Advances in Exosomes in Drug Delivery Systems

A special issue of Pharmaceutics (ISSN 1999-4923). This special issue belongs to the section "Drug Delivery and Controlled Release".

Deadline for manuscript submissions: 30 June 2024 | Viewed by 361

Special Issue Editor

*
E-Mail Website
Guest Editor
Center for Drug Evaluation and Research, Food and Drug Administration, Silver Spring, MD 10903, USA
Interests: nanodrug delivery system; nanomaterials; nanomedicine development; cancer therapy; pharmaceutical formulation development and manufacturing
* Dr. Kibria contributed to this issue in his personal capacity. The views expressed are his own and do not necessarily represent the views of the Food and Drug Administration or the United States Government.
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Special Issue Information

Dear Colleagues,

We are pleased to announce a new Special Issue, entitled ‘Advances in Exosomes in Drug Delivery Systems’, in Pharmaceutics. This Special Issue aims to provide its readers with an update on the advancement of exosomes as promising drug delivery systems for therapeutic applications.

Exosomes are membrane-based extracellular vesicles that are naturally produced by the cells. The nano size range of exosomes and their unique properties, such as stability, biocompatibility and low immunogenicity, are key advantages, make them suitable for nanoparticulate drug delivery systems and also enable them to be considered as promising delivery carriers for future clinical use. In this Special Issue, we would like to invite scholars who are currently working on the current state of the art in the application of exosome or exosome-inspired systems for drug delivery to submit high-quality research and review articles.

The topic includes, but is not limited to, the following:  the composition, biogenesis, isolation and biological and clinical applications of exosomes, as well as the methods employed to characterise them. The biopharmaceutical features of exosomes include the drug/macromolecule loading method, the bioengineering or modification of exosomes and the potential application of exosomes in different diseases.

Dr. Golam Kibria
Guest Editor

Manuscript Submission Information

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Keywords

  • exosomes
  • extracellular vesicles
  • biogenesis of exosomes
  • isolation and characterisation
  • engineering of exosomes
  • drug delivery
  • stability/bioavaiability
  • therapeutic application

Published Papers (1 paper)

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Research

17 pages, 8529 KiB  
Article
Mass Production of Rg1-Loaded Small Extracellular Vesicles Using a 3D Bioreactor System for Enhanced Cardioprotective Efficacy of Doxorubicin-Induced Cardiotoxicity
by Yunfeng Di, Shuang Zhao, Huilan Fan, Wei Li, Guangjian Jiang, Yong Wang, Chun Li, Wei Wang and Jingyu Wang
Pharmaceutics 2024, 16(5), 593; https://doi.org/10.3390/pharmaceutics16050593 (registering DOI) - 26 Apr 2024
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Abstract
Background: Small extracellular vesicles (sEVs) obtained from human umbilical cord mesenchymal stromal cells (MSCs) have shown cardioprotective efficacy in doxorubicin-induced cardiotoxicity (DIC). However, their clinical application is limited due to the low yield and high consumption. This study aims to achieve large-scale production [...] Read more.
Background: Small extracellular vesicles (sEVs) obtained from human umbilical cord mesenchymal stromal cells (MSCs) have shown cardioprotective efficacy in doxorubicin-induced cardiotoxicity (DIC). However, their clinical application is limited due to the low yield and high consumption. This study aims to achieve large-scale production of sEVs using a three-dimensional (3D) bioreactor system. In addition, sEVs were developed to deliver Ginsenoside Rg1 (Rg1), a compound derived from traditional Chinese medicine, Ginseng, that has cardioprotective properties but limited bioavailability, to enhance the treatment of DIC. Methods: The 3D bioreactor system with spinner flasks was used to expand human umbilical cord MSCs and collect MSC-conditioned medium. Subsequently, sEVs were isolated from the conditioned medium using differential ultra-centrifugation (dUC). The sEVs were loaded with Ginsenoside Rg1 by electroporation and evaluated for cardioprotective efficacy using Cell Counting Kit-8 (CCK-8) analysis, Annexin V/PI staining and live cell count of H9c2 cells under DIC. Results: Using the 3D bioreactor system with spinner flasks, the expansion of MSCs reached ~600 million, and the production of sEVs was up to 2.2 × 1012 particles in five days with significantly reduced bench work compared to traditional 2D flasks. With the optimized protocol, the Ginsenoside Rg1 loading efficiency of sEVs by electroporation was ~21%, higher than sonication or co-incubation. Moreover, Rg1-loaded sEVs had attenuated DOX-induced cardiotoxicity with reduced apoptosis compared to free Ginsenoside Rg1 or sEVs. Conclusions: The 3D culture system scaled up the production of sEVs, which facilitated the Rg1 delivery and attenuated cardiomyocyte apoptosis, suggesting a potential treatment of DOX-induced cardiotoxicity. Full article
(This article belongs to the Special Issue Advances in Exosomes in Drug Delivery Systems)
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