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Volume 2
Issue 4
10.3390/micro2040041
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Article
Peer-Review Record

Possibility of Biological Observations Using the Speckle Interferometry-Based Super-Resolution Technology

Micro 2022, 2(4), 620-631; https://doi.org/10.3390/micro2040041
by Yasuhiko Arai
Reviewer 1:
Yingjie Yu
Reviewer 2: Anonymous
Micro 2022, 2(4), 620-631; https://doi.org/10.3390/micro2040041
Submission received: 1 October 2022 / Revised: 19 October 2022 / Accepted: 27 October 2022 / Published: 31 October 2022
(This article belongs to the Collection Advances in Microtechnology for Cell/Tissue Engineering and Biosensing)

Round 1

Reviewer 1 Report

This study examined the possibility of using a new speckle interferometry-based observation technique used in the field of biotechnology. It got some good results. It is suggested to describe more how to get high accuracy of the method whole improve the resolution.

 

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

Reviewer 2 Report

The manuscript entitled “Possibility of biological observations using the speckle

 interferometry-based super-resolution technology” is scientifically interesting and original.

 The text is well written, scientifically thorough and with correct language and syntax.

  However, there are some points in the manuscript that need improvement:

  In my opinion, the words in the paper's title should not be repeated in the keywords. So the words: “Super-resolution; Biological observation;” should be deleted or replaced by other words or phrases.

 

The introduction chapter may need to be strengthened by more articles, such as (among others) the following (lines 24-27):

 

  1. Photoactivatable Fluorophore for Stimulated Emission Depletion (STED) Microscopy and Bioconjugation Technique for Hydrophobic Labels, https://doi.org/10.1002/chem.202004645
  2. S. W. Hell, M. Kroug, Appl. Phys. B: Lasers and Opt. 1995, 60, 495– 497
  3. Fluorometric determination of formaldehyde, Fresenius' Journal of Analytical Chemistry, 1997, 358(5), pp. 667–668
  4. PHOTOACTIVABLE FLUOROPHORES .3. SYNTHESIS AND PHOTOACTIVATION OF FLUOROGENIC DIFUNCTIONALIZED FLUORESCEINS, Journal of America Chemical Society, 1988, 110, 1, 31-303.

 Lines:46-48

 

  1. The Effect of Shape on Intermethod Correlation of Techniques for Characterizing the Size Distribution of Powder. Part 2: Correlating the Size Distribution as Measured by Diffractometer Methods, TSI-Amherst Aerosol Spectrometer, and Coulter Counter

2.Principles of optics - electromagnetic theory of propagation, interference and diffraction of light (7. ed.)

 

 

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

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