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Pathogens
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Development and Evaluation of Detection Method for Pathogenic Bacteria from...
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Development and Evaluation of Detection Method for Pathogenic Bacteria from Food

A special issue of Pathogens (ISSN 2076-0817). This special issue belongs to the section "Bacterial Pathogens".

Deadline for manuscript submissions: 31 August 2024 | Viewed by 3724

Special Issue Editors

Dr. Luca Nalbone

E-Mail Website
Guest Editor
Department of Veterinary Science, University of Study of Messina, 98168 Messina, Italy
Interests: food microbiology; foodborne pathogens; antimicrobial resistance; antimicrobial tolerance; antimicrobial persistence
Special Issues, Collections and Topics in MDPI journals
Dr. Filippo Giarratana

E-Mail Website
Guest Editor
Department of Veterinary Science, University of Study of Messina, 98168 Messina, Italy
Interests: food microbiology; foodborne disease; predictive microbiology; natural antimicrobials; food risk assessment
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Nowadays, increasing public attention is paid to food safety which is jeopardized by new global challenges. The growth of the world population and the consequent demand for food will lead to a significant increase in food production which will require extra efforts to ensure high standards of quality and safety. The scientific community is therefore called to find new solutions to ensure adequate control of foods and guarantee their safety. Over the years, microbial detection techniques have evolved frequently compared to conventional methods and, even today, always new and alternative techniques are proposed. The development of increasingly rapid and effective techniques for the detection of pathogenic microorganisms in foods is crucial to guarantee food safety in view of the significant increase in production in the coming next years.

Both original research and review articles are welcomed. Potential topics include, but are not limited to:

  • New techniques for the detection and enumeration of microorganisms in food;
  • Validation of techniques for the detection and enumeration of microorganisms in food;
  • Evaluation and comparison of the effectiveness of conventional techniques for the detection and enumeration of microorganisms in food;

Dr. Luca Nalbone
Dr. Filippo Giarratana
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Pathogens is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • food microbiology
  • detection and enumeration of microorganisms in food
  • microbial detection techniques
  • foodborne bacteria
  • food safety

Published Papers (3 papers)

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Research

8 pages, 248 KiB  
Communication
Verification of a Rapid Analytical Method for the Qualitative Detection of Listeria spp. and Listeria monocytogenes by a Real-Time PCR Assay according to EN UNI ISO 16140-3:2021
by Veronica Bolzon, Michela Bulfoni, Massimo Pesando, Alessandro Nencioni and Emanuele Nencioni
Pathogens 2024, 13(2), 141; https://doi.org/10.3390/pathogens13020141 - 04 Feb 2024
Viewed by 999
Abstract
Microbial contamination and foodborne infections are a significant global public health concern. For this reason, the detection, monitoring, and characterization of pathogens represent a significant challenge in quality control settings. Standard approaches, such as culture methods and biochemical tests, are known to be [...] Read more.
Microbial contamination and foodborne infections are a significant global public health concern. For this reason, the detection, monitoring, and characterization of pathogens represent a significant challenge in quality control settings. Standard approaches, such as culture methods and biochemical tests, are known to be very time-consuming and intensive. Conversely, molecular technologies based on the genomic identification of bacteria are quick and low-cost. Listeria monocytogenes is an opportunistic pathogen and a major concern especially in food industries. It is important to understand and implement multiple quality control measures to control Listeria infection risk and prevent the contamination of products. Standardized detection and confirmation tests such as the API Listeria test, MALDI-TOF MS, and PCR analysis are available. The aim of our work is to provide a specific molecular method, designed according to the EN UNI ISO 16140-3:2021, for the specific detection, monitoring, and characterization of Listeria spp. and Listeria monocytogenes contamination. The verification of this new rapid approach by real-time PCR (qPCR) overcomes the limitations of culture-based techniques, meeting all the verification criteria required by ISO guidelines, including implementation and item confirmation. This system offers a powerful approach to the real-time assessment of food safety, useful for industry self-monitoring and regulatory inspection. Full article
(This article belongs to the Special Issue Development and Evaluation of Detection Method for Pathogenic Bacteria from Food)
13 pages, 2874 KiB  
Article
Development of Multienzyme Isothermal Rapid Amplification (MIRA) Combined with Lateral-Flow Dipstick (LFD) Assay to Detect Species-Specific tlh and Pathogenic trh and tdh Genes of Vibrio parahaemolyticus
by Seong Bin Park and Yan Zhang
Pathogens 2024, 13(1), 57; https://doi.org/10.3390/pathogens13010057 - 06 Jan 2024
Viewed by 1077
Abstract
Vibrio parahaemolyticus causes severe gastroenteritis in humans after consuming contaminated raw or undercooked seafood. A species-specific marker, the thermolabile hemolysin (tlh) gene, and two pathogenic markers, thermostable-related hemolysin (trh) and thermostable-direct hemolysin (tdh) genes, have been used [...] Read more.
Vibrio parahaemolyticus causes severe gastroenteritis in humans after consuming contaminated raw or undercooked seafood. A species-specific marker, the thermolabile hemolysin (tlh) gene, and two pathogenic markers, thermostable-related hemolysin (trh) and thermostable-direct hemolysin (tdh) genes, have been used to identify V. parahaemolyticus and determine its pathogenicity using both PCR and qPCR assays. To enable testing in field conditions with limited resources, this study aimed to develop a simple and rapid method to detect the species-specific (tlh) and pathogenic (trh and tdh) genes of V. parahaemolyticus using multienzyme isothermal rapid amplification (MIRA) combined with a lateral-flow dipstick (LFD). The amplification of the tlh, trh, and tdh genes could be completed within 20 min at temperatures ranging from 30 to 45 °C (p < 0.05). The test yielded positive results for V. parahaemolyticus but produced negative results for nine Vibrio species and eighteen foodborne pathogenic bacterial species. MIRA-LFD could detect 10 fg of DNA and 2 colony-forming units (CFU) of V. parahaemolyticus per reaction, demonstrating a sensitivity level comparable to that of qPCR, which can detect 10 fg of DNA and 2 CFU per reaction. Both MIRA-LFD and qPCR detected seven tlh-positive results from thirty-six oyster samples, whereas one positive result was obtained using the PCR assay. No positive results for the trh and tdh genes were obtained from any oyster samples using MIRA-LFD, PCR, and qPCR. This study suggests that MIRA-LFD is a simple and rapid method to detect species-specific and pathogenic genes of V. parahaemolyticus with high sensitivity. Full article
(This article belongs to the Special Issue Development and Evaluation of Detection Method for Pathogenic Bacteria from Food)
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20 pages, 11145 KiB  
Article
Addressing Current Challenges in Poultry Meat Safety: Development of a Cultivation and Colony Hybridization Approach to Recover Enterotoxigenic Clostridium perfringens from Broiler Chicken Carcasses
by Rosette Kakese Mukosa, Alexandre Thibodeau, John Morris Fairbrother, William Thériault and Marie-Lou Gaucher
Pathogens 2024, 13(1), 30; https://doi.org/10.3390/pathogens13010030 - 28 Dec 2023
Viewed by 1081
Abstract
Enterotoxigenic Clostridium perfringens is one of the main causes of foodborne illness in Canada. The use of a conventional bacterial culture approach to isolate enterotoxigenic C. perfringens from poultry meat is common. This approach is based on the phenotype attributable to a double [...] Read more.
Enterotoxigenic Clostridium perfringens is one of the main causes of foodborne illness in Canada. The use of a conventional bacterial culture approach to isolate enterotoxigenic C. perfringens from poultry meat is common. This approach is based on the phenotype attributable to a double hemolysis phenomenon, whereas few enterotoxigenic strains of C. perfringens produce it, which further complicates the study of the reservoirs of this important pathogen. The objectives of the current study were to validate the ability of a digoxigenin-labeled probe to detect the C. perfringens cpe gene and to validate the use of either a filtration or a direct plating approach, combined with colony hybridization to detect enterotoxigenic C. perfringens. Pure DNA and pure colonies of enterotoxigenic C. perfringens and broiler chicken carcass rinsate samples were subjected to colony hybridization. The results showed that the synthesized DNA probe can detect the cpe gene from both DNA and pure colonies of enterotoxigenic C. perfringens, and from colonies grown from carcass rinsates artificially contaminated with enterotoxigenic C. perfringens. Our study suggests that this isolation method is a promising tool for a better understanding of the epidemiology of this zoonotic pathogen. Full article
(This article belongs to the Special Issue Development and Evaluation of Detection Method for Pathogenic Bacteria from Food)
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