Application Marker-Assisted Selection (MAS) and Multiplex PCR Reactions in Resistance Breeding of Maize (Zea mays L.)
, Agnieszka Tomkowiak 1, Jan Bocianowski 2, Bartosz Nowak 3, Dorota Weigt 1, Danuta Kurasiak-Popowska 1, Michał Kwiatek 1, Sylwia Mikołajczyk 1, Janetta Niemann 1 and Katarzyna Szewczyk 1Round 1
Reviewer 1 Report
I want to thank authors about great article.
1-for the methods of DNA extraction you did not mention the references
2- the concentrations of DNA range 155-950 ng but I think the best concentration for PCR is range from 50-100 ng
Author Response
Please see the attachment.
Author Response File: 
Author Response.pdf
Reviewer 2 Report
This research paper has major scientific flaws as these were pointed-out below:
1. Title of research is not according to the research work.
2. Research problem addressed in abstract and the research design not in line.
3. Objective of the study and outcome of the research not in the same direction.
3. Lack of scientific writing e.g, line245: photos instead of figure , article formate e.g., abstract has three paragraphs (it should be wrote in single paragraph) and introduction have too small paragraphs.
4. Methodology is not appropriate.
5. Lack of research novelty. As lines used already known resistant/ susceptible to Fusarium, and markers employed also reported earlier and confirmed the association with Fusarium tolerance.
Author Response
Please see the attachment.
Author Response File: Author Response.pdf
Reviewer 3 Report
1- Please explains resistance for Fusarium spp. or specific fusarium disease like Fusarium wilt etc. (Title as well)
2-conclusion must be clear and precise (in Abstract)
3- Update area, yield and production of crop for 2022, style of references citing in the text is different throughout paper (e.g, reference(2,4, 10 etc.), so uniform it as per journal policy (introduction)
4-As resistant and susceptible genotypes already mentioned are used then what is the role of this study for further . please justify your goals and results and also add recommendations for farming community (Conclusion of paper)
Comments for author File: Comments.pdf
Author Response
Please see the attachment.
Author Response File: Author Response.pdf
Round 2
Reviewer 2 Report
Author tried to improve the research article in much better way. But still there is few improvements required to improve the scientific soundness and significance of content.
1: In introduction author used quite small paragraphs, thats showed each paragraph have different context and decrease the significance of content. as strategies to tackle the disease write in one paragraph, do not split into two paragraph. Similarly, in past success stories of MAS selection and application in breeding need to write in one paragraph.
2: Write about the importance, significance of multiplex PCR in breeding process in introduction section.
3: Please provide the pedigree information of lines used in this study.
4: remove the heading "3.2 DNA isolation" unnecessary to describe in results.
5: Use the word figure instead of photo
6: Is marker used in this study single or multi-featured markers? please explained markers feature.
Author Response
Author tried to improve the research article in much better way. But still there is few improvements required to improve the scientific soundness and significance of content.
Dear reviewer thank you very much for this review.
1: In introduction author used quite small paragraphs, thats showed each paragraph have different context and decrease the significance of content. as strategies to tackle the disease write in one paragraph, do not split into two paragraph. Similarly, in past success stories of MAS selection and application in breeding need to write in one paragraph.
In accordance with the comments of the honorable Reviewer,the introduction has been corrected.
2: Write about the importance, significance of multiplex PCR in breeding process in introduction section.
In accordance with the comments of the honorable Reviewer,information about significant multiplex PCR hen been added.
3: Please provide the pedigree information of lines used in this study.
Dear reviewer, the inbred lines used for the research were derived from hybrid varieties available on the Polish market. They are mainly characterized by grain types of Dent. Hybrids from which the inbred lines were derived belong to the BSSS and non-BSSS origin groups, mainly Iodent and Lancaster. This information was added to the publication
4: remove the heading "3.2 DNA isolation" unnecessary to describe in results.
In accordance with the comments of the honorable Reviewer, this heading has been removed.
5: Use the word figure instead of photo
In accordance with the comments of the honorable Reviewer, the summary was re-written. The photo have been turned into figure.
6: Is marker used in this study single or multi-featured markers? please explained markers feature.
The trait is polygenic, while the marker is to identify the locus of this gene. We use the SSR and ISSR markers
