Gametic Embryogenesis

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Development and Morphogenesis".

Deadline for manuscript submissions: closed (31 December 2021) | Viewed by 3166

Special Issue Editors


E-Mail Website
Guest Editor
Departamento de Genética y Producción Vegetal. Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas (EEAD-CSIC), Avda Montañana 1005, 50059 Zaragoza, Spain
Interests: gametic embryogenesis; microspore embryogenesis; gynogenesis; cell totipotency; plant regeneration; chromosome doubling; doubled haploid; somatic embryogenesis; plant breeding

E-Mail Website
Guest Editor
Departamento de Genética y Producción Vegetal. Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas (EEAD-CSIC), Avda Montañana 1005, 50059 Zaragoza, Spain
Interests: doubled haploid technology; totipotency; dedifferentiation; developmental reprograming; epigenetics; microspore embryogenesis molecular markers

Special Issue Information

Dear Colleagues, 

Systems based on the modification of the development program of cells or organs offer an enormous potential in plant biotechnology, and provide valuable materials for plant breeding. One of the most fascinating is gametic embryogenesis. In this process, gamete precursor cells (microspores or young bicellular pollen and ovule) are able to modify their developmental fate and adopt an embryogenic pathway in response or not to a stress treatment. Anthers, isolated microspores, ovules, ovaries, or complete flowers are cultured in vitro, resulting in multicellular structures that differentiate into embryos and finally regenerate haploid or doubled haploid (DH) plants. The value of DH plants in plant breeding programmes is unquestionable, since complete homozygosis is obtained in one generation. Furthermore, the use of a DH population increases the phenotypic selection efficiency, shortening the time required for the release of new varieties or inbred lines to 4–6 years. 

In recent years, increased knowledge of the mechanisms that trigger gametic embryogenesis has revealed the existence of networks involving epigenetic, growth regulators, structural, metabolic, and autophagic factors, among others. Based on these findings, alternative strategies to conventional stress treatments have emerged for gametic embryogenesis induction, allowing the establishment of more efficient protocols. However, many questions remain to be resolved regarding the mechanisms of development pattern shift, the determinants of the embryo fate, or the diverse response of species and cultivars. 

In this Special Issue, reviews and original research articles that propose innovative strategies in gametic embryogenesis protocols or that shed light on the molecular mechanisms involved are welcome.

Dr. Ana Castillo
Dr. María-Pilar Vallés
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • gametic embryogenesis
  • microspore embryogenesis
  • gynogenesis
  • cell totipotency
  • cellular reprogramming
  • plant regeneration
  • doubled haploid
  • “omics” tools

Published Papers (1 paper)

Order results
Result details
Select all
Export citation of selected articles as:

Research

17 pages, 3074 KiB  
Article
Dosage-Dependent Gynoecium Development and Gene Expression in Brassica napus-Orychophragmus violaceus Addition Lines
by Bowei Cai, Tai Wang, Wenqin Fu, Arrashid Harun, Xianhong Ge and Zaiyun Li
Plants 2021, 10(9), 1766; https://doi.org/10.3390/plants10091766 - 25 Aug 2021
Cited by 2 | Viewed by 2309
Abstract
Distant hybridization usually leads to female sterility of the hybrid but the mechanism behind this is poorly understood. Complete pistil abortion but normal male fertility was shown by one Brassica napus-Orychophragmus violaceus monosomic alien addition line (MA, AACC + 1 IO, [...] Read more.
Distant hybridization usually leads to female sterility of the hybrid but the mechanism behind this is poorly understood. Complete pistil abortion but normal male fertility was shown by one Brassica napus-Orychophragmus violaceus monosomic alien addition line (MA, AACC + 1 IO, 2n = 39) produced previously. To study the effect of a single O. violaceus chromosome addition on pistil development in different genetic backgrounds, hybrids between the MA and B. carinata (BBCC), B. juncea (AABB), and two synthetic hexaploids (AABBCC) were firstly produced in this study which show complete female sterility. A microspore culture was further performed to produce the haploid monosomic alien addition line (HMA, AC + 1 IO, 2n = 20) and disomic addition line (DA, AACC + 2 IO, 2n = 40) together with haploid (H, AC, 2n = 19) and double haploid (DH, AACC, 2n = 38) plants of B. napus from MA to investigate the dosage effect of the alien O. violaceus chromosome on pistil development and gene expression. Compared to MA, the development of the pistils of DA and HMA was completely or partially recovered, in which the pistils could swell and elongate to a normal shape after open pollination, although no seeds were produced. Comparative RNA-seq analyses revealed that the numbers of the differentially expressed genes (DEGs) were significantly different, dosage-dependent, and consistent with the phenotypic difference in pairwise comparisons of HMA vs. H, DA vs. DH, MA vs. DH, MA vs. DA, and MA vs. HMA. The gene ontology (GO) enrichment analysis of DEGs showed that a number of genes involved in the development of the gynoecium, embryo sac, ovule, and integuments. Particularly, several common DEGs for pistil development shared in HMA vs. H and DA vs. DH showed functions in genotoxic stress response, auxin transport, and signaling and adaxial/abaxial axis specification. The results provided updated information for the molecular mechanisms behind the gynoecium development of B. napus responding to the dosage of alien O. violaceus chromosomes. Full article
(This article belongs to the Special Issue Gametic Embryogenesis)
Show Figures

Figure 1

Back to TopTop