Glycerol Monolaurate Alleviates Oxidative Stress and Intestinal Flora Imbalance Caused by Salinity Changes for Juvenile Grouper
Abstract
:1. Introduction
2. Materials and Methods
2.1. Diets Preparation
2.2. Experimental Design
2.3. Sample Collection
2.4. Liver Antioxidant Index
2.5. Real-Time Quantitative RT-PCR Analysis of Gene Expression
2.6. Intestinal Flora Analysis
2.6.1. Extraction of Genomic DNA
2.6.2. PCR Amplification of Target Regions
2.6.3. PCR Product Purification, Library Construction and Sequencing
2.6.4. Data Analysis and Functional Prediction
2.7. Statistical Analysis
3. Results
3.1. Effects of GML on Antioxidant Enzyme Activities Caused by Salinity Changes for Grouper
3.2. Effects of GML on Gene Expression Caused by Salinity Changes in Grouper
3.3. Effects of GML on Intestinal Flora Caused by Salinity Changes in Grouper
3.3.1. Effects of GML on Relative Abundance at the Phylum Level Caused by Salinity Changes in Grouper
3.3.2. Effects of GML on Relative Abundance at the Genus Level Caused by Salinity Changes in Grouper
3.3.3. Effects of GML on Indicator Species Caused by Salinity Changes in Grouper
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Ingredients | G0 | G1800 |
---|---|---|
Brown fish meal | 30.00 | 30.00 |
Soybean meal | 18.00 | 18.00 |
Corn gluten meal | 9.50 | 9.50 |
Peanut meal | 10.00 | 10.00 |
Chicken powder | 6.00 | 6.00 |
Wheat flour | 18.59 | 18.59 |
Fish oil | 2.00 | 2.00 |
Soybean oil | 2.00 | 2.00 |
Soybean lecithin | 1.00 | 1.00 |
Ca(H2PO4)2 | 1.00 | 1.00 |
Premix | 1.00 | 1.00 |
Choline chloride | 0.50 | 0.50 |
Vitamin C | 0.05 | 0.05 |
Microcrystalline cellulose | 0.36 | 0.18 |
GML | 0.00 | 0.18 |
Total | 100.00 | 100.00 |
Nutrient levels | ||
Crude protein | 49.74 | 49.66 |
Crude lipid | 9.42 | 9.37 |
Genes | Nucleotide Sequence (5′-3) | Genbank Accession No. |
---|---|---|
β-actin | F: GATCTGGCATCACACCTTCT R: CATCTTCTCCCTGTTGGCTT | AY510710.2 |
SOD | F: GTTGGAGACCTGGGAAATGTGACTG R: CCATTGAGGGTGAGCATCTTGTCC | AY735008.1 |
CAT | F:GCTCTATCCGCTCCTCTTCTCCTC R: GTAGTTCCTGACGACGGTGATGTG | KT884509.1 |
IL6 | F: CAATCCCAGCACCTTCCAC R: CCTGACAGCCAGACTTCCTCT | AFE62919.1 |
Itmes | G0-0 | G0-1800 | G6-0 | G6-1800 | G12-0 | G12-1800 | G24-0 | G24-1800 |
---|---|---|---|---|---|---|---|---|
Proteobacteria | 99.072 | 87.608 | 87.832 | 77.375 | 80.998 | 70.044 | 94.666 | 97.028 |
Firmicutes | 0.794 | 12.205 | 11.683 | 22.513 | 18.841 | 29.836 | 3.524 | 2.908 |
Actinobacteria | 0.050 | 0.133 | 0.004 | 0.003 | 0.011 | 0.009 | 0.015 | 0.011 |
Bacteroidetes | 0.052 | 0.029 | 0.403 | 0.083 | 0.112 | 0.086 | 1.749 | 0.027 |
Cyanobacteria | 0.011 | 0.004 | 0.056 | 0.000 | 0.005 | 0.002 | 0.010 | 0.000 |
Others | 0.020 | 0.019 | 0.007 | 0.008 | 0.000 | 0.000 | 0.001 | 0.000 |
Unclassified | 0.002 | 0.002 | 0.016 | 0.017 | 0.033 | 0.022 | 0.035 | 0.026 |
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Li, X.; Zhu, D.; Mao, M.; Wu, J.; Yang, Q.; Tan, B.; Chi, S. Glycerol Monolaurate Alleviates Oxidative Stress and Intestinal Flora Imbalance Caused by Salinity Changes for Juvenile Grouper. Metabolites 2022, 12, 1268. https://doi.org/10.3390/metabo12121268
Li X, Zhu D, Mao M, Wu J, Yang Q, Tan B, Chi S. Glycerol Monolaurate Alleviates Oxidative Stress and Intestinal Flora Imbalance Caused by Salinity Changes for Juvenile Grouper. Metabolites. 2022; 12(12):1268. https://doi.org/10.3390/metabo12121268
Chicago/Turabian StyleLi, Xuehe, Dongwenjun Zhu, Minling Mao, Jianwei Wu, Qihui Yang, Beiping Tan, and Shuyan Chi. 2022. "Glycerol Monolaurate Alleviates Oxidative Stress and Intestinal Flora Imbalance Caused by Salinity Changes for Juvenile Grouper" Metabolites 12, no. 12: 1268. https://doi.org/10.3390/metabo12121268