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13 pages, 739 KiB

Open AccessArticle

Potential New Therapeutic Approaches Based on Punica granatum Fruits Compared to Synthetic Anthelmintics for the Sustainable Control of Gastrointestinal Nematodes in Sheep

by Fabio Castagna, Roberto Bava, Vincenzo Musolino, Cristian Piras, Antonio Cardamone, Cristina Carresi, Carmine Lupia, Antonio Bosco, Laura Rinaldi, Giuseppe Cringoli, Ernesto Palma, Vincenzo Musella and Domenico Britti

Animals 2022, 12(20), 2883; https://doi.org/10.3390/ani12202883 - 21 Oct 2022

Cited by 12 | Viewed by 1541

Abstract

Parasites, in particular, gastrointestinal nematodes (GINs) represent one of the main burdens affecting small ruminant farming and pose a serious threat to their health, welfare, productivity, and reproduction. The correct management of animals and the correct use of anthelmintic drugs are the pillars [...] Read more.

Parasites, in particular, gastrointestinal nematodes (GINs) represent one of the main burdens affecting small ruminant farming and pose a serious threat to their health, welfare, productivity, and reproduction. The correct management of animals and the correct use of anthelmintic drugs are the pillars of the GIN control programs for small ruminants. However, globally due to the indiscriminate use of synthetic anthelmintics, there is a significant increase in anthelmintic resistance phenomena to one or more classes of drugs. Even if such a problem never represented a serious threat in southern Italy because of the favourable environmental conditions and because of the good farm management, the phenomenon is actually showing a steep increasing trend and requires alternative treatment measures and constant monitoring. The use of phytotherapies is considered a valuable alternative approach for GIN control in small ruminants and could help with reducing the amount of synthetic drugs used and the forthcoming anthelmintic resistance. From this perspective, the Calabria territory offers a wide number of plants with anthelmintic efficacy that could be helpful for this purpose. The aim of this study was to evaluate the anthelmintic efficacy of aqueous pomegranate (Punica granatum L.) macerate compared to the treatment with Ivermectin and Albendazole in sheep naturally infected with GINs. The pomegranate macerate derives from the ethnoveterinary knowledge of the Calabria region, Southern Italy. The anthelmintic efficacy was evaluated according to the faecal egg count reduction test (FECRt) using the FLOTAC techniques in two sheep farms in Southern Italy. The FECR was calculated from individual samples using the formula FECR = 100 × (1 − [T2/C2]). The treatment with Albendazole in the first farm showed an efficacy of 99.8% after 14 days and 94.8% after 21 days, while the treatment with Ivermectin in the second farm showed an efficacy of 99.9% after 14 days and 96.5% after 21 days of treatment. The pomegranate macerate, in both farms, showed a value of efficacy of around 50% from day 7 to day 21 after the treatment. Previous studies highlighted the presence of gallic acid as the main component in the pomegranate macerate, and its efficacy in nematode control has been as well previously demonstrated in other plant extracts. This in vivo study demonstrated the unequivocal efficacy of plant macerate in easily reducing 50% of the number of GIN eggs in sheep faeces. These results, obtained without the use of synthetic anthelmintics, indicate the use of green veterinary pharmacology as a sustainable alternative to the use of synthetic drugs to reduce the increase in drug resistance phenomena and the environmental impact. Full article

(This article belongs to the Special Issue Nutraceuticals, Phytonutrients, and Phytotherapy for Improvement of Animal Health and Welfare)

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12 pages, 897 KiB

Open AccessArticle

Effect of Aqueous Extract of Maca Addition to an Extender for Chilled Canine Semen

by Chiara Del Prete, Alfonso Calabria, Valentina Longobardi, Veronica Palumbo, Barbara Merlo, Eleonora Iacono, Simona Tafuri, Domenico Carotenuto, Francesca Ciani, Sara Damiano, Roberto Ciarcia and Natascia Cocchia

Animals 2022, 12(13), 1638; https://doi.org/10.3390/ani12131638 - 26 Jun 2022

Cited by 5 | Viewed by 1847

Abstract

Antioxidant supplementation has been proposed as a new strategy to improve the long-term preservation of semen. The aim of this study was to evaluate the effect of Maca supplementation of semen extender on quality-related canine semen parameters during cooling. Ejaculates from nine dogs [...] Read more.

Antioxidant supplementation has been proposed as a new strategy to improve the long-term preservation of semen. The aim of this study was to evaluate the effect of Maca supplementation of semen extender on quality-related canine semen parameters during cooling. Ejaculates from nine dogs were cooled for 7 days in the absence (control group) or in the presence of 10, 20 and 50 μL/mL of an aqueous extract of Maca. Sperm were evaluated for sperm viability, motility, DNA fragmentation and lipid peroxidation after 3 h, 24 h, 4 days and 7 days of storage. The addition of 10 μL/mL of Maca preserved sperm DNA and plasma membrane integrity at 3 h and increased sperm curvilinear velocity after 24 h. Treatment with 20 and 50 μL/mL of Maca increased the percentage of hyperactivated sperm after 3 h. Moreover, semen treated with 20 μL/mL of Maca decreased lipid peroxidation at 24 h. A significant reduction of sperm DNA and plasma membrane integrity as well as of kinetics parameters between 3 and 24 h of refrigerated storage with the higher concentration tested was observed. Although Maca was not able to protect canine semen with extended refrigeration storage time, it increased hyperactivation and preserved DNA integrity in short-term storage. Full article

(This article belongs to the Special Issue Nutraceuticals, Phytonutrients, and Phytotherapy for Improvement of Animal Health and Welfare)

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15 pages, 4451 KiB

Open AccessArticle

Ethanol Extract of Artemisia Annua Prevents LPS-Induced Inflammation and Blood–Milk Barrier Disruption in Bovine Mammary Epithelial Cells

by Jie Song, Yao Hu, Lifang Wang and Changjin Ao

Animals 2022, 12(10), 1228; https://doi.org/10.3390/ani12101228 - 10 May 2022

Cited by 9 | Viewed by 1928

Abstract

This experiment evaluated the pre-protective effect of AAE on inflammatory injury and tight junction disturbance in bMECs induced by LPS. The bMECs were treated with AAE (3, 6, 12 μg/mL) for 3 h and then incubated with 10 μg/mL lipopolysaccharide (LPS) for 12 [...] Read more.

This experiment evaluated the pre-protective effect of AAE on inflammatory injury and tight junction disturbance in bMECs induced by LPS. The bMECs were treated with AAE (3, 6, 12 μg/mL) for 3 h and then incubated with 10 μg/mL lipopolysaccharide (LPS) for 12 h. Our results showed that LPS significantly increased the mRNA and protein expression of CD36, induced the phosphorylation of IκBα and p65 and elevated the levels of TNF-α, IL-1β and IL-6 mRNA, which further resulted in ultrastructural damage, disrupted the expression of tight junction proteins (occludin, zonula occludens (ZO-1) and claudin-1) and decreased the viability of bMECs (p < 0.05). More importantly, AAE pretreatment attenuated the expression of CD36, suppressed the activity of the NF-κB signaling pathway and down-regulated the levels of inflammatory factors in LPS-stimulated bMECs (p < 0.05). Therefore, AAE can effectively protect bMECs against inflammatory injury and tight junction dysfunction, which has important research value for the prevention of bovine mastitis. Full article

(This article belongs to the Special Issue Nutraceuticals, Phytonutrients, and Phytotherapy for Improvement of Animal Health and Welfare)

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17 pages, 673 KiB

Open AccessArticle

Productive Performance, Ovarian Follicular Development, Lipid Peroxidation, Antioxidative Status, and Egg Quality in Laying Hens Fed Diets Supplemented with Salvia officinalis and Origanum majorana Powder Levels

by Ahmed A. Saleh, Shimaa Hamed, Aziza M. Hassan, Khairy Amber, Wael Awad, Mohammed H. Alzawqari and Mustafa Shukry

Animals 2021, 11(12), 3513; https://doi.org/10.3390/ani11123513 - 09 Dec 2021

Cited by 9 | Viewed by 3177

Abstract

This study aimed to assess the effect of dietary supplementation with different levels of Salvia officinalis and/or Origanum majorana on productive performance, ovarian follicular development, lipid peroxidation, antioxidative status, and egg quality in laying hens. Two hundred and ninety-four 45-week-old Bovans brown hens [...] Read more.

This study aimed to assess the effect of dietary supplementation with different levels of Salvia officinalis and/or Origanum majorana on productive performance, ovarian follicular development, lipid peroxidation, antioxidative status, and egg quality in laying hens. Two hundred and ninety-four 45-week-old Bovans brown hens were allocated into seven groups, with seven replicates of six hens each. The first group was fed with the basal considered as a control (A); the second (B) and third (C) groups were provided with the same control diet further supplemented with 0.5 and 1 kg/ton Salvia officinalis, respectively; the fourth (D) and fifth (E) groups received the control diet further supplemented with 0.5 and 1 kg/ton Origanum majorana, respectively; while the sixth (F) and the seventh (G) groups were offered a diet supplemented with 0.5 kg/ton Salvia officinalis and 0.5 kg/ton Origanum majorana and 1 kg/ton Salvia officinalis and 1 kg/ton Origanum majorana, respectively. No significant effects were observed in the final body weight (BW) and feed intake (FI) of the laying hens. In the diets supplemented with Salvia officinalis and Origanum majorana, the egg weights for groups C, F, and G had significantly higher values only compared to group D. The supplementation of the diets with Salvia officinalis and/or Origanum majorana significantly (p < 0.05) increased the Follicle stimulating hormone (FSH), luteinizing hormone (LH), and estradiol estrogenic hormone concentration, except for Origanum majorana at both levels with regard to estradiol. The dietary utilization of Salvia officinalis and Origanum majorana did not significantly alter the plasma glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT), total protein, albumin, globulin, and High density lipoprotein (HDL) parameters. Cholesterol, glucose, triglyceride, and Low density lipoprotein (LDL) were decreased (p < 0.05) in the birds fed with Salvia officinalis and/or Origanum majorana supplemented diets. Moreover, at both doses, the dietary supplementation with Salvia officinalis and Origanum majorana decreased (p < 0.05) the yolk cholesterol and liver Malondialdehyde (MDA) levels. In addition, the dietary enrichment with Salvia officinalis and/or Origanum majorana decreased (p < 0.05) the palmitoleic and stearic fatty acids’ egg yolk concentration. In contrast, the yolk linoleic fatty acid concentration was significantly increased by Salvia officinalis and/or Origanum majorana. In conclusion, dietary supplementation with Salvia officinalis and/or Origanum positively affected productive performance, ovarian follicular development, antioxidant activity, hormonal status, and steroidogenesis in Bovans brown laying hens. Full article

(This article belongs to the Special Issue Nutraceuticals, Phytonutrients, and Phytotherapy for Improvement of Animal Health and Welfare)

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12 pages, 600 KiB

Open AccessArticle

Dietary Supplementation of Olive Mill Waste Water Polyphenols in Rabbits: Evaluation of the Potential Effects on Hepatic Apoptosis, Inflammation and Metabolism through RT-qPCR Approach

by Katia Cappelli, Flavia Ferlisi, Samanta Mecocci, Margherita Maranesi, Massimo Trabalza-Marinucci, Massimo Zerani, Alessandro Dal Bosco and Gabriele Acuti

Animals 2021, 11(10), 2932; https://doi.org/10.3390/ani11102932 - 11 Oct 2021

Cited by 9 | Viewed by 2193

Abstract

Agro-industrial processing for the production of food or non-food products generates a wide range of by-products and residues rich in bioactive compounds including polyphenols. The concentration of these by-products is sometimes higher than in the original raw material as in the case of [...] Read more.

Agro-industrial processing for the production of food or non-food products generates a wide range of by-products and residues rich in bioactive compounds including polyphenols. The concentration of these by-products is sometimes higher than in the original raw material as in the case of olive mill waste water (OMWW), one of the main by-products of olive oil extraction. Polyphenols are secondary plant metabolites that regulate the expression of specific inflammatory genes, transcriptional factors and pro/anti-apoptotic molecules, thus modulating the signaling pathways essential for cell health and homeostasis. The liver plays a key role in regulating homeostasis by responding to dietary changes in order to maintain nutritional and physiological states. In this study a nutrigenomic approach was adopted, which focuses on the effects of diet–health–gene interactions and the modulation of cellular processes, in order to evaluate the expression of the genes (AGER, BAX, COX2, IL1B, PPARA, PPARG, SIRT1, TNFA) involved in these interactions in the livers of rabbits fed with a diet supplemented with OMWW (POL) or without supplements (control, CTR). The RT-qPCR analysis showed the down-regulation of SIRT1, TNFA, AGER, BAX and PPARA transcripts in the POL group compared to the CTR group. These results show that OMWW dietary supplementation prevents cell death and tissue deterioration in rabbits. Full article

(This article belongs to the Special Issue Nutraceuticals, Phytonutrients, and Phytotherapy for Improvement of Animal Health and Welfare)

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14 pages, 2125 KiB

Open AccessArticle

Effects of Dietary Polyphenols from Olive Mill Waste Waters on Inflammatory and Apoptotic Effectors in Rabbit Ovary

by Margherita Maranesi, Cecilia Dall’Aglio, Gabriele Acuti, Katia Cappelli, Massimo Trabalza Marinucci, Roberta Galarini, Chiara Suvieri and Massimo Zerani

Animals 2021, 11(6), 1727; https://doi.org/10.3390/ani11061727 - 09 Jun 2021

Cited by 9 | Viewed by 4000

Abstract

The aim of this study was to evaluate the effect of dietary polyphenols on the expression of the effectors involved in inflammation and apoptosis in rabbit ovary. New Zealand White female rabbits were fed a basal control diet (CTR), or the same diet [...] Read more.

The aim of this study was to evaluate the effect of dietary polyphenols on the expression of the effectors involved in inflammation and apoptosis in rabbit ovary. New Zealand White female rabbits were fed a basal control diet (CTR), or the same diet supplemented with a polyphenolic concentrate (POL, 282.4 mg/kg) obtained from olive mill waste waters. The follicle counts and the relative mRNA (RT-qPCR) and protein (immunohistochemistry) expression of the effectors involved in inflammation (cyclooxygenase-2; interleukin-1beta; tumor necrosis factor-alpha, TNFA) and apoptosis (BCL2-associated X protein, BAX), detected in the ovaries of both groups, were examined. The POL diet increased the primary and total follicles number. Cyclooxygenase-2 gene expression was higher (p < 0.05) in the POL group than in the CTR group, whereas BAX was lower (p < 0.05) in POL than CTR. Immunohistochemistry revealed the presence of all the proteins examined, with weaker (p < 0.05) COX2 and BAX signals in POL. No differences between the CTR and POL groups were observed for IL1B and TNFA gene and protein expression. These preliminary findings show that dietary polyphenols modulate inflammatory and apoptotic activities in rabbit ovary, regulating cyclooxygenase-2 and BAX expression, thus suggesting a functional involvement of these dietary compounds in mammalian reproduction. Full article

(This article belongs to the Special Issue Nutraceuticals, Phytonutrients, and Phytotherapy for Improvement of Animal Health and Welfare)

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11 pages, 518 KiB

Open AccessArticle

Dietary Pomegranate By-Product Alleviated the Oxidative Stress Induced by Dexamethasone in Laying Hens in the Pre-Peak Period

by Yahya Eid, Abeer A Kirrella, Ahmed Tolba, Maged El-Deeb, Samy Sayed, Hanan B. El-Sawy, Mustafa Shukry and Mahmoud A. O. Dawood

Animals 2021, 11(4), 1022; https://doi.org/10.3390/ani11041022 - 05 Apr 2021

Cited by 17 | Viewed by 2155

Abstract

This experiment was conducted to assess the inclusion of the by-products of pomegranate peels to ameliorate the harmful impacts of oxidative stress in the pre-peak period of laying hens. For this, 120 local Egyptian strain hens (Inshas hens) aged 24 weeks old were [...] Read more.

This experiment was conducted to assess the inclusion of the by-products of pomegranate peels to ameliorate the harmful impacts of oxidative stress in the pre-peak period of laying hens. For this, 120 local Egyptian strain hens (Inshas hens) aged 24 weeks old were used in four treatments. Pomegranate peel powder was included at 2% and 4% in the diets of laying hens subjected to oxidative injuries induced by dexamethasone compared with negative and positive control groups for 12 weeks. The addition of pomegranate peel powder (PPP) reduced the adverse effects of oxidative stress induced by dexamethasone on body weight (p = 0.006) and egg production (p = 0.010) comparing to the positive control. Additionally, pomegranate peel powder had a significant positive lowering effect on plasma cholesterol (p < 0.001) and triglyceride contents (p = 0.005) compared to control groups. The lipid peroxidation indicators (MDA) were reduced, but the antioxidative enzymes (SOD, CAT, and GPx) and total antioxidant blood capacity were improved with PPP. Based on the obtained data, the present research recommends using dietary PPP up to 4% to mitigate adverse oxidative stress effects in the pre-peak laying period and as a sustainable and economical approach for agricultural development. Full article

(This article belongs to the Special Issue Nutraceuticals, Phytonutrients, and Phytotherapy for Improvement of Animal Health and Welfare)

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20 pages, 5425 KiB

Open AccessArticle

Analysis of the Productivity, Immunity, and Health Performance of Nile Tilapia (Oreochromis niloticus) Broodstock-fed Dietary Fermented Extracts Sourced from Saccharomyces cerevisiae (Hilyses): A Field Trial

by Nermeen M. Abu-Elala, Tamer El-Sayed Ali, Naela M. Ragaa, Sara E. Ali, Reham M. Abd-Elsalam, Nehal A. Younis, Dalia A. Abdel-Moneam, Aya H. Hamdien, Melina Bonato and Mahmoud A.O. Dawood

Animals 2021, 11(3), 815; https://doi.org/10.3390/ani11030815 - 14 Mar 2021

Cited by 12 | Viewed by 2979

Abstract

The present study aimed to evaluate the effect of dietary fermented extracts sourced from Saccharomyces cerevisiae (nucleotides, β-glucans and MOS) (Hilyses^®) on the production and health of Nile tilapia (Oreochromis niloticus) broodstock, as well as on seed survival and [...] Read more.

The present study aimed to evaluate the effect of dietary fermented extracts sourced from Saccharomyces cerevisiae (nucleotides, β-glucans and MOS) (Hilyses^®) on the production and health of Nile tilapia (Oreochromis niloticus) broodstock, as well as on seed survival and performance. The trial was performed in a hatchery along the spawning season and continued in the laboratory to monitor the performance in fry and fingerlings. The broodstock were divided into two groups, (C) fed a basal diet and (H) fed 0.4% Hilyses. Blood and histological parameters, antioxidant power, cortisol level and the expression of some immune-related (TLR-2, IL-1β and TNF-α) and growth-related genes (MUC-2 and IGF-1) were measured. The obtained seeds were subdivided into four treatments: (C-C) fed a basal diet, (C-H) fed 0.4% Hilyses, (H-C) fed a basal diet and (H-H) fed 0.4% Hilyses. Results revealed that the dietary inclusion of Hilyses in the broodstock increased seed production, survival, hematological parameters, and antioxidant power. Moreover, it improved the intestinal microstructure and upregulated the immune- and growth-related genes. The growth indices of fry and fingerlings were significantly increased in all Hilyses-treated groups (p < 0.05). The performance in the (H-H) group significantly surpassed those of all groups. Therefore, dietary fermented yeast could be used as a strategic solution to sustain tilapia production. Full article

(This article belongs to the Special Issue Nutraceuticals, Phytonutrients, and Phytotherapy for Improvement of Animal Health and Welfare)

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Review

Jump to: Research

21 pages, 1373 KiB

Open AccessReview

Hemp in Animal Diets—Cannabidiol

by Sepideh Fallahi, Łukasz Bobak and Sebastian Opaliński

Animals 2022, 12(19), 2541; https://doi.org/10.3390/ani12192541 - 22 Sep 2022

Cited by 9 | Viewed by 3624

Abstract

In recent years, interest in hemp use has grown owing to its chemical and medicinal properties. Several parts of this plant, such as seeds, leaves, flowers, and stems are used in medicine, industry, and environmental preservation. Although there were legal restrictions on hemp [...] Read more.

In recent years, interest in hemp use has grown owing to its chemical and medicinal properties. Several parts of this plant, such as seeds, leaves, flowers, and stems are used in medicine, industry, and environmental preservation. Although there were legal restrictions on hemp exploitation in some countries due to the trace presence of THC as a psychoactive element, many countries have legalized it in recent years. Cannabidiol or CBD is a non-psychoactive phytocannabinoid that can activate the endocannabinoid system and its receptors in the central and peripheral nervous system in bodies of different species. Cannabidiol has anti-inflammatory, antioxidative, analgesic, and anti-depressant effects. This review investigates various aspects of cannabidiol use and its potential in animals and humans. Full article

(This article belongs to the Special Issue Nutraceuticals, Phytonutrients, and Phytotherapy for Improvement of Animal Health and Welfare)

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21 pages, 751 KiB

Open AccessReview

The Role of Nutraceuticals and Phytonutrients in Chickens’ Gastrointestinal Diseases

by Lucia Biagini, Livio Galosi, Alessandra Roncarati, Anna-Rita Attili, Sara Mangiaterra and Giacomo Rossi

Animals 2022, 12(7), 892; https://doi.org/10.3390/ani12070892 - 31 Mar 2022

Cited by 6 | Viewed by 3027

Abstract

In poultry, severe gastrointestinal diseases are caused by bacteria and coccidia, with important economic losses in the poultry industry and requirement of treatments which, for years, were based on the use of antibiotics and chemotherapies. Furthermore, Salmonella spp., Clostridium perfringens, and Campylobacter [...] Read more.

In poultry, severe gastrointestinal diseases are caused by bacteria and coccidia, with important economic losses in the poultry industry and requirement of treatments which, for years, were based on the use of antibiotics and chemotherapies. Furthermore, Salmonella spp., Clostridium perfringens, and Campylobacter jejuni can cause serious foodborne diseases in people, resulting from consumption of poultry meat, eggs, and derived products. With the spread of antibiotic resistance, which affects both animals and humans, the restriction of antibiotic use in livestock production and the identification of a list of “critically important antimicrobials” became necessary. For this reason, researchers focused on natural compounds and effective alternatives to prevent gastrointestinal disease in poultry. This review summarizes the results of several studies published in the last decade, describing the use of different nutraceutical or phytonutrients in poultry industry. The results of the use of these products are not always encouraging. While some of the alternatives have proven to be very promising, further studies will be needed to verify the efficacy and practical applicability of other compounds. Full article

(This article belongs to the Special Issue Nutraceuticals, Phytonutrients, and Phytotherapy for Improvement of Animal Health and Welfare)

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