Malus Biology

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Crop Physiology and Crop Production".

Deadline for manuscript submissions: closed (31 January 2022) | Viewed by 14453

Special Issue Editors


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Guest Editor
College of Horticulture, Northwest Agriculture and Forestry University, Yangling, Shaanxi 712100, China
Interests: fruit quality; physiological and molecular mechanisms and genetic improvement of the synthetic regulation of malonic acid and Vc content; relationship between apple orchard management and fruit quality formation in arid areas and related technology research and development
Special Issues, Collections and Topics in MDPI journals
College of Horticulture, China Agricultural University, Beijing 100193, China
Interests: genetics; natural variation; fruit quality; abiotic stress; long distance mobile RNA; apple

Special Issue Information

Dear Colleagues,

Apple (Malus domestica L. Borkh), with its wide diversity of climatic adaptation, is the most widely planted tree fruit of the temperate zone and one of the most widely cultivated in the world, covering about 5 million hectares and with a production of more than 75 million metric tons. Apple is a representative model plant of a fruit tree in multiple biological processes, such as fruit set and development, flower development, fruit quality, environmental response, postharvest physiology, grafting, thinning, and domestic evolution, which would not be well reflected in, for example, Arabidopsis, rice, tomato, and other model plants. Apple research has undergone great improvements in recent years, in both quantitative and qualitative terms, and this species genome has been released and widely improved.

Over the last few decades, researchers’ attention on this species has grown exponentially, as evidenced by the increasing number of scientific publications, especially dealing with its basic biology. However, there are still open questions and challenges in this fascinating field, such as apple domestic evolution in quality and stress resistance from the wilds, pome fruit development and flesh quality formation, grafting and rootstock scion interaction, bud differentiation and dormancy, and life cycle regulation. On the other hand, in the pos- genomic era, the analysis of gene functions in apple is required for agricultural utilization and molecular breeding.

In this Special Issue, articles (original research papers, reviews, opinions, perspectives and methods) that focus on Malus biology, including germplasm resources, genome, evolution, development, genetics, stress, fruit quality, nutrition, cultivation and postharvest at all levels comprising transcriptome, proteome, metabolome and epigenome studies and studies on biochemistry, physiology, genes, proteins, metabolites, stress biology, fruit development, fruit quality, grafting biology, flower development, and postharvest biology are most welcome.

Prof. Dr. Mingjun Li
Prof. Dr. Ting Wu
Guest Editors

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Keywords

  • Malus
  • apple
  • genetics
  • stress
  • quality
  • development
  • gene function

Published Papers (4 papers)

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Research

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12 pages, 2336 KiB  
Article
MdMADS6 Recruits Histone Deacetylase MdHDA19 to Repress the Expression of the Carotenoid Synthesis-Related Gene MdCCD1 during Fruit Ripening
by Qiqi Li, Ting Wang, Chen Xu, Meishuo Li, Ji Tian, Yi Wang, Xinzhong Zhang, Xuefeng Xu, Zhenhai Han and Ting Wu
Plants 2022, 11(5), 668; https://doi.org/10.3390/plants11050668 - 28 Feb 2022
Cited by 9 | Viewed by 1811
Abstract
Fruit ripening is regulated by epigenetic modifications and transcription factors, which may function independently or as protein complexes. Our previous study showed that the apple histone deacetylase19 (MdHDA19) suppresses fruit ripening through the deacetylation of histones in related genes. Here, a [...] Read more.
Fruit ripening is regulated by epigenetic modifications and transcription factors, which may function independently or as protein complexes. Our previous study showed that the apple histone deacetylase19 (MdHDA19) suppresses fruit ripening through the deacetylation of histones in related genes. Here, a MADS-box transcription factor (MdMADS6) was identified using a yeast two-hybrid (Y2H) assay as a candidate protein that interacts with MdHDA19 during apple fruit ripening. Furthermore, Y2H, bimolecular fluorescence complementation (BiFC) and pull-down assays were used to confirm the interaction between MdHDA19 and MdMADS6. Agrobacterium-mediated transient transformation and yeast one-hybrid assays showed that MdMADS6 promoted carotenoid accumulation in apple fruit by acting on the downstream target genes related to carotenoid biosynthesis. In summary, we conclude that, in the early stages of fruit development, the expression of MdMADS6 was maintained at lower levels, where it interacted with MdHDA19 to form a protein complex that inhibited the expression of the downstream genes. At the late stages of fruit development, active expression of MdMADS6 dissociated the protein complex of MdMADS6 and MdHDA19 and consequently promoted the expression of carotenoid biosynthesis genes as well as carotenoid accumulation. Full article
(This article belongs to the Special Issue Malus Biology)
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17 pages, 2632 KiB  
Article
The Identification of Small RNAs Differentially Expressed in Apple Buds Reveals a Potential Role of the Mir159-MYB Regulatory Module during Dormancy
by Julio Garighan, Etienne Dvorak, Joan Estevan, Karine Loridon, Bruno Huettel, Gautier Sarah, Isabelle Farrera, Julie Leclercq, Priscila Grynberg, Roberto Coiti Togawa, Marcos Mota do Carmo Costa, Evelyne Costes and Fernando Andrés
Plants 2021, 10(12), 2665; https://doi.org/10.3390/plants10122665 - 03 Dec 2021
Cited by 10 | Viewed by 2695
Abstract
Winter dormancy is an adaptative mechanism that temperate and boreal trees have developed to protect their meristems against low temperatures. In apple trees (Malus domestica), cold temperatures induce bud dormancy at the end of summer/beginning of the fall. Apple buds stay [...] Read more.
Winter dormancy is an adaptative mechanism that temperate and boreal trees have developed to protect their meristems against low temperatures. In apple trees (Malus domestica), cold temperatures induce bud dormancy at the end of summer/beginning of the fall. Apple buds stay dormant during winter until they are exposed to a period of cold, after which they can resume growth (budbreak) and initiate flowering in response to warmer temperatures in spring. It is well-known that small RNAs modulate temperature responses in many plant species, but however, how small RNAs are involved in genetic networks of temperature-mediated dormancy control in fruit tree species remains unclear. Here, we have made use of a recently developed ARGONAUTE (AGO)-purification technique to isolate small RNAs from apple buds. A small RNA-seq experiment resulted in the identification of 17 micro RNAs (miRNAs) that change their pattern of expression in apple buds during dormancy. Furthermore, the functional analysis of their predicted target genes suggests a main role of the 17 miRNAs in phenylpropanoid biosynthesis, gene regulation, plant development and growth, and response to stimulus. Finally, we studied the conservation of the Arabidopsis thaliana regulatory miR159-MYB module in apple in the context of the plant hormone abscisic acid homeostasis. Full article
(This article belongs to the Special Issue Malus Biology)
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17 pages, 33169 KiB  
Article
The RNA Directed DNA Methylation (RdDM) Pathway Regulates Anthocyanin Biosynthesis in Crabapple (Malus cv. spp.) Leaves by Methylating the McCOP1 Promoter
by Yifan Xing, Ziyi Xie, Weilei Sun, Yuying Sun, Zhenyun Han, Shiya Zhang, Ji Tian, Jie Zhang and Yuncong Yao
Plants 2021, 10(11), 2466; https://doi.org/10.3390/plants10112466 - 15 Nov 2021
Cited by 1 | Viewed by 2755
Abstract
The synthesis of anthocyanin pigments in plants is known to be regulated by multiple mechanisms, including epigenetic regulation; however, the contribution of the RNA-directed DNA methylation (RdDM) pathway is not well understood. Here, we used bisulfite sequencing and Real Time (RT)-quantitative (q) PCR [...] Read more.
The synthesis of anthocyanin pigments in plants is known to be regulated by multiple mechanisms, including epigenetic regulation; however, the contribution of the RNA-directed DNA methylation (RdDM) pathway is not well understood. Here, we used bisulfite sequencing and Real Time (RT)-quantitative (q) PCR to analyze the methylation level of the promoter of constitutively photomorphogenic 1 (McCOP1) from Malus cv. spp, a gene involved in regulating anthocyanin biosynthesis. The CHH methylation level of the McCOP1 promoter was negatively correlated with McCOP1 RNA expression, and inhibiting DNA methylation caused decreased methylation of the McCOP1 promoter and asymmetric cytosine CHH methylation. We observed that the McCOP1 promoter was a direct target of the RdDM pathway argonaute RISC component 4 (McAGO4) protein, which bound to a McCOP1 promoter GGTTCGG site. Bimolecular fluorescence complementation (BIFC) analysis showed that RNA-directed DNA methylation (McRDM1) interacted with McAGO4 and another RdDM protein, domains rearranged methyltransferase 2 (McDRM2), to regulate the CHH methylation of the McCOP1 promoter. Detection of CHH methylation and COP1 gene expression in the Arabidopsis thalianaatago4, atdrm2 and atrdm1 mutants showed that RDM1 is the effector of the RdDM pathway. This was confirmed by silencing McRDM1 in crabapple leaves or apple fruit, which resulted in a decrease in McCOP1 CHH methylation and an increase in McCOP1 transcript levels, as well as in anthocyanin accumulation. In conclusion, these results show that the RdDM pathway is involved in regulating anthocyanin accumulation through CHH methylation of the McCOP1 promoter. Full article
(This article belongs to the Special Issue Malus Biology)
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Review

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10 pages, 905 KiB  
Review
Recent Advances in Phytohormone Regulation of Apple-Fruit Ripening
by Yinglin Ji and Aide Wang
Plants 2021, 10(10), 2061; https://doi.org/10.3390/plants10102061 - 30 Sep 2021
Cited by 16 | Viewed by 6302
Abstract
Apple (Malus domestica) is, globally, one of the largest fruits in terms of cultivated area and yield. Apple fruit is generally marketed after storage, which is of great significance for regulating the market supply in the off-season of fruit production. Apple-fruit [...] Read more.
Apple (Malus domestica) is, globally, one of the largest fruits in terms of cultivated area and yield. Apple fruit is generally marketed after storage, which is of great significance for regulating the market supply in the off-season of fruit production. Apple-fruit ripening, which culminates in desirable changes in structural and textural properties, is governed by a complex regulatory network. Much is known about ethylene as one of the most important factors promoting apple-fruit ripening. However, the dynamic interplay between phytohormones also plays an important part in apple-fruit ripening. Here, we review and evaluate the complex regulatory network concerning the action of phytohormones during apple-fruit ripening. Interesting future research areas are discussed. Full article
(This article belongs to the Special Issue Malus Biology)
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