Hybrid Nanoparticle-Assisted Chemo-Photothermal Therapy and Photoacoustic Imaging in a Three-Dimensional Breast Cancer Cell Model
Abstract
:1. Introduction
2. Results and Discussion
2.1. NPs Characterisation
2.2. Evaluation of Bare- and HSA-NP Uptake in Hs578T and MCF10a Spheroids
2.3. DOX Delivery in Hs578T Spheroids
2.4. Light Microscopy Analysis of Hs578T Spheroids after DOX@NPs Treatment
2.5. Cytotoxicity of DOX@NPs
2.6. Cytotoxicity of DOX@NPs Combined with Laser Irradiation
2.7. Photoacoustic Imaging of Hs578T Spheroids
3. Materials and Methods
3.1. Materials
3.2. Nanoparticles Preparation and Characterisation
3.3. Cell Culture
3.4. Spheroid Formation
3.5. Confocal and Thunder Microscopy
3.6. Light Microscopy Analysis
3.7. Cell Viability Assay
3.8. High-Frequency Ultrasound and Photoacoustic Imaging of Hs578T Spheroids
3.9. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Size (nm) | PDI | ζ-Potential (mV) | |
---|---|---|---|
MelaSil_Ag | 353 ± 17 | 0.33 ± 0.1 | −22 ± 1.3 |
MelaSil_Ag-HSA | 384 ± 30 | 0.26 ± 0.07 | −27.2 ± 1.8 |
MelaSil_Ag-HSA@DOX | 407 ± 29 | 0.45 ± 0.09 | −17 ± 2.16 |
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Carrese, B.; Cavallini, C.; Armanetti, P.; Silvestri, B.; Calì, G.; Luciani, G.; Sanità, G.; Menichetti, L.; Lamberti, A. Hybrid Nanoparticle-Assisted Chemo-Photothermal Therapy and Photoacoustic Imaging in a Three-Dimensional Breast Cancer Cell Model. Int. J. Mol. Sci. 2023, 24, 17374. https://doi.org/10.3390/ijms242417374
Carrese B, Cavallini C, Armanetti P, Silvestri B, Calì G, Luciani G, Sanità G, Menichetti L, Lamberti A. Hybrid Nanoparticle-Assisted Chemo-Photothermal Therapy and Photoacoustic Imaging in a Three-Dimensional Breast Cancer Cell Model. International Journal of Molecular Sciences. 2023; 24(24):17374. https://doi.org/10.3390/ijms242417374
Chicago/Turabian StyleCarrese, Barbara, Chiara Cavallini, Paolo Armanetti, Brigida Silvestri, Gaetano Calì, Giuseppina Luciani, Gennaro Sanità, Luca Menichetti, and Annalisa Lamberti. 2023. "Hybrid Nanoparticle-Assisted Chemo-Photothermal Therapy and Photoacoustic Imaging in a Three-Dimensional Breast Cancer Cell Model" International Journal of Molecular Sciences 24, no. 24: 17374. https://doi.org/10.3390/ijms242417374