Advances in Metabolic Engineering of Industrial Microorganisms

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Microbial Biotechnology".

Deadline for manuscript submissions: 15 July 2024 | Viewed by 1386

Special Issue Editors

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Guest Editor
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Interests: metabolic engineering; synthetic biology; industrial microbiology

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Guest Editor
Beijing Advanced Innovation Center for Soft Matter Science and Engineering, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
Interests: metabolic engineering; microbial cell factory; synthetic biology; biofuel; genome editing

Special Issue Information

Dear Colleagues,

The field of metabolic engineering has emerged as a cornerstone in the optimization and manipulation of microbial metabolism for the production of valuable compounds, biofuels, and bioproducts. Industrial microorganisms, harnessed for their diverse metabolic capabilities, hold immense potential for driving sustainable bioprocessing and advancing the bio-based economy. This Special Issue aims to highlight the forefront of progress in metabolic engineering strategies applied to industrial microorganisms, showcasing innovative approaches, breakthroughs, and applications that pave the way for more efficient and eco-friendly production processes.

The Special Issue welcomes original research articles, reviews, and perspectives that span a wide spectrum of topics within the realm of the metabolic engineering of industrial microorganisms, including, but not limited to, the following:

  • Rational and synthetic biology approaches for enhancing microbial productivity;
  • Strain improvement and optimization through genetic modification and selection;
  • Directed evolution and adaptive laboratory evolution for enhanced phenotypes;
  • Systems biology-guided metabolic pathway design and optimization;
  • Pathway engineering for the production of biofuels, chemicals, pharmaceuticals, and biomaterials;
  • Novel tools and techniques for fine-tuning metabolic pathways and regulation;
  • Engineering strategies for enhancing strain robustness, stability, and scalability.

We aim to foster interdisciplinary collaboration and catalyze advancements in the metabolic engineering of industrial microorganisms. We encourage researchers and experts to contribute their groundbreaking work to this Special Issue, driving innovation and knowledge dissemination in the dynamic field of metabolic engineering.

Dr. Shuwen Liu
Prof. Dr. Shuobo Shi
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


  • metabolic engineering
  • microbial metabolism
  • industrial microorganisms

Published Papers (1 paper)

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12 pages, 933 KiB  
An In Vitro Study on the Role of Cellulases and Xylanases of Bacillus subtilis in Dairy Cattle Nutrition
by Valeria Bontà, Marco Battelli, Erlinda Rama, Michela Casanova, Lorenzo Pasotti, Gianluca Galassi, Stefania Colombini and Cinzia Calvio
Microorganisms 2024, 12(2), 300; - 30 Jan 2024
Viewed by 874
The administration of Bacilli to dairy cows exerts beneficial effects on dry matter intake, lactation performance, and milk composition, but the rationale behind their efficacy is still poorly understood. In this work, we sought to establish whether cellulases and xylanases, among the enzymes [...] Read more.
The administration of Bacilli to dairy cows exerts beneficial effects on dry matter intake, lactation performance, and milk composition, but the rationale behind their efficacy is still poorly understood. In this work, we sought to establish whether cellulases and xylanases, among the enzymes secreted by B. subtilis, are involved in the positive effect exerted by Bacilli on ruminal performance. We took advantage of two isogenic B. subtilis strains, only differing in the secretion levels of those two enzymes. A multi-factorial study was conducted in which eight feed ingredients were treated in vitro, using ruminal fluid from cannulated cows, with cultures of the two strains conveniently grown in a growth medium based on inexpensive waste. Feed degradability and gas production were assessed. Fiber degradability was 10% higher (p < 0.001) in feeds treated with the enzyme-overexpressing strain than in the untreated control, while the non-overexpressing strain provided a 5% increase. The benefit of the fibrolytic enzymes was maximal for maize silage, the most recalcitrant feed. Gas production also correlated with the amount of enzymes applied (p < 0.05). Our results revealed that B. subtilis cellulases and xylanases effectively contribute to improving forage quality, justifying the use of Bacilli as direct-fed microbials to increase animal productivity. Full article
(This article belongs to the Special Issue Advances in Metabolic Engineering of Industrial Microorganisms)
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