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3 pages, 162 KiB

Open AccessEditorial

Surface Plasmon Resonance (SPR)-Based Biosensors as Instruments with High Versatility and Sensitivity

by Victoria Shpacovitch and Roland Hergenröder

Sensors 2020, 20(11), 3010; https://doi.org/10.3390/s20113010 - 26 May 2020

Cited by 22 | Viewed by 2919

Abstract

Surface plasmon resonance (SPR), as a physical phenomenon, is not restricted only to events occurring in thin planar metal films [...] Full article

(This article belongs to the Special Issue Surface Plasmon Resonance (SPR)-Based Sensors and Their Biological Applications)

Research

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9 pages, 2002 KiB

Open AccessArticle

Potential Application of Gold Nanospheres as a Surface Plasmon Resonance Based Sensor for In-Situ Detection of Residual Fungicides

by Hang Nguyen Thi Nhat, Ngoc Thuy Trang Le, Nguyen Thi Phuong Phong, Dai Hai Nguyen and Minh-Tri Nguyen-Le

Sensors 2020, 20(8), 2229; https://doi.org/10.3390/s20082229 - 15 Apr 2020

Cited by 6 | Viewed by 2932

Abstract

It is essential to develop a simple and sensitive method to rapidly detect residual fungicides in agricultural products to protect human health. So far, little studies have been reported on potential application of gold nanospheres (AuNSps) as a surface plasmon resonance based sensor [...] Read more.

It is essential to develop a simple and sensitive method to rapidly detect residual fungicides in agricultural products to protect human health. So far, little studies have been reported on potential application of gold nanospheres (AuNSps) as a surface plasmon resonance based sensor for in-situ detection of residual fungicides. Therefore, in this study, we investigated the potential application of AuNSps as a surface plasmon resonance based sensor for in-situ detection of fungicides. AuNSps were successfully synthesized via a seed-mediated method with some modifications. Firstly, gold nanoseeds were made during the reduction of chloroauric acid by trisodium citrate dihydrate (TSC). Then, AuNSps were grown from the seeds by using HAuCl₄, TSC and EDTA. AuNSps were subsequently dropped on a glass substrate before covered by thiophanate methyl, a broad-spectrum systemic fungicide. The AuNSps coated glass substrate was subsequently dried in the air for further surface-enhanced Raman spectroscopy (SERS) measurements. Optical properties, shape and size of AuNSps were confirmed by UV-vis spectroscopy, XRD, SEM-EDX and TEM. The results showed that AuNSps were successfully synthesized with the size of 53 nm, and their resonance peak was located at 560 nm. The Raman signal intensity of thiophanate methyl covered on AuNSps is higher than that without AuNSps, indicating SERS effects of AuNSps deposited glass substrate. Full article

(This article belongs to the Special Issue Surface Plasmon Resonance (SPR)-Based Sensors and Their Biological Applications)

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12 pages, 3307 KiB

Open AccessArticle

High Sensitive Biosensors Based on the Coupling Between Surface Plasmon Polaritons on Titanium Nitride and a Planar Waveguide Mode

by Junior Asencios, Ramiro Moro, Clemente Luyo and Arturo Talledo

Sensors 2020, 20(6), 1784; https://doi.org/10.3390/s20061784 - 23 Mar 2020

Cited by 10 | Viewed by 3287

Abstract

High sensitivity biosensors based on the coupling of surface plasmon polaritons on titanium nitride (TiN) and a planar waveguide mode were built; they were proved by sensing three different media: air, water and dried egg white; sensors described here could be useful for [...] Read more.

High sensitivity biosensors based on the coupling of surface plasmon polaritons on titanium nitride (TiN) and a planar waveguide mode were built; they were proved by sensing three different media: air, water and dried egg white; sensors described here could be useful for sensing materials with a refractive index between 1.0 and 1.6; in particular, materials of biological interest with a refractive index in the range 1.3–1.6, like those containing biotin and/or streptavidin. They were built by depositing Nb₂O₅/SiO₂/TiN multilayer structures on the flat surface of D-shaped sapphire prisms by using the dc magnetron sputtering technique. Attenuated total reflection (ATR) experiments in the Kretschmann configuration were accomplished for the air/TiN/Prism and S/Nb₂O₅/SiO₂/TiN/Prism structures, S being the sample or sensing medium. ATR spectra for plasmons at the TiN/air interface showed a broad absorption band for angles of incidence between 36 and 85°, with full width at half maximum (FWHM) of approximately 40°. For the S/Nb₂O₅/SiO₂/TiN/Prism structures, ATR spectra showed a sharp reflectivity peak, within the broad plasmonic absorption band, which was associated with Fano resonances. The angular position and FWHM of the Fano resonances strongly depend on the refractive index of the sensing medium. ATR spectra were fitted by using the transfer-matrix method. Additionally, we found that angular sensitivity and figure of merit increase with increasing the refractive index of the sensing medium. Full article

(This article belongs to the Special Issue Surface Plasmon Resonance (SPR)-Based Sensors and Their Biological Applications)

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12 pages, 4536 KiB

Open AccessArticle

Sensitivity Enhancement of a Concave Shaped Optical Fiber Refractive Index Sensor Covered with Multiple Au Nanowires

by A. K. Pathak, B. M. A. Rahman, V. K. Singh and S. Kumari

Sensors 2019, 19(19), 4210; https://doi.org/10.3390/s19194210 - 27 Sep 2019

Cited by 50 | Viewed by 4806

Abstract

In the present paper, a new kind of concave shaped refractive index sensor (CSRIS) exploiting localized surface plasmon resonance (LSPR) is proposed and numerically optimized. The LSPR effect between polaritons and the core guided mode of designed CSRIS is used to enhance the [...] Read more.

In the present paper, a new kind of concave shaped refractive index sensor (CSRIS) exploiting localized surface plasmon resonance (LSPR) is proposed and numerically optimized. The LSPR effect between polaritons and the core guided mode of designed CSRIS is used to enhance the sensing performance. The sensor is characterized for two types of sensing structures coated with gold (Au) film and Au nanowires (AuNWs), respectively. The influence of structural parameters such as the distance (D) of the concave shaped channel (CSC) from the core, the diameter of the nanowire (d_n) and the size (s) of the CSC are investigated here. In comparison to Au film, the AuNWs are shown to significantly enhance the sensitivity and the performance of the designed sensor. An enhanced sensitivity of 4471 nm/RIU (refractive index unit) is obtained with AuNWs, for a wide range of analytes refractive index (n_a) varying between 1.33 to 1.38. However, for conventional Au film; the sensitivity of 808.57 nm/RIU is obtained for the same range of analytes. Full article

(This article belongs to the Special Issue Surface Plasmon Resonance (SPR)-Based Sensors and Their Biological Applications)

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15 pages, 917 KiB

Open AccessArticle

Nanoparticle Classification Using Frequency Domain Analysis on Resource-Limited Platforms

by Mikail Yayla, Anas Toma, Kuan-Hsun Chen, Jan Eric Lenssen, Victoria Shpacovitch, Roland Hergenröder, Frank Weichert and Jian-Jia Chen

Sensors 2019, 19(19), 4138; https://doi.org/10.3390/s19194138 - 24 Sep 2019

Cited by 9 | Viewed by 3616

Abstract

A mobile system that can detect viruses in real time is urgently needed, due to the combination of virus emergence and evolution with increasing global travel and transport. A biosensor called PAMONO (for Plasmon Assisted Microscopy of Nano-sized Objects) represents a viable technology [...] Read more.

A mobile system that can detect viruses in real time is urgently needed, due to the combination of virus emergence and evolution with increasing global travel and transport. A biosensor called PAMONO (for Plasmon Assisted Microscopy of Nano-sized Objects) represents a viable technology for mobile real-time detection of viruses and virus-like particles. It could be used for fast and reliable diagnoses in hospitals, airports, the open air, or other settings. For analysis of the images provided by the sensor, state-of-the-art methods based on convolutional neural networks (CNNs) can achieve high accuracy. However, such computationally intensive methods may not be suitable on most mobile systems. In this work, we propose nanoparticle classification approaches based on frequency domain analysis, which are less resource-intensive. We observe that on average the classification takes 29

μ

s per image for the Fourier features and 17

μ

s for the Haar wavelet features. Although the CNN-based method scores 1–2.5 percentage points higher in classification accuracy, it takes 3370

μ

s per image on the same platform. With these results, we identify and explore the trade-off between resource efficiency and classification performance for nanoparticle classification of images provided by the PAMONO sensor. Full article

(This article belongs to the Special Issue Surface Plasmon Resonance (SPR)-Based Sensors and Their Biological Applications)

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12 pages, 3205 KiB

Open AccessArticle

Development of SPR Imaging-Impedance Sensor for Multi-Parametric Living Cell Analysis

by Yuhki Yanase, Kyohei Yoshizaki, Kaiken Kimura, Tomoko Kawaguchi, Michihiro Hide and Shigeyasu Uno

Sensors 2019, 19(9), 2067; https://doi.org/10.3390/s19092067 - 03 May 2019

Cited by 16 | Viewed by 4457

Abstract

Label-free evaluation and monitoring of living cell conditions or functions by means of chemical and/or physical sensors in a real-time manner are increasingly desired in the field of basic research of cells and clinical diagnosis. In order to perform multi-parametric analysis of living [...] Read more.

Label-free evaluation and monitoring of living cell conditions or functions by means of chemical and/or physical sensors in a real-time manner are increasingly desired in the field of basic research of cells and clinical diagnosis. In order to perform multi-parametric analysis of living cells on a chip, we here developed a surface plasmon resonance (SPR) imaging (SPRI)-impedance sensor that can detect both refractive index (RI) and impedance changes on a sensor chip with comb-shaped electrodes. We then investigated the potential of the sensor for label-free and real-time analysis of living cell reactions in response to stimuli. We cultured rat basophilic leukemia (RBL)-2H3 cells on the sensor chip, which was a glass slide coated with comb-shaped electrodes, and detected activation of RBL-2H3 cells, such as degranulation and morphological changes, in response to a dinitro-phenol-conjugated human serum albumin (DNP-HSA) antigen. Moreover, impedance analysis revealed that the changes of impedance derived from RBL-2H3 cell activation appeared in the range of 1 kHz–1 MHz. Furthermore, we monitored living cell-derived RI and impedance changes simultaneously on a sensor chip using the SPRI-impedance sensor. Thus, we developed a new technique to monitor both impedance and RI derived from living cells by using a comb-shaped electrode sensor chip. This technique may enable us to clarify complex living cell functions which affect the RI and impedance and apply this to medical applications, such as accurate clinical diagnosis of type I allergy. Full article

(This article belongs to the Special Issue Surface Plasmon Resonance (SPR)-Based Sensors and Their Biological Applications)

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8 pages, 3275 KiB

Open AccessArticle

Sensitivity and Stability Enhancement of Surface Plasmon Resonance Biosensors based on a Large-Area Ag/MoS₂ Substrate

by Nak-Hyeon Kim, Munsik Choi, Tae Woo Kim, Woong Choi, Sang Yoon Park and Kyung Min Byun

Sensors 2019, 19(8), 1894; https://doi.org/10.3390/s19081894 - 21 Apr 2019

Cited by 34 | Viewed by 3818

Abstract

Surface plasmon resonance (SPR) sensors based on a silver film suffer from signal degradation due to silver oxidation in aqueous sensing environments. To overcome this limitation, we fabricated the planar plasmonic substrate employing an atomic MoS₂ layer on a silver surface. Successful [...] Read more.

Surface plasmon resonance (SPR) sensors based on a silver film suffer from signal degradation due to silver oxidation in aqueous sensing environments. To overcome this limitation, we fabricated the planar plasmonic substrate employing an atomic MoS₂ layer on a silver surface. Successful production of a large-area MoS₂ monolayer blocks the penetration of oxygen and water molecules. In addition, we theoretically and experimentally found that MoS₂ layer on the silver film can improve the SPR sensitivity and stability significantly. In this study, the proposed SPR substrate has the potential to provide highly enhanced sensor platforms for surface-limited molecular detections. Full article

(This article belongs to the Special Issue Surface Plasmon Resonance (SPR)-Based Sensors and Their Biological Applications)

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12 pages, 2380 KiB

Open AccessArticle

Early Detection of the Fungal Banana Black Sigatoka Pathogen Pseudocercospora fijiensis by an SPR Immunosensor Method

by Donato Luna-Moreno, Araceli Sánchez-Álvarez, Ignacio Islas-Flores, Blondy Canto-Canche, Mildred Carrillo-Pech, Juan Francisco Villarreal-Chiu and Melissa Rodríguez-Delgado

Sensors 2019, 19(3), 465; https://doi.org/10.3390/s19030465 - 23 Jan 2019

Cited by 41 | Viewed by 4971

Abstract

Black Sigatoka is a disease that occurs in banana plantations worldwide. This disease is caused by the hemibiotrophic fungus Pseudocercospora fijiensis, whose infection results in a significant reduction in both product quality and yield. Therefore, detection and identification in the early stages [...] Read more.

Black Sigatoka is a disease that occurs in banana plantations worldwide. This disease is caused by the hemibiotrophic fungus Pseudocercospora fijiensis, whose infection results in a significant reduction in both product quality and yield. Therefore, detection and identification in the early stages of this pathogen in plants could help minimize losses, as well as prevent the spread of the disease to neighboring cultures. To achieve this, a highly sensitive SPR immunosensor was developed to detect P. fijiensis in real samples of leaf extracts in early stages of the disease. A polyclonal antibody (anti-HF1), produced against HF1 (cell wall protein of P. fijiensis) was covalently immobilized on a gold-coated chip via a mixed self-assembled monolayer (SAM) of alkanethiols using the EDC/NHS method. The analytical parameters of the biosensor were established, obtaining a limit of detection of 11.7 µg mL⁻¹, a sensitivity of 0.0021 units of reflectance per ng mL⁻¹ and a linear response range for the antigen from 39.1 to 122 µg mL⁻¹. No matrix effects were observed during the measurements of real leaf banana extracts by the immunosensor. To the best of our knowledge, this is the first research into the development of an SPR biosensor for the detection of P. fijiensis, which demonstrates its potential as an alternative analytical tool for in-field monitoring of black Sigatoka disease. Full article

(This article belongs to the Special Issue Surface Plasmon Resonance (SPR)-Based Sensors and Their Biological Applications)

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