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17 pages, 1580 KiB

Open AccessArticle

Olive Mill Waste-Water Extract Enriched in Hydroxytyrosol and Tyrosol Modulates Host–Pathogen Interaction in IPEC-J2 Cells

by Flavia Ferlisi, Chiara Grazia De Ciucis, Massimo Trabalza-Marinucci, Floriana Fruscione, Samanta Mecocci, Giulia Franzoni, Susanna Zinellu, Roberta Galarini, Elisabetta Razzuoli and Katia Cappelli

Animals 2024, 14(4), 564; https://doi.org/10.3390/ani14040564 - 07 Feb 2024

Viewed by 882

Abstract

The dietary supplementation of olive oil by-products, including olive mill waste-water (OMWW) in animal diets, is a novel application that allows for their re-utilization and recycling and could potentially decrease the use of antibiotics, antimicrobial resistance risk in livestock species, and the occurrence [...] Read more.

The dietary supplementation of olive oil by-products, including olive mill waste-water (OMWW) in animal diets, is a novel application that allows for their re-utilization and recycling and could potentially decrease the use of antibiotics, antimicrobial resistance risk in livestock species, and the occurrence of intestinal diseases. Salmonella serovar typhimurium is one of the most widespread intestinal pathogens in the world, causing enterocolitis in pigs. The aim of this study was to investigate the effect of an OMWW extract enriched in polyphenols (hydroxytyrosol and tyrosol) in the immune response of an intestinal porcine epithelial cell line (IPEC-J2) following S. typhimurium infection. Cells were pre-treated with OMWW-extract polyphenols (OMWW-EP, 0.35 and 1.4 µg) for 24 h and then infected with S. typhimurium for 1 h. We evaluated bacterial invasiveness and assayed IPEC-J2 gene expression with RT-qPCR and cytokine release with an ELISA test. The obtained results showed that OMWW-EP (1.4 µg) significantly reduced S. typhimurium invasiveness; 0.35 µg decreased the IPEC-J2 gene expression of IL1B, MYD88, DEFB1 and DEFB4A, while 1.4 µg down-regulated IL1B and DEFB4A and increased TGFB1. The cytokine content was unchanged in infected cells. This is the first study demonstrating the in vitro immunomodulatory and antimicrobial activity of OMWW extracts enriched in polyphenols, suggesting a protective role of OMWW polyphenols on the pig intestine and their potential application as feed supplements in farm animals such as pigs. Full article

(This article belongs to the Special Issue Antioxidants, Nutraceuticals, and Functional Food in Animals)

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11 pages, 228 KiB

Open AccessArticle

Effects of Complex Antioxidants Added to Chicken Diet on Growth Performance, Serum Biochemical Indices, Meat Quality, and Antioxidant Capacity

by Xiaochun Chen, Dan Zeng, Xiangyi Zeng and Qiufeng Zeng

Animals 2024, 14(3), 360; https://doi.org/10.3390/ani14030360 - 23 Jan 2024

Viewed by 805

Abstract

This study aimed to evaluate the effects of diets supplemented with various levels of complex antioxidants (CA) containing tertiary butylhydroquinone (TBHQ) and tea polyphenols (TP) on growth performance, meat quality of breast and leg muscles, serum biochemistry, [...] Read more.

This study aimed to evaluate the effects of diets supplemented with various levels of complex antioxidants (CA) containing tertiary butylhydroquinone (TBHQ) and tea polyphenols (TP) on growth performance, meat quality of breast and leg muscles, serum biochemistry, and antioxidant capacity of serum, liver, breast meat, jejunum, and ileum in broilers. A total of 600 one-day-old Arbor Acres male broilers with similar body weights were randomly divided into three groups (10 replicates/group, 20 broilers/replicate). Birds in the three experimental groups were fed a basal diet with CA at 0, 300, and 500 mg/kg. The results showed that supplementing with 300 mg/kg CA significantly increased (p < 0.05) 42 d BW and 22–42 d ADG, and markedly decreased (p < 0.05) 22–42 d F: G ratio in comparison to the control group. Birds fed a diet with 300 mg/kg CA had a higher (p < 0.05) pH of chicken meat at 24 h and 48 h post mortem and lower (p < 0.05) yellowness values (b*) of chicken meat at 45 min and 24 h post mortem, along with a lower (p < 0.05) cooking loss. Supplementing with 300 mg/kg CA significantly increased (p < 0.05) serum and liver T-SOD activity, serum T-AOC level, as well as jejunual GST activity, and significantly decreased (p < 0.05) liver MDA content when compared with the control group. These results indicate that diet supplementation with 300 mg/kg CA containing TBHQ and TP could improve growth performance and meat quality by increasing the antioxidant capacity of broilers. Full article

(This article belongs to the Special Issue Antioxidants, Nutraceuticals, and Functional Food in Animals)

10 pages, 748 KiB

Open AccessArticle

Beta-Carotene Supplementation for Sows: Reproductive Parameters and Productive Performance of Piglets

by Amanda Medeiros Araújo de Oliveira, Ednardo Rodrigues Freitas, Denise Batista Nogueira, Gabriel Gobira de Alcântara Araújo, Lucas Melo de Silva, Eduardo Afonso Frandi Butolo, Kassia Moreira Santos, Maurício Frias Prata, Claudia Cassimira da Silva Martins, Adsos Adami Passos, Carlos Lozano, Leonardo Augusto Fonseca Pascoal and Pedro Henrique Watanabe

Animals 2023, 13(23), 3730; https://doi.org/10.3390/ani13233730 - 01 Dec 2023

Viewed by 951

Abstract

The rapid fetal development and the increased demand for milk result in a catabolic state and oxidative stress in hyperprolific sows. Despite animal defense mechanisms, the dietary supplementation of antioxidants is being evaluated to reduce the impacts of excess free radicals. The aim [...] Read more.

The rapid fetal development and the increased demand for milk result in a catabolic state and oxidative stress in hyperprolific sows. Despite animal defense mechanisms, the dietary supplementation of antioxidants is being evaluated to reduce the impacts of excess free radicals. The aim of this study was to evaluate the short- and long-term effects of beta-carotene supplementation for sows on the reproductive response and performance of suckling piglets. A total of 120 sows were distributed in a 3 × 4 factorial arrangement of three supplementation levels (B0–no supplementation, B200–200 mg beta-carotene/day and B400–400 mg beta-carotene/day) and four groups of parity order (1st, 2nd, 3rd, above 4th). Beta-carotene supplementation during lactation resulted in a higher litter weight at weaning. A higher average weight of piglets and litter weight at birth were observed, as well as a greater number of piglets weaned and litter weight at weaning in sows supplemented with 400 mg of beta-carotene during gestation and lactation periods. Supplementation with 200 and 400 mg of beta-carotene resulted in a greater weight at weaning and daily weight gain in piglets. Daily supplementation with 400 mg of beta-carotene in the prepartum and lactation phases provides a greater litter weight at weaning and, when supplemented in the pre-gestation and gestation periods, results in a greater litter weight at birth and at weaning. Full article

(This article belongs to the Special Issue Antioxidants, Nutraceuticals, and Functional Food in Animals)

20 pages, 2344 KiB

Open AccessArticle

Effect of Red-Beetroot-Supplemented Diet on Gut Microbiota Composition and Metabolite Profile of Weaned Pigs—A Pilot Study

by Opeyemi O. Adekolurejo, Katie McDermott, Henry M. R. Greathead, Helen M. Miller, Alan R. Mackie and Christine Boesch

Animals 2023, 13(13), 2196; https://doi.org/10.3390/ani13132196 - 04 Jul 2023

Cited by 1 | Viewed by 1245

Abstract

Red beetroot is a well-recognized and established source of bioactive compounds (e.g., betalains and polyphenols) with anti-inflammatory and antimicrobial properties. It is proposed as a potential alternative to zinc oxide with a focus on gut microbiota modulation and metabolite production. In this study, [...] Read more.

Red beetroot is a well-recognized and established source of bioactive compounds (e.g., betalains and polyphenols) with anti-inflammatory and antimicrobial properties. It is proposed as a potential alternative to zinc oxide with a focus on gut microbiota modulation and metabolite production. In this study, weaned pigs aged 28 days were fed either a control diet, a diet supplemented with zinc oxide (3000 mg/kg), or 2% and 4% pulverized whole red beetroot (CON, ZNO, RB2, and RB4; respectively) for 14 days. After pigs were euthanized, blood and digesta samples were collected for microbial composition and metabolite analyses. The results showed that the diet supplemented with red beetroot at 2% improved the gut microbial richness relative to other diets but marginally influenced the cecal microbial diversity compared to a zinc-oxide-supplemented diet. A further increase in red beetroot levels (4%—RB4) led to loss in cecal diversity and decreased short chain fatty acids and secondary bile acid concentrations. Also, an increased Proteobacteria abundance, presumably due to increased lactate/lactic-acid-producing bacteria was observed. In summary, red beetroot contains several components conceived to improve the gut microbiota and metabolite output of weaned pigs. Future studies investigating individual components of red beetroot will better elucidate their contributions to gut microbiota modulation and pig health. Full article

(This article belongs to the Special Issue Antioxidants, Nutraceuticals, and Functional Food in Animals)

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18 pages, 805 KiB

Open AccessArticle

Pequi Oil (Caryocar brasiliense Camb.) Attenuates the Adverse Effects of Cyclical Heat Stress and Modulates the Oxidative Stress-Related Genes in Broiler Chickens

by Jéssica Moraes Cruvinel, Priscila Michelin Groff Urayama, Cássio Yutto Oura, Fernanda Kaiser de Lima Krenchinski, Tatiane Souza dos Santos, Beatriz Alves de Souza, Samir Moura Kadri, Camila Renata Correa, José Roberto Sartori and Antonio Celso Pezzato

Animals 2023, 13(12), 1896; https://doi.org/10.3390/ani13121896 - 06 Jun 2023

Cited by 2 | Viewed by 1125

Abstract

The present study was conducted to determine the possible antioxidant protection of pequi oil (PO) against cyclic heat stress in broiler chickens and to highlight the application of PO as a promising additive in broiler feed. A total of 400 one-day-old male broiler [...] Read more.

The present study was conducted to determine the possible antioxidant protection of pequi oil (PO) against cyclic heat stress in broiler chickens and to highlight the application of PO as a promising additive in broiler feed. A total of 400 one-day-old male broiler chicks (Cobb 500) were randomly assigned to 2 × 5 factorially arranged treatments: two temperature-controlled rooms (thermoneutral—TN or heat stress—HS for 8 h/day) and five dietary PO levels (0, 1.5, 3.0, 4.5, or 6.0 g/kg diet) for 42 days. Each treatment consisted of eight replicates of five birds. The results showed that HS increased glucose (p = 0.006), triglycerides (p < 0.001), and HDL (p = 0.042) at 21 days and reduced (p = 0.005) serum total cholesterol at 42 days. The results also showed that HS increased the contents of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). In contrast, PO linearly decreased AST (p = 0.048) and ALT (p = 0.020) at 21 and 42 days, respectively. The heterophil-to-lymphocyte ratio in the birds under HS was higher than in those in the TN environment (p = 0.046). Heat stress decreased (p = 0.032) the relative weight of their livers at 21 days. The superoxide dismutase activity increased (p = 0.010) in the HS treatments in comparison to the TN treatments, while the glutathione peroxidase activity in the liver decreased (p < 0.001) at 42 days; however, the activity of catalase had no significant effects. Meanwhile, increasing the dietary PO levels linearly decreased plasma malondialdehyde (p < 0.001) in the birds in the HS environment. In addition, PO reduced (p = 0.027) the expression of Hsp 70 in the liver by 92% when compared to the TN treatment without PO, mainly at the 6.0 g/kg diet level. The expression of Nrf2 was upregulated by 37% (p = 0.049) in response to PO with the 6.0 g/kg diet compared to the HS treatment without PO. In conclusion, PO supplementation alleviated the adverse effects of HS on broilers due to its antioxidant action and modulation of the genes related to oxidative stress, providing insights into its application as a potential feed additive in broiler production. Full article

(This article belongs to the Special Issue Antioxidants, Nutraceuticals, and Functional Food in Animals)

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10 pages, 264 KiB

Open AccessArticle

Dietary Supplementation of Lactobacillus johnsonii RS-7 Improved Antioxidant and Immune Function of Weaned Piglets

by Kai Zhao, Heng Yin, Honglin Yan, Wenjie Tang, Hui Diao, Qi Wang, Renli Qi and Jingbo Liu

Animals 2023, 13(10), 1595; https://doi.org/10.3390/ani13101595 - 10 May 2023

Cited by 4 | Viewed by 1226

Abstract

We investigated the effects of dietary supplementation of lactic acid bacteria on the immune and antioxidant performance of weaned pigs. A total of 128 Duroc × Landrace × Yorkshire piglets weaned on day 28 with an average body weight of 8.95 ± 1.15 [...] Read more.

We investigated the effects of dietary supplementation of lactic acid bacteria on the immune and antioxidant performance of weaned pigs. A total of 128 Duroc × Landrace × Yorkshire piglets weaned on day 28 with an average body weight of 8.95 ± 1.15 kg were selected and randomly divided into four treatment groups according to body weight and sex for a 28-day study. The four dietary treatments were basal diet (CON), and CON with 0.05% (LJ0.05), 0.1% (LJ0.1), and 0.2% (LJ0.2) Lactobacillus johnsonii RS-7, respectively. The lowest feed-to-gain ratio (F:G) was found when LJ0.1 was added to the diet. The addition of compound lactic acid bacteria to the diet increased the concentrations of TP, ALB, IgA, and IgM on day 14 and IgG, IgA, and IgM on day 28 (p < 0.05) in the blood, with trait values greater for pigs fed LJ0.1 than CON pigs (p < 0.05). Concentrations of antioxidants (CAT, T-AOC, MDA, T-SOD, and GSH) in serum, intestinal mucosa, spleen, liver, and pancreas improved. In summary, dietary supplementation of Lactobacillus johnsonii RS-7 improved the antioxidant and immune function of weaned piglets. Full article

(This article belongs to the Special Issue Antioxidants, Nutraceuticals, and Functional Food in Animals)

13 pages, 6663 KiB

Open AccessArticle

Extracts of Apricot (Prunus armeniaca) and Peach (Prunus pérsica) Kernels as Feed Additives: Nutrient Digestibility, Growth Performance, and Immunological Status of Growing Rabbits

by Mohamed Basyony, Amr S. Morsy and Yosra A. Soltan

Animals 2023, 13(5), 868; https://doi.org/10.3390/ani13050868 - 27 Feb 2023

Cited by 1 | Viewed by 1783

Abstract

This study assessed the effects of the kernel extracts of apricot (AKE; Prunus armeniaca) and peach (PKE; Prunus pérsica), and their mixture (Mix) on growth efficiency, feed utilization, cecum activity, and health status, of growing rabbits. Weaned male New Zealand White [...] Read more.

This study assessed the effects of the kernel extracts of apricot (AKE; Prunus armeniaca) and peach (PKE; Prunus pérsica), and their mixture (Mix) on growth efficiency, feed utilization, cecum activity, and health status, of growing rabbits. Weaned male New Zealand White rabbits at six weeks old [n = 84, 736 ± 24 SE g body weight (BW)] were randomly allotted to four dietary groups. The first group received no feed additives (control), the second and third groups received 0.3 mL/kg BW of AKE and PKE, respectively, and the fourth group received a mixture of AKE and PKE (1:1) at 0.3 mL/kg BW (Mix). Results indicated that 2(3h)-Furanone, 5-Heptyldihydro was found in abundance in both extracts, while 1,1-Dimethyl-2 Phenylethy L Butyrate and 1,3-Dioxolane, and 4-Methyl-2-Phenyl- were the most components detected in AKE and Cyclohexanol and 10-Methylundecan-4-olide were found in abundance in PKE. All the experimental extracts enhanced (p < 0.05) the growth performance, cecal fermentation parameters, and cecal L. acidiophilus and L. cellobiosus count, while PKE and the mixture treatments presented the highest (p = 0.001) total weight gain and average weight gain without affecting the feed intake. Rabbits that received the mix treatment had the highest (p < 0.05) nutrient digestibility and nitrogen retained, and the lowest (p = 0.001) cecal ammonia concentration. All the experimental extracts enhanced (p < 0.05) the blood antioxidant indicators (including total antioxidant capacity, catalase, and superoxide dismutase concentrations), and immune response of growing rabbits. In general, fruit kernel extracts are rich sources of bioactive substances that can be used as promising feed additives to promote the growth and health status of weaned rabbits. Full article

(This article belongs to the Special Issue Antioxidants, Nutraceuticals, and Functional Food in Animals)

20 pages, 10530 KiB

Open AccessArticle

Effects of the Vitamin D3 on Alleviating the Oxidative Stress Induced by Diquat in Wenchang Chickens

by Keyi Nong, Youming Liu, Xin Fang, Xinyun Qin, Zhineng Liu and Haiwen Zhang

Animals 2023, 13(4), 711; https://doi.org/10.3390/ani13040711 - 17 Feb 2023

Cited by 7 | Viewed by 1872

Abstract

Vitamin D₃ (VD₃) is an indispensable micronutrient in livestock and poultry feed. Its function in antioxidant stress has been reported. We investigate whether the addition of different concentrations of VD₃ to the diet affects the production performance, slaughter performance, [...] Read more.

Vitamin D₃ (VD₃) is an indispensable micronutrient in livestock and poultry feed. Its function in antioxidant stress has been reported. We investigate whether the addition of different concentrations of VD₃ to the diet affects the production performance, slaughter performance, meat quality, organ index, and gut injury on the diquat (DQ)-induced model of oxidative stress in Wenchang chickens. Four hundred and eighty one-day-old chickens were randomly divided into six groups: control (basal diet), 4000 VD (basal diet + VD₃ 4000 IU per kg feed intake), 1000 VD+DI (DQ, basal diet + VD₃ 1000 IU per kg feed intake), 2000 VD+DI (DQ, basal diet + VD₃ 2000 IU per kg feed intake), and 4000 VD+DI (DQ, basal diet + VD₃ 4000 IU per kg feed intake). The results showed that the addition of VD₃ to the diet promoted DQ-induced weight loss and reduced ADFI, slaughter rate, splenic index, and pH after 1 h and 24 h in the leg muscles. VD₃ decreased the increase in content of interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) among proinflammatory cytokines (p < 0.05) and increased the reduction in anti-inflammatory cytokines content of interleukin-10 (IL-10) (p < 0.05) induced by DQ. In addition, liver and kidney injury biomarkers and the intestinal permeability index in serum were disordered after treatment with DQ (p < 0.05). VD₃ perfected the increase of D-lactic acid (D-LA), diamine oxidase (DAO), total cholesterol (T-CHO), creatinine (CR), blood urea nitrogen (BUN), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C) content, aspartate transaminase (AST), alanine transaminase (ALT), and lactate dehydrogenase (LDH) activity (p < 0.05); it increased the decrease of albumin (ALB) content (p < 0.05). Meanwhile, VD₃ regulated the intestinal morphology and intestinal barrier. Moreover, DQ induced a decrease in total antioxidant capacity and antioxidant enzyme activity in the serum, liver, and jejunum (p < 0.05), and an increase in malonaldehyde (MDA) content (p < 0.05). However, the addition of different levels of VD₃ could alleviate the above phenomenon of oxidative stress in Wenchang chickens to different degrees. Thus, this research suggested that the addition of VD₃ can relieve the DQ-induced oxidative stress of Wenchang chickens, and the level of VD₃ acquisition is positively correlated with the remission effect. Full article

(This article belongs to the Special Issue Antioxidants, Nutraceuticals, and Functional Food in Animals)

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