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Agronomy
Special Issues
Biotechnological Tools Applied to Fruit Trees Breeding
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Biotechnological Tools Applied to Fruit Trees Breeding

A special issue of Agronomy (ISSN 2073-4395). This special issue belongs to the section "Horticultural and Floricultural Crops".

Deadline for manuscript submissions: closed (30 June 2021) | Viewed by 3955

Special Issue Editor

Dr. Nuria Alburquerque Ferrando

E-Mail Website
Guest Editor
CEBAS- CSIC, Centro de Edafología y Biología Aplicada del Segura, Murcia, Spain
Interests: fruit breeding; genetic transformation; in vitro culture; fruit disease resistance; abiotic stress; fruit reproductive biology

Special Issue Information

Dear Colleagues,

Traditionally fruit tree breeding has been approached using classical hybridization techniques. However, the development of new biotechnological techniques allows genetic plant modification in a faster and more precise way. These techniques enable the introduction or modification of specific characters in fruit species while maintaining the general characteristics of a selected cultivar. Plant breeders can incorporate valuable new biotechnology tools that have been developed in recent years like genome-editing techniques, cisgenesis/intragenesis, RNA interference (RNAi), or trans-grafting to obtain desirable fruit cultivars more efficiently that can meet the demand for sustainable agricultural productivity.

This Special Issue is focused on the use of new biotechnological techniques for improving fruit species looking for resistance to biotic or abiotic stresses, modifying traits, etc. Research or review articles about biotechnological techniques limitations or biosafety considerations of the new genetically modified plants obtained with the new technologies are welcome.

Dr. Nuria Alburquerque Ferrando
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Agronomy is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • fruit breeding
  • genome editing
  • RNA interference
  • trans-grafting
  • biotic stress
  • abiotic stress
  • biosafety regulation

Published Papers (2 papers)

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Research

11 pages, 1043 KiB  
Article
Low Outcrossing from an Apple Field Trial Protected with Nets
by Ina Schlathölter, Anna Dalbosco, Michael Meissle, Andrea Knauf, Alex Dallemulle, Beat Keller, Jörg Romeis, Giovanni A. L. Broggini and Andrea Patocchi
Agronomy 2021, 11(9), 1754; https://doi.org/10.3390/agronomy11091754 - 31 Aug 2021
Cited by 6 | Viewed by 1500
Abstract
Regulatory compliance of experimental releases into the environment of not yet approved genetically modified plants often requires implementation of measures to reduce the dispersal of reproductive material. To study the impact of nets on pollen flow in an experimental field site in Switzerland, [...] Read more.
Regulatory compliance of experimental releases into the environment of not yet approved genetically modified plants often requires implementation of measures to reduce the dispersal of reproductive material. To study the impact of nets on pollen flow in an experimental field site in Switzerland, non-GM apple varieties ‘Ladina’ and ’Nicogreen’ were planted inside and outside a netted plot, respectively. Seeds harvested from mature fruits were germinated and the paternal variety of the seedlings was determined using simple sequence repeat (SSR) molecular markers. We demonstrate that pollination frequency from trees inside the netted plot to trees outside over a two-year (2018 and 2019) study of 4500 seedlings is 0.6% (26 seedlings). Moreover, these outcrossing events decreased with increasing distance from the pollen donor. Over the study period, we found on average 0.9%, 0.5%, 0.4% and 0.09% of the seedlings derived from apples of ‘Nicogreen’ trees at 8 m, 15 m, 72 m and 117 m being generated by fertilisations of ‘Ladina’ pollen, respectively. In comparison, 48.3% (2018 season) and 75.1% (2019 season) of examined ‘Ladina’ seedlings in the netted plot originated from ‘Nicogreen’ tree pollen outside the netted plot. The results suggest that insect netting is effective in minimizing egress of apple pollen from an experimental site and that the likelihood of outcrossing is reduced further (<0.1%) when there are no compatible apple trees within a radius of 100 m of the pollen donor. These data are important for biosafety research/regulation to aid understanding of pollen flow in experimental field sites. Full article
(This article belongs to the Special Issue Biotechnological Tools Applied to Fruit Trees Breeding)
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10 pages, 1847 KiB  
Article
Improving Adventitious Shoot Regeneration and Transient Agrobacterium-Mediated Transformation of Apricot (Prunus armeniaca L.) Hypocotyl Sections
by Cristian Pérez-Caselles, Lydia Faize, Lorenzo Burgos and Nuria Alburquerque
Agronomy 2021, 11(7), 1338; https://doi.org/10.3390/agronomy11071338 - 30 Jun 2021
Cited by 3 | Viewed by 1955
Abstract
The improvement of previously described protocols for the regeneration of shoots from ‘Canino’ mature seed hypocotyl slices has been accomplished. The effects of different factors such as the part of the hypocotyl used, vacuum-infiltration, 2,4-Dichlorophenoxyacetic acid pulse, vacuum-infiltration and sonication on regeneration and [...] Read more.
The improvement of previously described protocols for the regeneration of shoots from ‘Canino’ mature seed hypocotyl slices has been accomplished. The effects of different factors such as the part of the hypocotyl used, vacuum-infiltration, 2,4-Dichlorophenoxyacetic acid pulse, vacuum-infiltration and sonication on regeneration and transient transformation were analyzed. When the three slices obtained from the hypocotyls were evaluated separately on regeneration medium, the highest percentages of regenerating explants were achieved in the part close to the epicotyl and in the central part. On the other hand, sonication of the explants for 30 s followed by vacuum-infiltration during Agrobacterium infection for 20 min allowed for an increase in the transformation events. The application of these modifications to the procedure increased the regeneration efficiencies, and transient transformation events and may reduce the frequency of failed experiments. An efficient regeneration/transformation protocol could facilitate its use as a biotechnological technique for apricot breeding. Full article
(This article belongs to the Special Issue Biotechnological Tools Applied to Fruit Trees Breeding)
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