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Processes
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Design of Biocatalytic System in Bioprocess Engineering
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Design of Biocatalytic System in Bioprocess Engineering

A special issue of Processes (ISSN 2227-9717). This special issue belongs to the section "Biological Processes and Systems".

Deadline for manuscript submissions: 31 May 2024 | Viewed by 1806

Special Issue Editors

Dr. Hui Li

E-Mail Website
Guest Editor
College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211816 China
Interests: biocatalysis engineering; bioreaction engineering; bioseparation engineering; process intensification
Dr. Yunting Liu

E-Mail Website
Guest Editor
School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, China
Interests: biocatalysis; enzyme immobilization; cascade catalysis; asymmetric synthesis

Special Issue Information

Dear Colleagues,

Green bio-manufacturing will produce huge economic benefits for contributing to the development of the global bio-economy. The design of the biocatalytic system in bioprocess engineering is a critical initial step in green bio-manufacturing for the synthesis of chemical products, medical products, natural products, and flavor food. Feedstock, reaction medium, and biocatalysts are three main aspects that affect the reaction efficiency of the biocatalytic system. Therefore, its design needs to systematically the roles and interactions between these aspects. In order to synthesize high-value products, whole-cell catalysts, multi-enzyme catalysts, nano-biocatalysts, and the immobilization of biocatalysts have shown remarkable application value in the aqueous phase system, organic phase system, ionic liquid system, two-phase system, deep eutectic solvent system, and non-solvent system.

Dr. Hui Li
Dr. Yunting Liu
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Processes is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • novel biocatalysis platforms
  • whole-cell catalysis
  • multi-enzyme catalysis
  • nano-biocatalyst
  • immobilization of biocatalysts
  • non-aqueous biocatalysis
  • biocatalytic synthesis of natural products
  • biocatalytic synthesis of chemical products
  • biocatalytic synthesis of medical and health products
  • application of biocatalysis in food

Published Papers (1 paper)

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Research

8 pages, 1724 KiB  
Communication
Effects of His-Tag Length on the Soluble Expression and Selective Immobilization of D-Amino Acid Oxidase from Trigonopsis variabilis: A Preliminary Study
by Zhipeng Yan, Qinhe Zhu, Li Ma, Guihui Li, Erzheng Su, Jia Zeng, Yongzhong Chen, Er Meng and Senwen Deng
Processes 2023, 11(6), 1588; https://doi.org/10.3390/pr11061588 - 23 May 2023
Cited by 1 | Viewed by 1599
Abstract
His-tags are widely used for the purification of recombinant proteins. High-cost carriers functionalized with nickel ions are commonly required for the selective immobilization of His-tagged enzymes. In this study, His-tags of varying lengths were fused to the N-terminus of D-amino acid oxidase (DAO) [...] Read more.
His-tags are widely used for the purification of recombinant proteins. High-cost carriers functionalized with nickel ions are commonly required for the selective immobilization of His-tagged enzymes. In this study, His-tags of varying lengths were fused to the N-terminus of D-amino acid oxidase (DAO) from Trigonopsis variabilis. The attachment of a His6 tag significantly improved the solubility of the recombinant DAO expressed in Escherichia coli. By modulating the tag lengths, a better balance between cell growth and protein solubility was achieved, resulting in a higher volume activity (His3). Furthermore, the fusion of longer tags (His6 and His9) facilitated the rapid immobilization of DAOs onto a commercial epoxy carrier without metal bearing, resulting in more selective immobilization. In conclusion, the modulation of His-tag length was preliminarily demonstrated as a simple and cost-effective approach to achieve efficient expression, as well as fast and selective immobilization of DAO. Full article
(This article belongs to the Special Issue Design of Biocatalytic System in Bioprocess Engineering)
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