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Microorganisms
Special Issues
Synthetic Biology Applied to Environmental Microorganisms
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Synthetic Biology Applied to Environmental Microorganisms

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Environmental Microbiology".

Deadline for manuscript submissions: closed (31 December 2023) | Viewed by 3707

Special Issue Editors

Prof. Dr. María Eugenia Guazzaroni

E-Mail Website
Guest Editor
Department of Biology, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-900, SP, Brazil
Interests: applied microbiology; metagenomics; systems microbiology; synthetic biology
Dr. Vanesa Amarelle

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Assistant Guest Editor
Departamento de Bioquímica y Genómica Microbianas, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay
Interests: function-driven metagenomics; synthetic biology; microbial biotechnology; antarctic microbiology

Special Issue Information

Dear Colleagues,

Environmental microorganisms have enormous functional potential. They are metabolically versatile and naturally adapted to thrive in different conditions, even the harshest ones. They are an interesting niche in which to discover new enzymes, new metabolites, and novel compounds, and they are also an interesting chassis for different applications. Synthetic biology is undoubtedly a useful approach to gain knowledge on environmental microorganisms and to exploit their functional potential.

In this Special Issue of Microorganisms, we invite researchers to contribute their original articles or reviews regarding synthetic biology approaches applied to environmental microorganisms.

Prof. Dr. María Eugenia Guazzaroni
Dr. Vanesa Amarelle
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Published Papers (2 papers)

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Research

17 pages, 2369 KiB  
Article
Synthetic Biology Toolkit for a New Species of Pseudomonas Promissory for Electricity Generation in Microbial Fuel Cells
by Franciene Rabiço, Matheus Pedrino, Julia Pereira Narcizo, Adalgisa Rodrigues de Andrade, Valeria Reginatto and María-Eugenia Guazzaroni
Microorganisms 2023, 11(8), 2044; https://doi.org/10.3390/microorganisms11082044 - 9 Aug 2023
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Abstract
Microbial fuel cells (MFCs) offer sustainable solutions for various biotechnological applications and are a crucial area of research in biotechnology. MFCs can effectively treat various refuse, such as wastewater and biodiesel waste by decomposing organic matter and generating electricity. Certain Pseudomonas species possess [...] Read more.
Microbial fuel cells (MFCs) offer sustainable solutions for various biotechnological applications and are a crucial area of research in biotechnology. MFCs can effectively treat various refuse, such as wastewater and biodiesel waste by decomposing organic matter and generating electricity. Certain Pseudomonas species possess extracellular electron transfer (EET) pathways, enabling them to transfer electrons from organic compounds to the MFC’s anode. Moreover, Pseudomonas species can grow under low-oxygen conditions, which is advantageous considering that the electron transfer process in an MFC typically leads to reduced oxygen levels at the anode. This study focuses on evaluating MFCs inoculated with a new Pseudomonas species grown with 1 g.L−1 glycerol, a common byproduct of biodiesel production. Pseudomonas sp. BJa5 exhibited a maximum power density of 39 mW.m−2. Also, the observed voltammograms and genome analysis indicate the potential production of novel redox mediators by BJa5. Additionally, we investigated the bacterium’s potential as a synthetic biology non-model chassis. Through testing various genetic parts, including constitutive promoters, replication origins and cargos using pSEVA vectors as a scaffold, we assessed the bacterium’s suitability. Overall, our findings offer valuable insights into utilizing Pseudomonas spp. BJa5 as a novel chassis for MFCs. Synthetic biology approaches can further enhance the performance of this bacterium in MFCs, providing avenues for improvement. Full article
(This article belongs to the Special Issue Synthetic Biology Applied to Environmental Microorganisms)
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18 pages, 1789 KiB  
Article
Enhancing 1,3-Propanediol Productivity in the Non-Model Chassis Clostridium beijerinckii through Genetic Manipulation
by Jonatã Bortolucci, María-Eugenia Guazzaroni, Teresa Schoch, Peter Dürre and Valeria Reginatto
Microorganisms 2023, 11(7), 1855; https://doi.org/10.3390/microorganisms11071855 - 22 Jul 2023
Cited by 1 | Viewed by 1490
Abstract
Biotechnological processes at biorefineries are considered one of the most attractive alternatives for valorizing biomasses by converting them into bioproducts, biofuels, and bioenergy. For example, biodiesel can be obtained from oils and grease but generates glycerol as a byproduct. Glycerol recycling has been [...] Read more.
Biotechnological processes at biorefineries are considered one of the most attractive alternatives for valorizing biomasses by converting them into bioproducts, biofuels, and bioenergy. For example, biodiesel can be obtained from oils and grease but generates glycerol as a byproduct. Glycerol recycling has been studied in several bioprocesses, with one of them being its conversion to 1,3-propanediol (1,3-PDO) by Clostridium. Clostridium beijerinckii is particularly interesting because it can produce a range of industrially relevant chemicals, including solvents and organic acids, and it is non-pathogenic. However, while Clostridium species have many potential advantages as chassis for synthetic biology applications, there are significant limitations when considering their use, such as their limited genetic tools, slow growth rate, and oxygen sensitivity. In this work, we carried out the overexpression of the genes involved in the synthesis of 1,3-PDO in C. beijerinckii Br21, which allowed us to increase the 1,3-PDO productivity in this strain. Thus, this study contributed to a better understanding of the metabolic pathways of glycerol conversion to 1,3-PDO by a C. beijerinckii isolate. Also, it made it possible to establish a transformation method of a modular vector in this strain, therefore expanding the limited genetic tools available for this bacterium, which is highly relevant in biotechnological applications. Full article
(This article belongs to the Special Issue Synthetic Biology Applied to Environmental Microorganisms)
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