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Plant Cell and Tissue Culture for Basic Research and Practical...
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Plant Cell and Tissue Culture for Basic Research and Practical Use

A special issue of Plants (ISSN 2223-7747).

Deadline for manuscript submissions: closed (31 March 2021) | Viewed by 26432

Special Issue Editors

Prof. Dr. Elżbieta Kuta

E-Mail Website
Guest Editor
Professor emeritus at Department of Plant Cytology and Embryology, Institute of Botany, Faculty of Biology, Jagiellonian University in Cracow, 9 Gronostajowa Str, 30-387 Cracow, Poland
Interests: plant cell and tissue culture; experimental embryology; plant chromosomes; plant morphology and micromorphology; plant antomy; microevolutionary processes; speciation; role of hybridization and polyploidization in plant evolution; genetic diversity of plant populations; phylogeny; heavy metal impacts on plants
Dr. Aneta Słomka

E-Mail Website
Guest Editor
Faculty of Biology, Institute of Botany, Jagiellonian University in Kraków, 9 Gronostajowa St., 30-387 Cracow, Poland
Interests: plant microevolution and speciation at polluted sites; ecotoxicology; plant physiology; anatomy; embryology; cytology; tissue culture; phytoremediation
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

In vitro plant culture is a good model for studying complex biological processes. A single cell, suspended cells, or tissue systems are suitable for researching cell divisions and differentiation, developmental pathways, genome alteration, defence systems, tolerance to biotic and abiotic stresses, and programmed cell death. Innovative techniques allow the easy isolation of single somatic cells (e.g., leaf epidermis, mesophyll, stomata, hairy roots) or protoplasts as well as generative cells (e.g., egg cell, antipodal cells, synergid cells, sperm cells). Modern molecular methods combined with bioinformatics and developing biochemical techniques allow the quantification of single cell transcriptome, epigenome, proteome, and metabolome. In vitro plant cultures are also used for haploid induction via androgenesis or gynogenesis; endangered, medicinal, ornamental, and crop plant micropropagation via organogenesis or somatic embryogenesis, obtaining secondary metabolites; and in experimental embryology (in vitro pollination, pollen germination, fertilization, and ex vivo plant pro-embryo, embryo, and endosperm development). Another research path is using in vitro techniques for seed germination and determining the molecular mechanism of seedling development.

This Special Issue will accept reviews and full or short research papers from a broad scope of interdisciplinary research on in vitro plant cultures, ranging from a single cell model to tissue cultures and experimental embryology. Of particular interest is works on genes and their expression, signal transduction molecules in the processes of differentiation and development, new insights into cell response to biotic and abiotic stress, hormone-regulated processess, biosynthesis of plant metabolites involved in the plant defense system, and pharmacological characterization of secondary metabolites and their action. Works on in vitro culture, cryopreservation, and artificial seeds in plant gene resource conservation are welcome.

Prof. Dr. Elżbieta Kuta
Dr. Aneta Słomka
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • single cell model
  • micropropagation
  • organogenesis
  • somatic embryogenesis
  • haploid induction
  • secondary metabolites
  • cryopreservation
  • protoplasts

Published Papers (4 papers)

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Research

15 pages, 1412 KiB  
Article
Xanthones Production in Gentiana dinarica Beck Hairy Root Cultures Grown in Simple Bioreactors
by Branka Vinterhalter, Nevena Banjac, Dragan Vinterhalter and Dijana Krstić-Milošević
Plants 2021, 10(8), 1610; https://doi.org/10.3390/plants10081610 - 5 Aug 2021
Cited by 4 | Viewed by 2074
Abstract
The hairy root clones of Gentiana dinarica cl-B, cl-D, cl-3, and cl-14 were cultivated in parallel in diverse simple bioreactors, including temporary immersion systems RITA® (TIS RITA®), bubble column bioreactors (BCB), and Erlenmeyer flasks (EF), and evaluated for biomass production [...] Read more.
The hairy root clones of Gentiana dinarica cl-B, cl-D, cl-3, and cl-14 were cultivated in parallel in diverse simple bioreactors, including temporary immersion systems RITA® (TIS RITA®), bubble column bioreactors (BCB), and Erlenmeyer flasks (EF), and evaluated for biomass production and xanthone content. The obtained results showed that TIS RITA® and BCB containing ½ MS medium with 4% sucrose provided equally good growth conditions in which the majority of the clones displayed the higher percentage of dry matter (DM%), and xanthones norswertianin-1-O-primeveroside (nor-1-O-prim) and norswertianin production than those cultivated in EF. Thin and well branched hairy root clone cl-B grown in BCB for 7 weeks was superior regarding all growth parameters tested, including growth index (19.97), dry weight (2.88 g), and DM% (25.70%) compared to all other clones. Cl-B cultured in TIS RITA® contained the highest amount of nor-1-O-prim (56.82 mg per vessel). In BCB with constant aeration, cl-B accumulated the highest norswertianin content reaching 18.08 mg/vessel. The optimized conditions for cultivation of selected G. dinarica hairy root clones in highly aerated TIS RITA® and BCB systems contribute to the development of bioreactor technology designed for the large scale commercial production of xanthones nor-1-O-prim and norswertianin. Full article
(This article belongs to the Special Issue Plant Cell and Tissue Culture for Basic Research and Practical Use)
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11 pages, 6061 KiB  
Article
Effects of Different Growth Media on In Vitro Seedling Development of an Endangered Orchid Species Sedirea japonica
by Jiae An, Pyoung Beom Kim, Hyeong Bin Park, Seongjun Kim, Hwan Joon Park, Chang Woo Lee, Byoung-Doo Lee, Nam Young Kim and Jung Eun Hwang
Plants 2021, 10(6), 1193; https://doi.org/10.3390/plants10061193 - 11 Jun 2021
Cited by 7 | Viewed by 4858
Abstract
Sedirea japonica is becoming endangered, and even extinct, due to habitat destruction and illegal collection, and the development of an optimized artificial propagation system is necessary for its conservation and reintroduction. Thus, the effects of plant growth medium strength (Murashige and Skoog (MS) [...] Read more.
Sedirea japonica is becoming endangered, and even extinct, due to habitat destruction and illegal collection, and the development of an optimized artificial propagation system is necessary for its conservation and reintroduction. Thus, the effects of plant growth medium strength (Murashige and Skoog (MS) and Hyponex media) and the addition of activated charcoal (AC) and organic supplements on seedling growth of S. japonica were investigated through in vitro seed culture. The results showed that seedling growth was higher in half-strength (1/2) media than in full-strength media. After the addition of AC, the highest leaf area (2.14 cm2) was recorded in the seedlings grown in 1/2 Hyponex medium, and after the addition of organic supplements, root development increased regardless of the media type. Among the sixteen suitable media tested at later seedling growth stages, 1/2 MS medium with the addition of 0.6 g·L−1 AC, 30 g·L−1 banana homogenate and 10 g·L−1 apple homogenate was generally effective in fresh weight (6.13 g) and root length (9.59 cm). We demonstrated which organic supplements are preferred for in vitro growth of seedlings developed from S. japonica protocorms by asymbiotic seed culture, which can be used for mass production and conservation of this rare epiphytic orchid. Full article
(This article belongs to the Special Issue Plant Cell and Tissue Culture for Basic Research and Practical Use)
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11 pages, 3972 KiB  
Article
Sex-Linked Molecular Markers Identify Female Lines in Endosperm-Derived Kiwifruit Callus and in Regenerants
by Iwona Chłosta, Dagmara Kwolek, Elwira Sliwinska, Grzegorz Góralski and Marzena Popielarska-Konieczna
Plants 2021, 10(3), 526; https://doi.org/10.3390/plants10030526 - 11 Mar 2021
Cited by 9 | Viewed by 14871
Abstract
This is the first report of molecular markers application for the analysis of endosperm-derived callus and nonaploid kiwifruit (Actinidia chinensis var. deliciosa, formerly: Actinidia deliciosa) plants. As a source of explants, fruits of ‘Hayward’, the most popular cultivar, were used. Additionally, [...] Read more.
This is the first report of molecular markers application for the analysis of endosperm-derived callus and nonaploid kiwifruit (Actinidia chinensis var. deliciosa, formerly: Actinidia deliciosa) plants. As a source of explants, fruits of ‘Hayward’, the most popular cultivar, were used. Additionally, analyses of the nuclear DNA content and sex were conducted on the regenerated plants. Hexaploid seedlings were used as control for the flow cytometric analyses. Most of the plants (about 90%) regenerated via endosperm-derived callus possessed 2C = 9Cx DNA, which confirmed their endosperm origin and nonaploidy. Because Actinidia is a dioecious species, and female plants bearing fruits are desired by breeders, it is crucial to identify the sex of an individual at early stages of development. Analyses were conducted with ex vitro and in vitro samples. Results revealed that specific markers for a Y-chromosome applied at the callus stage allowed us to reliably predict the sex of plants regenerated from it. This is a novel application of sex-linked markers for early selection of female and male callus lines when the sex of the initial explants is still unknown, such as fresh isolated embryos and endosperm. It may have significant importance for breeding kiwifruit programs, which involve tissue culture techniques. Full article
(This article belongs to the Special Issue Plant Cell and Tissue Culture for Basic Research and Practical Use)
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12 pages, 1835 KiB  
Article
Induction of Hairy Roots on Somatic Embryos of Rhizoclones from Typha domingensis Seedlings
by Guadalupe Hernández-Piedra, Violeta Ruiz-Carrera, Alberto J. Sánchez, Alfonso Azpeitia-Morales and Graciano Calva-Calva
Plants 2020, 9(12), 1679; https://doi.org/10.3390/plants9121679 - 1 Dec 2020
Cited by 3 | Viewed by 2340
Abstract
A protocol for the induction of hairy roots on somatic embryos of rhizoclones from Typha domingensis seedlings grown in hydroponic rhizotron systems was established for the first time. Rhizogenesis was induced through the agrotransformation of somatic embryos in oblong and scutellar states of [...] Read more.
A protocol for the induction of hairy roots on somatic embryos of rhizoclones from Typha domingensis seedlings grown in hydroponic rhizotron systems was established for the first time. Rhizogenesis was induced through the agrotransformation of somatic embryos in oblong and scutellar states of development using the K599, LBA9402, and A4 strains of Agrobacterium rhizogenes. The transfection to the embryos was performed by cocultivation of rhizoclones on a Murashige and Skoog mineral medium at 50% strength (MS0.5), in the dark, at 28 ± 2 °C for 72 h. In contrast to nontransformed embryos that did not exhibit any root tissue, transformed embryos presented hairy roots that varied in number, length, and density depending on the bacterial strain, and K599 was the most effective strain. After analysis via optical microscopy, the transformed embryos were collected and transferred to fresh culture media supplemented with 400 mg mL−1 cefotaxime and 10 mg L−1 ascorbic acid. The efficiency of transformation and survival of the oblong and scutellar embryos were similar among the three bacterial strains. The results show that agrotransformation of somatic embryos of rhizoclones from T. domingensis is a novel and viable strategy for the generation of genetic transformants of Typha that have potential applications in bioremediation technologies. Full article
(This article belongs to the Special Issue Plant Cell and Tissue Culture for Basic Research and Practical Use)
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