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The Role of Abscisic Acid (ABA) Machinery in Stress Response
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The Role of Abscisic Acid (ABA) Machinery in Stress Response

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Response to Abiotic Stress and Climate Change".

Deadline for manuscript submissions: 31 October 2024 | Viewed by 4431

Special Issue Editor

Dr. Gastón Alfredo Pizzio

E-Mail Website
Guest Editor
1. Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia (UPV-CSIC), CP46022 Valencia, Spain
2. CIT-RIO NEGRO Sede Atlántica, Universidad Nacional de Río Negro (UNRN-CONICET), Viedma CP8500, Río Negro, Argentina
Interests: ABA; hormones; signaling; plants; animals; biotechnology
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Increasing global temperatures, in tandem with the predicted increase in the frequency of drought and flooding episodes in the coming years, represent a threat to agricultural productivity. This juncture necessitates the incorporation of strategies for mitigating these threats and, consequently, the need to address crop water use efficiency, ensuring food security.

The phytohormone abscisic acid (ABA) is a key regulator of plant water use efficiency and stress physiology. ABA is also a growth regulator under normal conditions, as well as a developmental cue. ABA’s machinery includes components ranging from synthesis to molecular perception, signaling and response. Moreover, knowledge concerning the fine regulation of these ABA machinery components is emerging. This Special Issue of Plants on “The Role of Abscisic Acid (ABA) Machinery in Stress Response” intends to assemble original research articles and review papers regarding the ABA machinery in plants, ranging from synthesis to response. We are convinced that a better understanding of how ABA machinery works at a basic level would provide us an advantageous position for overcoming the negative food productivity effects of climate change.

Main topics:

  • ABA synthesis and transport;
  • ABA inactivation and conjugation;
  • ABA core signaling function and regulation.

Dr. Gastón Alfredo Pizzio
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • ABA synthesis
  • ABA transport
  • ABA inactivation
  • ABA conjugation
  • ABA perception
  • ABA receptor
  • ABA signaling
  • ABA response
  • PYL receptors
  • PP2CA phosphatases
  • SnRK2 kinases

Published Papers (4 papers)

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Research

16 pages, 9211 KiB  
Article
Effects of Exogenous Abscisic Acid on the Physiological and Biochemical Responses of Camellia oleifera Seedlings under Drought Stress
by Dayu Yang, Yongzhong Chen, Rui Wang, Yimin He, Xiaofan Ma, Jiancai Shen, Zhilong He and Hanggui Lai
Plants 2024, 13(2), 225; https://doi.org/10.3390/plants13020225 - 13 Jan 2024
Viewed by 790
Abstract
This study comprehensively investigates the physiological and molecular regulatory mechanisms of Camellia oleifera seedlings under drought stress with a soil moisture content of about 30%, where exogenous abscisic acid (ABA) was applied via foliar spraying at concentrations of 50 µg/L, 100 µg/L, and [...] Read more.
This study comprehensively investigates the physiological and molecular regulatory mechanisms of Camellia oleifera seedlings under drought stress with a soil moisture content of about 30%, where exogenous abscisic acid (ABA) was applied via foliar spraying at concentrations of 50 µg/L, 100 µg/L, and 200 µg/L. The results demonstrated that appropriate concentrations of ABA treatment can regulate the physiological state of the seedlings through multiple pathways, including photosynthesis, oxidative stress response, and osmotic balance, thereby aiding in the restructuring of their drought response strategy. ABA treatment effectively activated the antioxidant system by reducing stomatal conductance and moderately inhibiting the photosynthetic rate, thus alleviating oxidative damage caused by drought stress. Additionally, ABA treatment promoted the synthesis of osmotic regulators such as proline, maintaining cellular turgor stability and enhancing the plant’s drought adaptability. The real-time quantitative PCR results of related genes indicated that ABA treatment enhanced the plant’s response to the ABA signaling pathway and improved disease resistance by regulating the expression of related genes, while also enhancing membrane lipid stability. A comprehensive evaluation using a membership function approach suggested that 50 µg/L ABA treatment may be the most-effective in mitigating drought effects in practical applications, followed by 100 µg/L ABA. The application of 50 µg/L ABA for 7 h induced significant changes in various biochemical parameters, compared to a foliar water spray. Notably, superoxide dismutase activity increased by 17.94%, peroxidase activity by 30.27%, glutathione content by 12.41%, and proline levels by 25.76%. The content of soluble sugars and soluble proteins rose by 14.79% and 87.95%, respectively. Additionally, there was a significant decrease of 31.15% in the malondialdehyde levels. Full article
(This article belongs to the Special Issue The Role of Abscisic Acid (ABA) Machinery in Stress Response)
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16 pages, 3839 KiB  
Article
Antagonistic Regulation of ABA Responses by Duplicated Tandemly Repeated DUF538 Protein Genes in Arabidopsis
by Yingying Li, Wei Wang, Na Zhang, Yuxin Cheng, Saddam Hussain, Yating Wang, Hainan Tian, Hadia Hussain, Rao Lin, Yuan Yuan, Chen Wang, Tianya Wang and Shucai Wang
Plants 2023, 12(16), 2989; https://doi.org/10.3390/plants12162989 - 19 Aug 2023
Viewed by 826
Abstract
The plant hormone ABA (abscisic acid) regulates plant responses to abiotic stresses by regulating the expression of ABA response genes. However, the functions of a large portion of ABA response genes have remained unclear. We report in this study the identification of ASDs [...] Read more.
The plant hormone ABA (abscisic acid) regulates plant responses to abiotic stresses by regulating the expression of ABA response genes. However, the functions of a large portion of ABA response genes have remained unclear. We report in this study the identification of ASDs (ABA-inducible signal peptide-containing DUF538 proteins), a subgroup of DUF538 proteins with a signal peptide, as the regulators of plant responses to ABA in Arabidopsis. ASDs are encoded by four closely related DUF538 genes, with ASD1/ASD2 and ASD3/ASD4 being two pairs of duplicated tandemly repeated genes. The quantitative RT-PCR (qRT-PCR) results showed that the expression levels of ASDs increased significantly in response to ABA as well as NaCl and mannitol treatments, with the exception that the expression level of ASD2 remained largely unchanged in response to NaCl treatment. The results of Arabidopsis protoplast transient transfection assays showed that ASDs were localized on the plasma membrane and in the cytosol and nucleus. When recruited to the promoter of the reporter gene via a fused GD domain, ASDs were able to slightly repress the expression of the co-transfected reporter gene. Seed germination and cotyledon greening assays showed that ABA sensitivity was increased in the transgenic plants that were over-expressing ASD1 or ASD3 but decreased in the transgenic plants that were over-expressing ASD2 or ASD4. On the other hand, ABA sensitivity was increased in the CRISPR/Cas9 gene-edited asd2 single mutants but decreased in the asd3 single mutants. A transcriptome analysis showed that differentially expressed genes in the 35S:ASD2 transgenic plant seedlings were enriched in several different processes, including in plant growth and development, the secondary metabolism, and plant hormone signaling. In summary, our results show that ASDs are ABA response genes and that ASDs are involved in the regulation of plant responses to ABA in Arabidopsis; however, ASD1/ASD3 and ASD2/ASD4 have opposite functions. Full article
(This article belongs to the Special Issue The Role of Abscisic Acid (ABA) Machinery in Stress Response)
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14 pages, 5352 KiB  
Article
Arabidopsis Protein Phosphatase PIA1 Impairs Plant Drought Tolerance by Serving as a Common Negative Regulator in ABA Signaling Pathway
by Yan Huang, Rongqian Yang, Huiling Luo, Yuan Yuan, Zhihong Diao, Junhao Li, Shihe Gong, Guozhi Yu, Huipeng Yao, Huaiyu Zhang and Yi Cai
Plants 2023, 12(14), 2716; https://doi.org/10.3390/plants12142716 - 21 Jul 2023
Viewed by 1044
Abstract
Reversible phosphorylation of proteins is a ubiquitous regulatory mechanism in vivo that can respond to external changes, and plays an extremely important role in cell signal transduction. Protein phosphatase 2C is the largest protein phosphatase family in higher plants. Recently, it has been [...] Read more.
Reversible phosphorylation of proteins is a ubiquitous regulatory mechanism in vivo that can respond to external changes, and plays an extremely important role in cell signal transduction. Protein phosphatase 2C is the largest protein phosphatase family in higher plants. Recently, it has been found that some clade A members can negatively regulate ABA signaling pathways. However, the functions of several subgroups of Arabidopsis PP2C other than clade A have not been reported, and whether other members of the PP2C family also participate in the regulation of ABA signaling pathways remains to be studied. In this study, based on the previous screening and identification work of PP2C involved in the ABA pathway, the clade F member PIA1 encoding a gene of the PP2C family, which was down-regulated after ABA treatment during the screening, was selected as the target. Overexpression of PIA1 significantly down-regulated the expression of ABA marker gene RD29A in Arabidopsis protoplasts, and ABA-responsive elements have been found in the cis-regulatory elements of PIA1 by promoter analysis. When compared to Col-0, transgenic plants overexpressing PIA1 were less sensitive to ABA, whereas pia1 showed the opposite trait in seed germination, root growth, and stomatal opening experiments. Under drought stress, SOD, POD, CAT, and APX activities of PIA1 overexpression lines were lower than Col-0 and pia1, while the content of H2O2 was higher, leading to its lowest survival rate in test plants, which were consistent with the significant inhibition of the expression of ABA-dependent stress-responsive genes RD29B, ABI5, ABF3, and ABF4 in the PIA1 transgenic background after ABA treatment. Using yeast two-hybrid and luciferase complementation assays, PIA1 was found to interact with multiple ABA key signaling elements, including 2 RCARs and 6 SnRK2s. Our results indicate that PIA1 may reduce plant drought tolerance by functioning as a common negative regulator involved in ABA signaling pathway. Full article
(This article belongs to the Special Issue The Role of Abscisic Acid (ABA) Machinery in Stress Response)
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15 pages, 3191 KiB  
Article
AtbZIP62 Acts as a Transcription Repressor to Positively Regulate ABA Responses in Arabidopsis
by Saddam Hussain, Yuxin Cheng, Yingying Li, Wei Wang, Hainan Tian, Na Zhang, Yating Wang, Yuan Yuan, Hadia Hussain, Rao Lin, Chen Wang, Tianya Wang and Shucai Wang
Plants 2022, 11(22), 3037; https://doi.org/10.3390/plants11223037 - 10 Nov 2022
Cited by 3 | Viewed by 1359
Abstract
The basic region/leucine zipper (bZIP) transcription factor AtbZIP62 is involved in the regulation of plant responses to abiotic stresses, including drought and salinity stresses, NO3 transport, and basal defense in Arabidopsis. It is unclear if it plays a role in regulating plant [...] Read more.
The basic region/leucine zipper (bZIP) transcription factor AtbZIP62 is involved in the regulation of plant responses to abiotic stresses, including drought and salinity stresses, NO3 transport, and basal defense in Arabidopsis. It is unclear if it plays a role in regulating plant responses to abscisic acid (ABA), a phytohormone that can regulate plant abiotic stress responses via regulating downstream ABA-responsive genes. Using RT-PCR analysis, we found that the expression level of AtbZIP62 was increased in response to exogenously applied ABA. Protoplast transfection assays show that AtbZIP62 is predominantly localized in the nucleus and functions as a transcription repressor. To examine the roles of AtbZIP62 in regulating ABA responses, we generated transgenic Arabidopsis plants overexpressing AtbZIP62 and created gene-edited atbzip62 mutants using CRISPR/Cas9. We found that in both ABA-regulated seed germination and cotyledon greening assays, the 35S:AtbZIP62 transgenic plants were hypersensitive, whereas atbzip62 mutants were hyposensitive to ABA. To examine the functional mechanisms of AtbZIP62 in regulating ABA responses, we generated Arabidopsis transgenic plants overexpressing 35S:AtbZIP62-GR, and performed transcriptome analysis to identify differentially expressed genes (DEGs) in the presence and absence of DEX, and found that DEGs are highly enriched in processes including response to abiotic stresses and response to ABA. Quantitative RT-PCR results further show that AtbZIP62 may regulate the expression of several ABA-responsive genes, including USP, ABF2, and SnRK2.7. In summary, our results show that AtbZIP62 is an ABA-responsive gene, and AtbZIP62 acts as a transcription repressor to positively regulate ABA responses in Arabidopsis. Full article
(This article belongs to the Special Issue The Role of Abscisic Acid (ABA) Machinery in Stress Response)
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