Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
4.7
3.5
Journals
Processes
Special Issues
Applied Enzymology & Environmental Biotechnology
share announcement

Applied Enzymology & Environmental Biotechnology

A special issue of Processes (ISSN 2227-9717). This special issue belongs to the section "Environmental and Green Processes".

Deadline for manuscript submissions: closed (30 March 2022) | Viewed by 14890

Special Issue Editor

Dr. Habib Horchani

E-Mail Website
Guest Editor
Département des sciences, Groupe de recherche en environnement et biotechnologie, College of Rivière-Du-Loup, Canada
Interests: enzymes; biotransformation; industrial biotechnology; waste management; environmental

Special Issue Information

Dear Colleagues,

To reduce greenhouse gases, the implementation of stringent standards worldwide for waste discharge into the environment became required. Enzymatic (pre)-treatment systems for solid and liquid effluent is a good alternative to naturally transform and clean environmental waste. Different enzymes from plants, animals, and microorganisms have been reported to play an important role in waste treatment applications. They are used for many biotechnological applications as biocatalysts to remove recalcitrant pollutants, to convert organic matter to high value‐added products, or to render it more amenable.

The worldwide growth of the enzymatic bioindustry reflects also the potential of enzymology in environmental and biotechnology fields. It is in this perspective that the journal Processes is inviting authors to submit original papers, review articles and short communications for the consideration of Special Issue “Applied Enzymology & Environmental Biotechnology”.

Dr. Habib Horchani

Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Processes is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • enzymes
  • biotransformation
  • industrial biotechnology
  • waste management
  • environmental

Published Papers (5 papers)

Download All Papers
Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

20 pages, 3397 KiB  
Article
A Pilot Model for the Treatment of Slaughterhouse Wastewater Using Zeolite or Psidium-Leaf Powder as a Natural Coagulant, Followed by Filtration with Rice Straw, in Comparison with an Inorganic Coagulant
by Fatma Abouelenien, Yossra Ahmed Trabik, Mustafa Shukry, Mohamed El-Sharnouby, Samy Sayed, Ahmed Gaber and Nagham Rafeek Elsaidy
Processes 2022, 10(5), 887; https://doi.org/10.3390/pr10050887 - 29 Apr 2022
Cited by 3 | Viewed by 2174
Abstract
Slaughterhouse wastewater (SHWW) is classified as industrial waste, which is exceptionally harmful to the environment due to its high content of biological oxygen demand (BOD), chemical oxygen demand (COD), and suspended solids, which result from high organic and nutrient loading. This study used [...] Read more.
Slaughterhouse wastewater (SHWW) is classified as industrial waste, which is exceptionally harmful to the environment due to its high content of biological oxygen demand (BOD), chemical oxygen demand (COD), and suspended solids, which result from high organic and nutrient loading. This study used a pilot system to treat SHWW from the Kafrelsheikh Governorate slaughterhouse, which includes a three-step process. It started with sedimentation, then coagulation and flocculation using different concentrations of each: natural zeolites (Z) and Psidium guajava-leaf powder (GLP) as green and environmentally friendly agents, and alum (A) as an inorganic coagulant. The final step was filtration with physically treated rice straw (RS). Each step was judged separately by measuring the removal percentages of each analyzed pollutant, and finally, the overall process was evaluated using the same method. A jar test was used to determine the best concentration of each coagulant used. The measured pollutants were physico-chemical, such as COD, BOD, TSS, TKN, and turbidity. The bacteriological examination included TBC, TCC, and FC. The jar-test results determined that Z 1200 mg/L SHWW, GLP 1 g/L, and A 6 g/L were the best concentrations for each coagulant used. In the coagulation step, GLP 1 g/L gave the highest removal percentage of TSS, TKN, EC, and turbidity, while Z 1200 mg/L gave the highest removal percentage of COD, TDS, TBC, and TCC. From these results, it was concluded that a natural coagulant performs better than a chemical one. Finally, judging the overall pilot test system after applying the filtration with physically treated RS, we found that the best removal efficiencies were obtained from Z 1200 mg/L combined with RS. This combination resulted in 90.58, 83.47, 88.75, 54.89, 21.39, 34.49, 84.16, 99.98, and 99.93 removal percentages for BOD, COD, TSS, TKN, EC, turbidity, TBC, and TCC, respectively. Full article
(This article belongs to the Special Issue Applied Enzymology & Environmental Biotechnology)
►▼ Show Figures

Figure 1

18 pages, 10979 KiB  
Article
Poultry Slaughterhouse Wastewater Remediation Using a Bio-Delipidation Pre-Treatment Unit Coupled with an Expanded Granular Sludge Bed Reactor
by Cebisa Thabo Mdladla, Phumeza Akhona Dyosile, Mahomet Njoya, Moses Basitere, Seteno Karabo Obed Ntwampe and Ephraim Kaskote
Processes 2021, 9(11), 1938; https://doi.org/10.3390/pr9111938 - 29 Oct 2021
Cited by 3 | Viewed by 1860
Abstract
The treatment of poultry slaughterhouse wastewater (PSW) with an Expanded Granular Sludge-Bed Bioreactor (EGSB) is hindered by the washout of activated sludge, and difficulties associated with the operation of the three-phase separator and the determination of the optimum up-flow velocity for sludge-bed fluidization. [...] Read more.
The treatment of poultry slaughterhouse wastewater (PSW) with an Expanded Granular Sludge-Bed Bioreactor (EGSB) is hindered by the washout of activated sludge, and difficulties associated with the operation of the three-phase separator and the determination of the optimum up-flow velocity for sludge-bed fluidization. This results in a poor reactor functionality, and thus a poor performance due to pollutants such as fats, oil and grease (FOG) in the PSW being treated. Hydrolyzing the FOG content with a bio-delipidation, enzyme-based agent in a pre-treatment unit would significantly improve the effectiveness of the primary PSW treating system, i.e., the EGSB. In this study, PSW was pre-treated for 48 h with a biological mixture containing bioflocculants and bio-delipidation constituents. The pre-treated PSW was further treated in an EGSB. The PSW FOG, total chemical oxygen demand (tCOD) and total suspended solids (TSS) content were determined to assess the effectiveness of the pre-treatment process as well as to observe the remedial action of the combined pre-treatment-EGSB system. An increased treatment efficacy was noted for the combined PSW treatment system, whereby the tCOD, FOG and TSS removal averaged 76%, 88% and 87%, respectively. The process developed is intended for micro, small and medium poultry slaughterhouses. Full article
(This article belongs to the Special Issue Applied Enzymology & Environmental Biotechnology)
►▼ Show Figures

Figure 1

15 pages, 1522 KiB  
Article
Palm Oil Decanter Cake Wastes as Alternative Nutrient Sources and Biomass Support Particles for Production of Fungal Whole-Cell Lipase and Application as Low-Cost Biocatalyst for Biodiesel Production
by Aran H-Kittikun, Benjamas Cheirsilp, Natthapat Sohsomboon, Darun Binmarn, Wasu Pathom-aree and Sirasit Srinuanpan
Processes 2021, 9(8), 1365; https://doi.org/10.3390/pr9081365 - 4 Aug 2021
Cited by 6 | Viewed by 3646
Abstract
This is the first report on the possible use of decanter cake waste (DCW) from palm oil industry as alternative nutrient sources and biomass support particles for whole-cell lipase production under solid-state fermentation (SSF) by newly isolated fungal Aspergillus sp. MS15 and their [...] Read more.
This is the first report on the possible use of decanter cake waste (DCW) from palm oil industry as alternative nutrient sources and biomass support particles for whole-cell lipase production under solid-state fermentation (SSF) by newly isolated fungal Aspergillus sp. MS15 and their application as a low-cost and environment-friendly biocatalyst for biodiesel production. The results found that DCW supplemented with 0.1% K2HPO4, 0.05% MgSO4·7H2O, 1% peptone and 2% urea and pH adjusted to 6.0 was optimal for whole-cell lipase production. The optimal moisture content and fermentation temperature was 60% and 37.5 °C, respectively. Environmentally friendly biodiesel production, through either esterification or transesterification using whole-cell lipase immobilized on DCW as a biocatalyst, was optimized. The optimal reaction temperature for both reactions was 37 °C. The whole-cell lipase effectively esterified oleic acid into >95% biodiesel yield through esterification under optimal water activity at 0.71 and an optimal methanol to oleic acid molar ratio of 2:1, and also effectively transesterified palm oil under optimal water activity at 0.81 and an optimal methanol to oil molar ratio of 3:1. The fuel properties of produced biodiesel are close to the international biodiesel standards. These results have shown the circular utilization of palm oil mill waste for the low-cost production of an effective biocatalyst, and may contribute greatly to the sustainability of renewable bioenergy production. Full article
(This article belongs to the Special Issue Applied Enzymology & Environmental Biotechnology)
►▼ Show Figures

Figure 1

13 pages, 2733 KiB  
Article
Alpha Amylase from Bacillus pacificus Associated with Brown Algae Turbinaria ornata: Cultural Conditions, Purification, and Biochemical Characterization
by Mona Alonazi, Aida Karray, Ahmed Yacine Badjah-Hadj-Ahmed and Abir Ben Bacha
Processes 2021, 9(1), 16; https://doi.org/10.3390/pr9010016 - 23 Dec 2020
Cited by 9 | Viewed by 3720
Abstract
We aimed in the current study, the identification of a marine bacterial amylase produced by Bacillus pacificus, which was associated with Turbinaria ornata. Cultural conditions were optimized for the highest amylase production on Tryptic soy broth media supplemented with starch 1% [...] Read more.
We aimed in the current study, the identification of a marine bacterial amylase produced by Bacillus pacificus, which was associated with Turbinaria ornata. Cultural conditions were optimized for the highest amylase production on Tryptic soy broth media supplemented with starch 1% at initial pH 9, 55 °C for 24 h. The newly purified amylase was characterized for a possible biotechnological application. Data indicated that the obtained amylase with a molecular weight of 40 kD and the N-terminal sequence of the first 30 amino acids of amBp showed a high degree of homology with known alpha amylase, and was stable at 60 °C of pH 11. Among the tested substrate analogs, amBp was almost fully active on Alylose and Alylopectine (97%), but moderately hydrolyzed glycogen < sucrose < maltose < lactose. Therefore, the current amylase mainly generated maltohexaose from starch. Mg2+ and Zn2+ improved amylase activity up to 170%. While ethylenediamine tetraacetic acid (EDTA) similarly induced the greatest activity with purified amylase, PCMB had the least effect. Regarding all these characteristics, amylase from marine bacterial symbionts amBp has a new promising feature for probable therapeutic, industrial, and nutritional applications. Full article
(This article belongs to the Special Issue Applied Enzymology & Environmental Biotechnology)
►▼ Show Figures

Figure 1

19 pages, 1927 KiB  
Article
A New Group II Phospholipase A2 from Walterinnesia aegyptia Venom with Antimicrobial, Antifungal, and Cytotoxic Potential
by Islem Abid, Ikram Jemel, Mona Alonazi and Abir Ben Bacha
Processes 2020, 8(12), 1560; https://doi.org/10.3390/pr8121560 - 27 Nov 2020
Cited by 8 | Viewed by 2429
Abstract
Many venomous species, especially snakes, contain a variety of secreted phospholipases A2 that contribute to venom toxicity and prey digestion. We characterized a novel highly toxic phospholipase A2 of group II, WaPLA2-II, from the snake venom of Saudi Walterinnesia aegyptia [...] Read more.
Many venomous species, especially snakes, contain a variety of secreted phospholipases A2 that contribute to venom toxicity and prey digestion. We characterized a novel highly toxic phospholipase A2 of group II, WaPLA2-II, from the snake venom of Saudi Walterinnesia aegyptia (W. aegyptia). The enzyme was purified using a reverse phase C18 column. It is a monomeric protein with a molecular weight of approximately 14 kDa and an NH2-terminal amino acid sequence exhibiting similarity to the PLA2 group II enzymes. WaPLA2-II, which contains 2.5% (w/w) glycosylation, reached a maximal specific activity of 1250 U/mg at pH 9.5 and 55 °C in the presence of Ca2+ and bile salts. WaPLA2-II was also highly stable over a large pH and temperature range. A strong correlation between antimicrobial and indirect hemolytic activities of WaPLA2 was observed. Additionally, WaPLA2-II was found to be significantly cytotoxic only on cancerous cells. However, chemical modification with para-Bromophenacyl bromide (p-BPB) inhibited WaPLA2-II enzymatic activity without affecting its antitumor effect, suggesting the presence of a separate ‘pharmacological site’ in snake venom phospholipase A2 via its receptor binding affinity. This enzyme is a candidate for applications including the treatment of phospholipid-rich industrial effluents and for the food production industry. Furthermore, it may represent a new therapeutic lead molecule for treating cancer and microbial infections. Full article
(This article belongs to the Special Issue Applied Enzymology & Environmental Biotechnology)
►▼ Show Figures

Figure 1

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-5
Back to TopTop