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Advanced Analytical Techniques in Food Analysis

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Cross-Field Chemistry".

Deadline for manuscript submissions: 15 July 2024 | Viewed by 2731

Special Issue Editor

Special Issue Information

Dear Colleagues,

Consumers need healthy, nutritious, traceable, authentic, safe, and environment-friendly foods. The use of validated analytical methods and the storage of this information in a decentralized public book certificate the food quality. The ISO international certifications have identified some traditional analyses to determine food components and toxins. Unfortunately, many have long analysis times and high costs and require expert personnel for their execution. Nowadays, technologies are available that have short analysis times, low management costs, are more environmentally friendly than official techniques, and often are automated. A robust validation procedure is required when non-certified methods are used, or changes are made to official methods. The purpose of validating an analysis method is to prove the adequacy of the procedure in question, document the operator's competence to conduct the work (by verifying the quality parameters obtained through appropriate procedures) and provide sufficient data to define the control limits that are helpful in verifying compliance with the quality parameters during daily work. The validation process guarantees that the sensitivity of the new tests is equivalent to that of the official methods and their application to the matrix of interest.

This Special Issue collects research articles and reviews papers dealing with the possible uses of innovative technologies in food analysis. In particular, the following topics are of interest:

  • The use of spectrophotometric technologies in food analysis;
  • The use of mass spectroscopy in food analysis;
  • The use of NMR in food analysis;
  • Automated apparatus for food analysis;
  • Enzymatic tests for food analyses;
  • PCR in food analysis;
  • The validation process of food methods;
  • The metrological approach in food analysis.

Prof. Dr. Irene Dini
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • spectroscopic methods
  • validation
  • automated analytical methods
  • metrological approach
  • PCR
  • enzymatic methods

Published Papers (2 papers)

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Research

14 pages, 3658 KiB  
Article
Effect of Vitamin D on the Proliferation and Barrier of Atrophic Vaginal Epithelial Cells
by Dandan Li, Tao Zhang, He Yang, Wenlan Yang, Chi Zhang and Guolan Gao
Molecules 2023, 28(18), 6605; https://doi.org/10.3390/molecules28186605 - 13 Sep 2023
Cited by 1 | Viewed by 1316
Abstract
Atrophic vaginitis is very common in postmenopausal women due to declining estrogen levels. Vitamin D plays an important role in promoting epithelial cell proliferation, migration and adhesion. We established a rat model of ovariectomy (OVX) induced atrophic vaginitis with the aim of investigating [...] Read more.
Atrophic vaginitis is very common in postmenopausal women due to declining estrogen levels. Vitamin D plays an important role in promoting epithelial cell proliferation, migration and adhesion. We established a rat model of ovariectomy (OVX) induced atrophic vaginitis with the aim of investigating the effects of Vitamin D supplementation on the vaginal epithelial barrier. The results showed that ovariectomised rats had significantly higher vaginal pH, reduced Lactobacillus, significantly lower uterine and vaginal weights, and lower vaginal epithelial PCNA, occludin, and E-cadherin mRNA expression compared with sham-operated rats. Vitamin D supplementation could reduce the vaginal pH, promote the proliferation and keratinization of vaginal epithelial cells, enhance the expression of PCNA mRNA in vaginal tissues, and improve the vaginal and uterine atrophy. Vitamin D can also increase the expression of E-cadherin and occludin proteins in vaginal tissues, maintain the integrity of the vaginal epithelium, increase the number of Lactobacillus, and reduce pathogenic bacterial infections. In vitro experiments demonstrated that 1,25(OH)2D3 could promote the proliferation and migration of VK2/E6E7 vaginal epithelial cells and increase the expression of E-cadherin protein. In conclusion, we demonstrated that Vitamin D can regulate the expression of vaginal epithelial tight junction proteins, promotes cell proliferation, and improves vaginal atrophy due to estrogen deficiency. Full article
(This article belongs to the Special Issue Advanced Analytical Techniques in Food Analysis)
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12 pages, 609 KiB  
Article
Validation of an Eco-Friendly Automated Method for the Determination of Glucose and Fructose in Wines
by Irene Dini, Dario Tuccillo, Daniele Coppola, Margherita-Gabriella De Biasi, Elena Morelli and Andrea Mancusi
Molecules 2023, 28(14), 5585; https://doi.org/10.3390/molecules28145585 - 22 Jul 2023
Viewed by 993
Abstract
Fermentable sugar dosage helps oenologists to establish a harvest’s moment and control the fermentation process of the musts. The official analyses recommended for their determination are long, laborious, and must be carried out by specialized personnel. On the contrary, instrumental analysis automation limits [...] Read more.
Fermentable sugar dosage helps oenologists to establish a harvest’s moment and control the fermentation process of the musts. The official analyses recommended for their determination are long, laborious, and must be carried out by specialized personnel. On the contrary, instrumental analysis automation limits human errors, increases precision, and reduces the time and cost of the analyses. In the food production sector, to use methods other than those recommended by supranational bodies in official reports, it is necessary to validate the analytical processes to establish the conformity of the results between the new methods and the reference ones. This work validated an automated enzymatic apparatus to determine the sum of glucose and fructose levels in wine samples. The validation was carried out on wine samples (dry red wine, dry white wine, moderately sweet wine, and sweet wine) containing different sugar concentrations by comparing data obtained using the OIV-MA-AS311-02 method performed by a specialized operator (reference method) and the same method performed by an automated apparatus. The difference between the results’ means obtained with the two procedures was significant. Nevertheless, the automated procedure was considered suitable for the intended use since the differences between the averages were lower than the measurement uncertainty at the same concentration, and the repeatability results were better for the automated procedure than the reference method. Full article
(This article belongs to the Special Issue Advanced Analytical Techniques in Food Analysis)
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