Topic Editors

Department of Forensic Toxicology, Institute of Forensic Sciences, Ankara University, Ankara, Turkey
Prof. Dr. Emirhan Nemutlu
Department of Analytical Chemistry, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey
Department of Analytical Chemistry, Faculty of Pharmacy, Ankara University, Ankara, Turkey

Pharmaceutical and Biomedical Applications in Separation Techniques: Recent Trends and Developments

Abstract submission deadline
closed (31 January 2024)
Manuscript submission deadline
31 March 2024
Viewed by
4351

Topic Information

Dear Colleagues,

The analysis of pharmaceuticals, drugs and their metabolites, and biomolecules (proteins, peptides, oligonucleotides, etc.) from different matrices (plasma, serum, oral fluid, urine, etc.) is of great interest to academia and industry. The development of new and green extraction methodologies and validated analytical methods of separation and analysis is therefore of the utmost importance.

Chromatographic techniques such as liquid and gas chromatography are the most frequently used analytical techniques. In addition to these, miniaturized techniques such as capillary electrophoresis, nano-liquid chromatography, and lab-on-a-chip devices are also of great interest; hyphenated detection techniques improve the state of the art of conventional analytical instrumentation. Furthermore, with the further evolution of the classical techniques, instrumental methodologies such as ultra-performance liquid chromatography, supercritical fluid chromatography, capillary electrochromatography, and 2D and 3D systems have seen significant enhancement.

This topic aims to put together original research papers and review articles that demonstrate the recent advances in separation techniques, including all aspects of extraction methodologies, method development, analytical method validation, and application to the various matrices. We therefore encourage authors to submit analyses of chiral and achiral pharmaceuticals, drugs, impurities from pharmaceutical dosage forms, newly developed drug delivery systems, biological media (therapeutic drug monitoring, etc.), environmental samples, targeted and untargeted omics (metabolomics, proteomics, lipidomics, etc.), and substances of abuse.

Dr. Mehmet Gumustas
Prof. Dr. Emirhan Nemutlu
Prof. Dr. Sibel A. Ozkan
Topic Editors

Keywords

  • sample preparation
  • liquid chromatography
  • gas chromatography
  • separation techniques
  • mass spectrometry
  • chiral molecules
  • biological samples
  • pharmaceuticals
  • natural products
  • omics
  • drug delivery systems
  • censors

Participating Journals

Journal Name Impact Factor CiteScore Launched Year First Decision (median) APC
Metabolites
metabolites
4.1 5.3 2011 13.2 Days CHF 2700 Submit
Molecules
molecules
4.6 6.7 1996 14.6 Days CHF 2700 Submit
Pharmaceuticals
pharmaceuticals
4.6 4.7 2004 14.6 Days CHF 2900 Submit
Pharmaceutics
pharmaceutics
5.4 6.9 2009 14.2 Days CHF 2900 Submit
Separations
separations
2.6 2.5 2014 13.6 Days CHF 2600 Submit

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Published Papers (4 papers)

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18 pages, 4412 KiB  
Article
In Vitro Wound Healing Potential of a Fibroin Film Incorporating a Cannabidiol/2-Hydroxypropyl-β-cyclodextrin Complex
Pharmaceutics 2023, 15(12), 2682; https://doi.org/10.3390/pharmaceutics15122682 - 27 Nov 2023
Cited by 1 | Viewed by 990
Abstract
This study aimed to develop a film dressing prepared by incorporating a complex of cannabidiol and 2-hydroxypropyl-β-cyclodextrin (CBD/HP-β-CD) into a fibroin-based film and to investigate its wound healing capabilities. The fibroin from silkworm cocoons exhibited a total protein content of 96.34 ± 0.14% [...] Read more.
This study aimed to develop a film dressing prepared by incorporating a complex of cannabidiol and 2-hydroxypropyl-β-cyclodextrin (CBD/HP-β-CD) into a fibroin-based film and to investigate its wound healing capabilities. The fibroin from silkworm cocoons exhibited a total protein content of 96.34 ± 0.14% w/w and a molecular weight range of 25 to 245 kDa. Fourier-transform infrared spectroscopy (FTIR) revealed the presence of characteristic amide peaks (I, II, and III) in the isolated fibroin. The CBD/HP-β-CD complex, prepared with a molar ratio of 1:2 (CBD to HP-β-CD), had 81.5 ± 1.2% w/w CBD content, as determined by high-performance liquid chromatography (HPLC). X-ray diffraction (XRD) and FTIR analyses demonstrated successful encapsulation of CBD’s hydrophobic aromatic rings by HP-β-CD. Blending the fibroin solution with the CBD/HP-β-CD complex produced a transparent, slightly yellowish film. Mechanical testing revealed a tensile strength of 48.67 ± 2.57 MPa and a % elongation at a break of 1.71 ± 0.21%. XRD and FTIR analyses showed distinctive crystalline and chemical structures of the film. In subsequent in vitro experiments with normal human dermal fibroblasts, the film demonstrated potential for wound healing. An increase in cell division (G2/M phase) was observed compared to the fibroin film without the CBD/HP-β-CD complex. Additionally, fibroblasts treated with the film exhibited enhanced cell migration in a scratch assay and increased expression of vascular endothelial growth factor protein compared to the control group. Overall, these findings underscore the film’s potential for enhancing wound healing outcomes. Full article
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13 pages, 1689 KiB  
Article
OMNI: Gas Chromatograph Captures Seven Common PET Radiotracer Analytes in under 5 Minutes
Pharmaceuticals 2023, 16(11), 1623; https://doi.org/10.3390/ph16111623 - 17 Nov 2023
Viewed by 683
Abstract
A novel gas chromatography method was developed using automatic injections to identify and quantify the amount of residual solvents or analytes in samples of fluorine-18 and carbon-11 radiopharmaceuticals. This approach evaluates seven analytes in less than 5 versus 13 min of acquisition time. [...] Read more.
A novel gas chromatography method was developed using automatic injections to identify and quantify the amount of residual solvents or analytes in samples of fluorine-18 and carbon-11 radiopharmaceuticals. This approach evaluates seven analytes in less than 5 versus 13 min of acquisition time. The method additionally includes a 3 min bakeout to aid in the removal and carry-over of higher-boiling impurities. Chromatographic parameters such as column temperature, hold time, column pressure, flow rate, and split ratios were adjusted and optimized to analyze radioactive drug samples containing analytes which include methanol, ethanol, acetone, acetonitrile, triethylamine, N,N-dimethylformamide, and dimethyl sulfoxide. The relative standard deviation for each solvent was determined to be no greater than 1.6%. The method limit of detection (LOD) and limit of quantification (LOQ) were between 0.053 and 0.163 and 0.000 (5.791 × 10−6) and 0.520 mg/mL, respectively. This GC technique, using flame ionization detection (FID), was validated and is currently employed for the routine quality control of all approved IND and RDRC PET radiopharmaceuticals at our center. Full article
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15 pages, 3776 KiB  
Article
Interface-Based Design of High-Affinity Affibody Ligands for the Purification of RBD from Spike Proteins
Molecules 2023, 28(17), 6358; https://doi.org/10.3390/molecules28176358 - 30 Aug 2023
Viewed by 851
Abstract
The outbreak of coronavirus disease 2019 (COVID-19) has sparked an urgent demand for advanced diagnosis and vaccination worldwide. The discovery of high-affinity ligands is of great significance for vaccine and diagnostic reagent manufacturing. Targeting the receptor binding domain (RBD) from the spike protein [...] Read more.
The outbreak of coronavirus disease 2019 (COVID-19) has sparked an urgent demand for advanced diagnosis and vaccination worldwide. The discovery of high-affinity ligands is of great significance for vaccine and diagnostic reagent manufacturing. Targeting the receptor binding domain (RBD) from the spike protein of severe acute respiratory syndrome-coronavirus 2, an interface at the outer surface of helices on the Z domain from protein A was introduced to construct a virtual library for the screening of ZRBD affibody ligands. Molecular docking was performed using HADDOCK software, and three potential ZRBD affibodies, ZRBD-02, ZRBD-04, and ZRBD-07, were obtained. Molecular dynamics (MD) simulation verified that the binding of ZRBD affibodies to RBD was driven by electrostatic interactions. Per-residue free energy decomposition analysis further substantiated that four residues with negative-charge characteristics on helix α1 of the Z domain participated in this process. Binding affinity analysis by microscale thermophoresis showed that ZRBD affibodies had high affinity for RBD binding, and the lowest dissociation constant was 36.3 nmol/L for ZRBD-07 among the three potential ZRBD affibodies. Herein, ZRBD-02 and ZRBD-07 affibodies were selected for chromatographic verifications after being coupled to thiol-activated Sepharose 6 Fast Flow (SepFF) gel. Chromatographic experiments showed that RBD could bind on both ZRBD SepFF gels and was eluted by 0.1 mol/L NaOH. Moreover, the ZRBD-07 SepFF gel had a higher affinity for RBD. This research provided a new idea for the design of affibody ligands and validated the potential of affibody ligands in the application of RBD purification from complex feedstock. Full article
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12 pages, 279 KiB  
Review
Measurement of Steroids in the Placenta, Maternal Serum, and Fetal Serum in Humans, Rats, and Mice: A Technical Note
Separations 2023, 10(4), 221; https://doi.org/10.3390/separations10040221 - 23 Mar 2023
Viewed by 1066
Abstract
Steroid hormones are vital for a successful pregnancy. The placenta is attached to the uterine wall and is the major organ of communication between the mother and the fetus through the umbilical cord and the transfer of compounds (including the production and actions [...] Read more.
Steroid hormones are vital for a successful pregnancy. The placenta is attached to the uterine wall and is the major organ of communication between the mother and the fetus through the umbilical cord and the transfer of compounds (including the production and actions of steroids) across the villous placenta. Therefore, a correct understanding and measurement of steroid levels across the maternal–placental–fetal interface is essential. We have experience spanning more than two decades and have published more than 40 papers using a variety of methods to assess circulating and placental steroid levels. In this review, we discuss various methods for steroid detection and quantitation, as well as their advantages and disadvantages. This document provides technical guidance for best practices that, in our estimation, can assist researchers in more easily and correctly performing these studies. Critical methodological considerations, including tissue collection, tissue processing, and analytical factors (sensitivity, selectivity, matrix effects, and internal standards), are covered. We highlight important differences between human and rodent tissues as they relate to steroid levels in pregnancy and the interpretation of results, and provide guidance for best practices in future studies. Full article
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