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Microorganisms
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Biotechnological Application of Photosynthetic Bacteria 2.0
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Biotechnological Application of Photosynthetic Bacteria 2.0

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Microbial Biotechnology".

Deadline for manuscript submissions: closed (31 May 2023) | Viewed by 4139

Special Issue Editor

Prof. Dr. Hitoshi Miyasaka

E-Mail Website
Guest Editor
Department of Applied Life Science, Sojo University, Kumamoto, Japan
Interests: photosynthetic bacteria; microalgae; agriculture; aquaculture
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

This Special Issue is a continuation of our 2021 Special Issue "Biotechnological Application of Photosynthetic Bacteria". https://www.mdpi.com/journal/microorganisms/special_issues/Biotechnological_Photosynthetic.

Photosynthetic bacteria (PSB) have a wide variety of applications in the fields of agriculture, livestock, aquaculture, waste treatment, energy production, biomaterial production, and bioremediation. Biotechnologies of PSB, therefore, have immense potential to contribute to our society with respect to energy and cost saving, food safety, human and animal health, environment protection, etc.

Among various PSB, purple non-sulfur photosynthetic bacteria (PNSB) are the most investigated and also practically applied PSB because of their nonpathogenic, easily culturable, and fast-growing characteristics.

This Special Issue will contribute to the current knowledge in the field and promote the practical application of PBA biotechnology. We invite you to submit contributions on any aspects related to biotechnological applications of PSB. Although the main interests of the present issue are PNSB, studies on biotechnology of any other kinds of anoxygenic photosynthetic bacteria, such as purple sulfur photosynthetic bacteria, are also welcome. However, cyanobacterial biotechnologies are not included in the scope of this Special Issue.

Prof. Dr. Hitoshi Miyasaka
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biotechnological applications
  • agriculture
  • aquaculture
  • livestock
  • probiotics
  • organic waste treatment
  • renewable energy production
  • biomaterial production
  • environmental applications

Published Papers (2 papers)

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22 pages, 1666 KiB  
Article
Effects of LPS from Rhodobacter sphaeroides, a Purple Non-Sulfur Bacterium (PNSB), on the Gene Expression of Rice Root
by Ranko Iwai, Shunta Uchida, Sayaka Yamaguchi, Daiki Nagata, Aoi Koga, Shuhei Hayashi, Shinjiro Yamamoto and Hitoshi Miyasaka
Microorganisms 2023, 11(7), 1676; https://doi.org/10.3390/microorganisms11071676 - 28 Jun 2023
Viewed by 1185
Abstract
The effects of lipopolysaccharide (LPS) from Rhodobacter sphaeroides, a purple non-sulfur bacterium (PNSB), on the gene expression of the root of rice (Oryza sativa) were investigated by next generation sequencing (NGS) RNA-seq analysis. The rice seeds were germinated on agar [...] Read more.
The effects of lipopolysaccharide (LPS) from Rhodobacter sphaeroides, a purple non-sulfur bacterium (PNSB), on the gene expression of the root of rice (Oryza sativa) were investigated by next generation sequencing (NGS) RNA-seq analysis. The rice seeds were germinated on agar plates containing 10 pg/mL of LPS from Rhodobacter sphaeroides NBRC 12203 (type culture). Three days after germination, RNA samples were extracted from the roots and analyzed by RNA-seq. The effects of dead (killed) PNSB cells of R. sphaeroides NBRC 12203T at the concentration of 101 cfu/mL (ca. 50 pg cell dry weight/mL) were also examined. Clean reads of NGS were mapped to rice genome (number of transcript ID: 44785), and differentially expressed genes were analyzed by DEGs. As a result of DEG analysis, 300 and 128 genes, and 86 and 8 genes were significantly up- and down-regulated by LPS and dead cells of PNSB, respectively. The plot of logFC (fold change) values of the up-regulated genes of LPS and PNSB dead cells showed a significant positive relationship (r2 = 0.6333, p < 0.0001), indicating that most of the effects of dead cell were attributed to those of LPS. Many genes related to tolerance against biotic (fungal and bacterial pathogens) and abiotic (cold, drought, and high salinity) stresses were up-regulated, and the most strikingly up-regulated genes were those involved in the jasmonate signaling pathway, and the genes of chalcone synthase isozymes, indicating that PNSB induced defense response against biotic and abiotic stresses via the jasmonate signaling pathway, despite the non-pathogenicity of PNSB. Full article
(This article belongs to the Special Issue Biotechnological Application of Photosynthetic Bacteria 2.0)
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11 pages, 1449 KiB  
Article
Effects of Seed Bio-Priming by Purple Non-Sulfur Bacteria (PNSB) on the Root Development of Rice
by Ranko Iwai, Shunta Uchida, Sayaka Yamaguchi, Fumika Sonoda, Kana Tsunoda, Hiroto Nagata, Daiki Nagata, Aoi Koga, Midori Goto, Taka-aki Maki, Shuhei Hayashi, Shinjiro Yamamoto and Hitoshi Miyasaka
Microorganisms 2022, 10(11), 2197; https://doi.org/10.3390/microorganisms10112197 - 06 Nov 2022
Cited by 7 | Viewed by 2081
Abstract
The effects of seed bio-priming (seed soaking) with purple non-sulfur bacteria (PNSB) on the grain productivity and root development of rice were examined by a field study and laboratory experiments, respectively. Two PNSB strains, Rhodopseudomonas sp. Tsuru2 and Rhodobacter sp. Tsuru3, isolated from [...] Read more.
The effects of seed bio-priming (seed soaking) with purple non-sulfur bacteria (PNSB) on the grain productivity and root development of rice were examined by a field study and laboratory experiments, respectively. Two PNSB strains, Rhodopseudomonas sp. Tsuru2 and Rhodobacter sp. Tsuru3, isolated from the paddy field of the study site were used for seed bio-priming. For seed bio-priming in the field study, the rice seeds were soaked for 1 day in water containing a 1 × 105 colony forming unit (cfu)/mL of PNSB cells, and the rice grain productivities at the harvest time were 420, 462 and 504 kg/are for the control, strain Tsuru2-primed, and strain Tsuru3-primed seeds, respectively. The effects of seed priming on the root development were examined with cell pot cultivation experiments for 2 weeks. The total root length, root surface area, number of tips and forks were evaluated with WinRhizo, an image analysis system, and strains Tsuru2- and Tsuru3-primed seeds showed better root development than the control seeds. The effects of seed priming with the dead (killed) PNSB cells were also examined, and the seed priming with the dead cells was also effective, indicating that the effects were attributed to some cellular components. We expected the lipopolysaccharide (LPS) of PNSB as the effective component of PNSB and found that seed priming with LPS of Rhodobacter sphaeroides NBRC 12203 (type culture) at the concentrations of 5 ng/mL and 50 ng/mL enhanced the root development. Full article
(This article belongs to the Special Issue Biotechnological Application of Photosynthetic Bacteria 2.0)
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