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Journals
Microorganisms
Special Issues
Advances in Trypanosoma Infection
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Advances in Trypanosoma Infection

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Parasitology".

Deadline for manuscript submissions: 30 April 2024 | Viewed by 2015

Special Issue Editors

Dr. Ulrike Kemmerling

E-Mail Website
Guest Editor
Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago de Chile 8380453, Chile
Interests: Trypanosoma cruzi; congenital Chagas disease; host–pathogen interaction
Special Issues, Collections and Topics in MDPI journals
Dr. Christian Castillo

E-Mail Website
Guest Editor
1. Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago de Chile 8380453, Chile
2. Facultad de Medicina Veterinaria y Agronomía, Universidad de Las Américas, Santiago 7500975, Chile
Interests: Trypanosoma cruzi; congenital Chagas disease; chronic chagasic cardiopathy; host–pathogen interaction
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Chagas Disease, or American Trypanosomiasis, caused by the protozoan parasite Trypanosoma cruzi, is a silent, underdiagnosed, and life-threatening disease. Nevertheless, it constitutes a significant public health issue in Latin America and worldwide.

The probability of acquiring the disease depends on multiple factors: The parasite, the vectors, the mammalian hosts (including the human being), and socio-economical aspects. Particularly, the host-pathogen interaction is a key factor determining the infection probability and disease establishment. Thus, studying those different aspects is essential to understanding the disease's pathogenesis and improving diagnostic and therapeutic tools for Trypanosoma cruzi infection. 

Therefore, we invite our colleagues to submit their contributions in original research articles, review papers, and short communications about the host-Trypanosoma cruzi interactions for this Special Issue in Microorganisms.

Dr. Ulrike Kemmerling
Dr. Christian Castillo
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Trypanosoma cruzi
  • host-parasite interaction
  • chagas disease treatment
  • American trypanosomiasis

Published Papers (2 papers)

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Research

14 pages, 1256 KiB  
Article
Parasite Burden of Trypanosoma cruzi in Whole Blood and Buffy Coat Determined by Real-Time PCR in Individuals with Chronic Chagas Disease
by Daniela Liempi, Inés Zulantay, Nelson M. Varela, Mauricio Canals, Andrés Guevara, Nicolás Poulsen and Werner Apt
Microorganisms 2024, 12(2), 249; https://doi.org/10.3390/microorganisms12020249 - 25 Jan 2024
Viewed by 862
Abstract
The objective of this study was to compare, by qPCR, the circulating blood parasite load of Trypanosoma cruzi in the buffy coat, and in whole blood mixed with boiled and unboiled guanidine hydrochloride-EDTA buffer, of individuals with chronic ChD. The concentration and purity [...] Read more.
The objective of this study was to compare, by qPCR, the circulating blood parasite load of Trypanosoma cruzi in the buffy coat, and in whole blood mixed with boiled and unboiled guanidine hydrochloride-EDTA buffer, of individuals with chronic ChD. The concentration and purity of DNA were evaluated in a Nanodrop Denovix DS-11FX Series Spectrophotometer (DeNovix Inc., Wilmington, NC, USA). The parasite load was determined with the Taqman® qPCR system using a Stratagene Mx3000P thermocycler (Agilent Technologies, Santa Clara, CA, USA) with Cruzi 1 and Cruzi 2 satellite primers. Student’s t-test with Bonferroni correction, Chi-squared (χ2) tests and Spearman’s correlation coefficient were applied. The concentration and purity of DNA were higher in the buffy coat. Parasite DNA was detected and quantifiable in the three types of samples in seven patients, without statistically significant differences in the parasite load obtained. Higher correlations were found between the total DNA concentrations and the parasite loads obtained in the samples of the buffy coat. Full article
(This article belongs to the Special Issue Advances in Trypanosoma Infection)
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13 pages, 916 KiB  
Article
Experimental Hybrids of the Triatoma brasiliensis Species Complex Show Higher Susceptibility to the Trypanosoma cruzi Infection Than Their Parentals
by Nathália Correia, Letícia Paschoaletto, Carolina Reigada, Teresa Cristina Monte Gonçalves, Carlos José de Carvalho Moreira and Jane Costa
Microorganisms 2023, 11(12), 2850; https://doi.org/10.3390/microorganisms11122850 - 24 Nov 2023
Viewed by 761
Abstract
The Triatoma brasiliensis species complex is a monophyletic group encompassing two subspecies and six species. Recently, a hybrid zone of members of this complex was recorded in the state of Pernambuco. Questions concerning the capability of the hybrids to become infected with Trypanosoma [...] Read more.
The Triatoma brasiliensis species complex is a monophyletic group encompassing two subspecies and six species. Recently, a hybrid zone of members of this complex was recorded in the state of Pernambuco. Questions concerning the capability of the hybrids to become infected with Trypanosoma cruzi have been raised. This study aimed to compare the susceptibility of Triatoma b. brasiliensis, Triatoma juazeirensis, and their experimental hybrids to infection with T. cruzi. We infected the parentals and their experimental hybrids (obtained through reciprocal crosses) through artificial feeding with citrated rabbit blood, to which the TcI 0354 strain of T. cruzi had been added. The insects were weighed before and after feeding on the rabbit blood, and then they were dissected on the 10th, 20th, and 30th day after infection. Both the hybrids and the parentals remained infected throughout the experiment. The parasite was mostly found in the epimastigote form. The number of epimastigotes was significantly lower in the stomach and small intestine of T. juazeirensis than in the hybrids or in T. b. brasiliensis. A significantly higher percentage of metacyclic trypomastigotes was detected in the small intestine and rectum of the hybrids. Hybrids demonstrated higher susceptibility to the TcI 0354 strain than their parentals, opening up new avenues to be investigated. Full article
(This article belongs to the Special Issue Advances in Trypanosoma Infection)
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