Application of Biotechnology in Crop Improvement

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Crop Physiology and Crop Production".

Deadline for manuscript submissions: 30 July 2024 | Viewed by 1987

Special Issue Editor


E-Mail Website
Guest Editor
Department of Horticultural Science, North Carolina State University, Mountain Horticultural Crops Research, and Extension Center, Mills River, NC 28759, USA
Interests: breeding and development of tomato lines and varieties with broad adaptation, resistance to major diseases, high yield and fruit quality for fresh market by using conventional and molecular approaches; Investigation of genetic control and inheritance of important traits to facilitate the selection process
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Biotechnology plays a crucial role in improving crops by providing techniques that can increase crop yield, enhance resistance to pests and diseases, and make farming more sustainable, in order to feed the world's growing population. Genetic transformation (GT), genome editing (GE), marker-assisted selection (MAS), genomic selection (GS), and other biotechnologies are among the methods employed for crop improvement. GT enables the introduction of specific genes in order to impart favorable traits to crops, such as resistance to pests, herbicide tolerance, enhanced nutritional content, and improved shelf life. The recent advancements in and future potential of genetic transformation for crop improvement are being explored. Gene editing is a precise technique that modifies a plant's DNA to achieve changes that would occur naturally in a faster and more controlled manner. Recent technologies such as CRISPR-Cas9 have revolutionized crop improvement. Combining the aforementioned methods can minimize controversy and maximize the acceptance of genetically modified crops worldwide. The MAS technique is a DNA-based method that allows breeders to quickly and accurately identify desirable traits in plants without waiting for them to mature. This accelerates the traditional breeding process and enhances its precision by enabling the identification of the specific genes responsible for the desirable traits. Therefore, MAS is a valuable tool for breeders when aiming to select plants with desired traits and improve crop productivity. Genomic selection (GS) studies the complete genetic code of plants to forecast their performance. This technique is especially useful for complex traits like yield, which are influenced by multiple genes. By using genomic selection, breeders can identify and select plants that have the optimum genetic potential for the desired traits. Although technology has immense benefits, it also poses ethical, safety, and regulatory challenges that require careful and thoughtful consideration. Experts worldwide will be invited to submit manuscripts to provide our readers with a comprehensive understanding of these crop improvement tools. Biotechnology is continually advancing and possesses the potential to offer solutions to modern agricultural challenges, such as food security and environmental impact reduction.

Dr. Dilip Panthee
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • crop improvement
  • genetic transformation
  • genome editing
  • genome-wide association studies
  • genomic selection
  • marker-assisted selection
  • QTL analysis
  • RNA-seq analysis

Published Papers (2 papers)

Order results
Result details
Select all
Export citation of selected articles as:

Research

13 pages, 3822 KiB  
Article
Agrobacterium Transformation of Tea Plants (Camellia sinensis (L.) KUNTZE): A Small Experiment with Great Prospects
by Anastasia Fizikova, Elena Subcheva, Nikolay Kozlov, Varvara Tvorogova, Lidia Samarina, Ludmila Lutova and Elena Khlestkina
Plants 2024, 13(5), 675; https://doi.org/10.3390/plants13050675 - 28 Feb 2024
Viewed by 830
Abstract
Tea has historically been one of the most popular beverages, and it is currently an economically significant crop cultivated in over 50 countries. The Northwestern Caucasus is one of the northernmost regions for industrial tea cultivation worldwide. The domestication of the tea plant [...] Read more.
Tea has historically been one of the most popular beverages, and it is currently an economically significant crop cultivated in over 50 countries. The Northwestern Caucasus is one of the northernmost regions for industrial tea cultivation worldwide. The domestication of the tea plant in this region took approximately 150 years, during which plantations spreading from the Ozurgeti region in northern Georgia to the southern city of Maykop in Russia. Consequently, tea plantations in the Northern Caucasus can serve as a source of unique genotypes with exceptional cold tolerance. Tea plants are known to be recalcitrant to Agrobacterium-mediated transfection. Research into optimal transfection and regeneration methodologies, as well as the identification of tea varieties with enhanced transformation efficiency, is an advanced strategy for improving tea plant culture. The aim of this study was to search for the optimal Agrobacterium tumefaciens-mediated transfection protocol for the Kolkhida tea variety. As a result of optimizing the transfection medium with potassium phosphate buffer at the stages of pre-inoculation, inoculation and co-cultivation, the restoration of normal morphology and improvement in the attachment of Agrobacterium cells to the surface of tea explants were observed by scanning electron microscopy. And an effective method of high-efficiency Agrobacteria tumefaciens-mediated transfection of the best local tea cultivar, Kolkhida, was demonstrated for the first time. Full article
(This article belongs to the Special Issue Application of Biotechnology in Crop Improvement)
Show Figures

Figure 1

9 pages, 1987 KiB  
Communication
Somatic Embryogenesis and Agrobacterium-Mediated Gene Transfer Procedures in Chilean Temperate Japonica Rice Varieties for Precision Breeding
by Marion Barrera, Blanca Olmedo, Carolina Zúñiga, Mario Cepeda, Felipe Olivares, Ricardo Vergara, Karla Cordero-Lara and Humberto Prieto
Plants 2024, 13(3), 416; https://doi.org/10.3390/plants13030416 - 31 Jan 2024
Viewed by 856
Abstract
Rice (Oryza sativa) varieties are generated through breeding programs focused on local requirements. In Chile, the southernmost rice producer, rice productivity relies on the use and generation of temperate japonica germplasms, which need to be adapted to the intensifying effects of [...] Read more.
Rice (Oryza sativa) varieties are generated through breeding programs focused on local requirements. In Chile, the southernmost rice producer, rice productivity relies on the use and generation of temperate japonica germplasms, which need to be adapted to the intensifying effects of climate change. Advanced biotechnological tools can contribute to these breeding programs; new technologies associated with precision breeding, including gene editing, rely on procedures such as regeneration and gene transfer. In this study, the local rice varieties Platino, Cuarzo, Esmeralda, and Zafiro were evaluated for somatic embryogenesis potential using a process that involved the combined use of auxins and cytokinins. An auxin-based (2,4-D) general medium (2N6) allowed for the induction of embryogenic masses in all the genotypes. After induction, masses required culturing either in N6R (kinetin; Platino) or N6RN (BAP, kinetin, IBA, and 2,4-D; Cuarzo, Esmeralda, and Zafiro) to yield whole plants using regeneration medium (N6F, no hormone). The sprouting rates indicated Platino as the most responsive genotype; for this reason, this variety was evaluated for gene transfer. Fifteen-day-old embryo masses were assayed for Agrobacterium-mediated transformation using the bacterial strain EHA105 harboring pFLC-Myb/HPT/GFP, a modified T-DNA vector harboring a geminivirus-derived replicon. The vector included the green fluorescent protein reporter gene, allowing for continuous traceability. Reporter mRNA was produced as early as 3 d after agroinfiltration, and stable expression of the protein was observed along the complete process. These achievements enable further biotechnological steps in these and other genotypes from our breeding program. Full article
(This article belongs to the Special Issue Application of Biotechnology in Crop Improvement)
Show Figures

Figure 1

Back to TopTop