818 KiB  
Article
Rapid Nucleic Acid Extraction for Aquatic Animal DNA Virus Determination Using Chelex 100 Resin via Conventional PCR and Digital Droplet PCR Detection
by Xi Hu, Nan Jiang, Yiqun Li, Yong Zhou, Yuding Fan, Mingyang Xue, Lingbing Zeng, Wenzhi Liu and Yan Meng
Animals 2022, 12(15), 1999; https://doi.org/10.3390/ani12151999 - 08 Aug 2022
Cited by 2 | Viewed by 2129
Abstract
Molecular diagnostic testing for viral pathogens is crucial in aquaculture. The efficient and convenient preparation of pathogenic microbial nucleic acids is the basis of molecular diagnosis. Here, we developed a simplified deoxyribonucleic acid (DNA) extraction method from aquatic animal DNA viruses using the [...] Read more.
Molecular diagnostic testing for viral pathogens is crucial in aquaculture. The efficient and convenient preparation of pathogenic microbial nucleic acids is the basis of molecular diagnosis. Here, we developed a simplified deoxyribonucleic acid (DNA) extraction method from aquatic animal DNA viruses using the Chelex 100 resin. The nucleic acid was extracted from infected tissues and cell culture for the detection of three common aquatic viral pathogens (CEV, CyHV-2, and GSIV). We compared the extraction effects of a current commercial kit extraction method and the Chelex 100 resin extraction method according to nucleic acid concentration, conventional polymerase chain reaction (PCR), and digital droplet PCR (ddPCR). The results indicated that both extraction procedures could obtain high-quality nucleotide samples. Extracting DNA using the Chelex 100 resin led to better detective efficiency for ddPCR molecular diagnostic testing. The whole process took less than 20 min, and only Chelex 100 resin solution was added to the tissues or cells without multiple tubes being transferred several times. The extracted DNA concentration and the detection sensitivity were high. These results indicated that the Chelex 100 resin solution has the advantages of speed, efficiency, and economy compared to the commercial kit. In addition, the higher pH value (10–11) of the Chelex 100 resin solution markedly improved the detection sensitivity compared to a lower pH value (9–10). In conclusion, the comparison of the Chelex 100 Resin and commercial viral DNA extraction kits revealed the good performance of the Chelex 100 resin solution at pH 10–11 in DNA extraction for PCR amplification from aquatic animal viral samples of tissues and cells in molecular diagnostic testing. It is both rapid and cost-effective. Full article
(This article belongs to the Special Issue Recent Advances in Rapid Detection of Animal Virus)
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