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Advances in Plant Regeneration Ⅱ
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Advances in Plant Regeneration Ⅱ

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Development and Morphogenesis".

Deadline for manuscript submissions: closed (30 June 2022) | Viewed by 14773

Special Issue Editors

Prof. Dr. José Manuel Pérez Pérez

E-Mail Website
Guest Editor
Instituto de Bioingeniería, Universidad Miguel Hernández de Elche, 03202 Elche, Spain
Interests: adventitious roots; plant micropropagation; organogenesis; hormone crosstalk; epigenetic regulation
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Pilar S Testillano

E-Mail Website
Guest Editor
Pollen Biotechnology of Crop Plants lab., Biological Research Center, CIB-CSIC, 28040 Madrid, Spain
Interests: somatic embryogenesis; microspore embryogenesis; doubled-haploids; pollen development; stress; phytohormones; epigenetics; cell wall remodeling; autophagy; cell death
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

It is well known that some plant cells are able to regenerate new organs after tissue damage or in response to specific stress treatments and/or exogenous hormone applications. Whole plants can be regenerated even from single protoplasts through de novo organogenesis or somatic embryogenesis. Recent findings have improved our understanding about the molecular mechanisms required for cell reprogramming during plant regeneration. Genetic studies also suggest the involvement of epigenetic regulation during de novo organogenesis. However, there are still some unidentified developmental mechanisms in non-model and crop plants that allow this striking plasticity to be maintained. A better understanding of plant regeneration would help us advance in the optimization of tissue culture, with endless applications in plant micropropagation and biotechnology. This Special Issue of Plants will provide additional insights into the physiological and molecular framework of plant regeneration, the evolutionary conservation of some key regulators, and how developmental and environmental constraints influence these regulatory mechanisms.

Prof. Dr. José Manuel Pérez Pérez
Prof. Dr. Pilar S Testillano
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • cellular reprogramming
  • callus formation
  • de novo organogenesis
  • epigenetic reprograming
  • somatic embryogenesis
  • cell totipotency
  • microspore embryogenesis
  • plant cell culture

Related Special Issue

Published Papers (4 papers)

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Research

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18 pages, 3885 KiB  
Article
Effects of Auxin (Indole-3-butyric Acid) on Adventitious Root Formation in Peach-Based Prunus Rootstocks
by María Salud Justamante, Mariem Mhimdi, Marta Molina-Pérez, Alfonso Albacete, María Ángeles Moreno, Inés Mataix and José Manuel Pérez-Pérez
Plants 2022, 11(7), 913; https://doi.org/10.3390/plants11070913 - 29 Mar 2022
Cited by 8 | Viewed by 2756
Abstract
Several Prunus species are among the most important cultivated stone fruits in the Mediterranean region, and there is an urgent need to obtain rootstocks with specific adaptations to challenging environmental conditions. The development of adventitious roots (ARs) is an evolutionary mechanism of high [...] Read more.
Several Prunus species are among the most important cultivated stone fruits in the Mediterranean region, and there is an urgent need to obtain rootstocks with specific adaptations to challenging environmental conditions. The development of adventitious roots (ARs) is an evolutionary mechanism of high relevance for stress tolerance, which has led to the development of environmentally resilient plants. As a first step towards understanding the genetic determinants involved in AR formation in Prunus sp., we evaluated the rooting of hardwood cuttings from five Prunus rootstocks (Adafuel, Adarcias, Cadaman, Garnem, and GF 677) grown in hydroponics. We found that auxin-induced callus and rooting responses were strongly genotype-dependent. To investigate the molecular mechanisms involved in these differential responses, we performed a time-series study of AR formation in two rootstocks with contrasting rooting performance, Garnem and GF 677, by culturing in vitro microcuttings with and without auxin treatment (0.9 mg/L of indole-3-butyric acid [IBA]). Despite showing a similar histological structure, Garnem and GF677 rootstocks displayed dynamic changes in endogenous hormone homeostasis involving metabolites such as indole-3-acetic acid (IAA) conjugated to aspartic acid (IAA-Asp), and these changes could explain the differences observed during rooting. Full article
(This article belongs to the Special Issue Advances in Plant Regeneration Ⅱ)
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20 pages, 3228 KiB  
Article
In Vitro Propagation, Genetic Assessment, and Medium-Term Conservation of the Coastal Endangered Species Tetraclinis articulata (Vahl) Masters (Cupressaceae) from Adult Trees
by Jorge Juan-Vicedo, Francisco Serrano-Martínez, Miriam Cano-Castillo and José Luis Casas
Plants 2022, 11(2), 187; https://doi.org/10.3390/plants11020187 - 11 Jan 2022
Cited by 8 | Viewed by 3181
Abstract
Tetraclinis articulata (Vahl) Masters is an endangered tree growing in coastal and arid environments that is widely exploited by the timber and resin industry, among other applications. In this context, the use of in vitro techniques is highly encouraged for its propagation. We [...] Read more.
Tetraclinis articulata (Vahl) Masters is an endangered tree growing in coastal and arid environments that is widely exploited by the timber and resin industry, among other applications. In this context, the use of in vitro techniques is highly encouraged for its propagation. We present a protocol for micropropagation using twigs from adult trees as a source of explants. The Schenk and Hildebrandt basal medium (SH) supplemented with 30 g L−1 sucrose, 6.5 g L−1 plant agar, 4.0 mg L−1 6-benzyladenine (BA), and 0.05 mg L−1 1-naphthaleneacetic acid (NAA) provided the optimum multiplication rate (90.48 ± 9.52 explants with basal shoots and 2.58 ± 0.29 basal shoots per explant). Application of activated charcoal (AC) or ½ Knop solution in a liquid overlay produced significantly longer shoots. Supplementation of solid media with indole-3-butyric acid (IBA) or NAA gave low rooting percentages (<17%). Addition of 0.9 g L−1 AC improved rooting (40%) but rooting performance was optimal (66.7%) after a pulse treatment consisting of 4 h immersion in liquid SH medium without growth regulators, followed by 8 weeks of cultivation. Rooted microplants were successfully acclimatized (93.33%) in a peat moss and vermiculite mixture (1:1 v/v ratio). The genetic stability of the in vitro regenerated plantlets was confirmed using the randomly amplified polymorphic DNA (RAPD) technique. Explant survival and growth remained higher than 90% after 28 weeks of cold storage at both 4 °C and 10 °C. The protocol presented here allows for largescale T. articulata production and could be applied for both ex situ conservation strategies and industrial purposes. Full article
(This article belongs to the Special Issue Advances in Plant Regeneration Ⅱ)
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16 pages, 4695 KiB  
Article
Embryogenesis of European Radish (Raphanus sativus L. subsp. sativus Convar. Radicula) in Culture of Isolated Microspores In Vitro
by Elena Victorovna Kozar, Elena Alekseevna Domblides and Alexsey Vasilevich Soldatenko
Plants 2021, 10(10), 2117; https://doi.org/10.3390/plants10102117 - 06 Oct 2021
Cited by 3 | Viewed by 1970
Abstract
The European radish is one of the most unresponsive crops in the Brassicaceae family to embryogenesis in in vitro microspore culture. The aim of this work was to study the process of embryogenesis of European radish and its biological features. In this study, [...] Read more.
The European radish is one of the most unresponsive crops in the Brassicaceae family to embryogenesis in in vitro microspore culture. The aim of this work was to study the process of embryogenesis of European radish and its biological features. In this study, the embryogenesis of European radish is described in detail with illustrative data for the first time. For the first time for the entire family Brassicaceae, the following were found: microspores with intact exines with ordered-like divisions; microspores completely free of exines; and a new scheme of suspensors attachment to the apical parts of embryoids. The morphology of double and triple twin embryoids was described, and new patterns of their attachment to each other were discovered. Uneven maturation of European radish embryoids at all stages of embryogenesis was noted. The period of embryoid maturation to the globular stage of development corresponded, in terms of time, to the culture of B. napus, and into the cotyledonary stage of development, maturation was faster and amounted to 17–23 days. The rate of embryoid development with and without suspensors was the same. Full article
(This article belongs to the Special Issue Advances in Plant Regeneration Ⅱ)
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Review

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35 pages, 3955 KiB  
Review
Effect of LED Lighting on Physical Environment and Microenvironment on In Vitro Plant Growth and Morphogenesis: The Need to Standardize Lighting Conditions and Their Description
by Araceli Barceló-Muñoz, Marta Barceló-Muñoz and Alfonso Gago-Calderon
Plants 2022, 11(1), 60; https://doi.org/10.3390/plants11010060 - 25 Dec 2021
Cited by 14 | Viewed by 5908
Abstract
In the last decades, lighting installations in plant tissue culture have generally been renewed or designed based on LED technology. Thanks to this, many different light quality advances are available but, with their massive implementation, the same issue is occurring as in the [...] Read more.
In the last decades, lighting installations in plant tissue culture have generally been renewed or designed based on LED technology. Thanks to this, many different light quality advances are available but, with their massive implementation, the same issue is occurring as in the 1960s with the appearance of the Grolux (Sylvania) fluorescent tubes: there is a lack of a methodological standardization of lighting. This review analyzes the main parameters and variables that must be taken into account in the design of LED-based systems, and how these need to be described and quantified in order to homogenize and standardize the experimental conditions to obtain reproducible and comparable results and conclusions. We have designed an experimental system in which the values of the physical environment and microenvironment conditions and the behavior of plant tissue cultures maintained in cabins illuminated with two lighting designs can be compared. Grolux tubes are compared with a combination of monochromatic LED lamps calibrated to provide a spectral emission, and light irradiance values similar to those generated by the previous discharge lamps, achieving in both cases wide uniformity of radiation conditions on the shelves of the culture cabins. This study can help to understand whether it is possible to use LEDs as one standard lighting source in plant tissue culture without affecting the development of the cultures maintained with the previously regulated protocols in the different laboratories. Finally, the results presented from this caparison indicate how temperature is one of the main factors that is affected by the chosen light source. Full article
(This article belongs to the Special Issue Advances in Plant Regeneration Ⅱ)
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