The Importance of Airborne Microorganisms Spreading Locally or across the Globe: The Main Advantage of Culture and Metagenomic Studies

A special issue of Microorganisms (ISSN 2076-2607).

Deadline for manuscript submissions: closed (31 March 2023) | Viewed by 2469

Special Issue Editor


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Guest Editor
Instituto de Ciencias de la Atmósfera y Cambio Climático UNAM, Mexico City, Mexico
Interests: environmental microbiology; internal and external aerobiology; biotic surfaces

Special Issue Information

Dear Colleagues,

There has been increased interest among environmental microbiologists in the heterogeneity of airborne microorganisms that are able to survive in the atmosphere, as well as for how long. The presence of microorganisms in the atmosphere has significant impacts on human health because of their potential roles in atmospheric events such as cloud formation, precipitation, atmospheric chemistry and mobility of virulence and antibiotic resistance factors.

However, the lack of standardized protocols and methodologies, in addition to technical difficulties, such as low density of microorganisms in the air, inefficient retrieval of microorganisms from this continuous fluid and the spatial and temporal variability of a close airborne microbial community composition, has constrained advances in DNA sequencing and bioinformatics-related studies on airborne microorganisms.

Metagenomic studies offer a unique opportunity to examine viral, bacterial and fungal heterogeneity in the air and monitor their spread, including threats from pathogenic microorganisms. Airborne metagenomic studies could also lead to discoveries of novel genes and metabolic pathways relevant to meteorological and industrial applications, environmental bioremediation and biogeochemical cycles.

It is important to mention that the main advantage of culture-dependent studies is the ability to grow microorganisms in bulk and study them at both the molecular and cellular levels.

Dr. Irma Aurora Rosas-Pérez
Guest Editor

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Keywords

  • airborne microorganisms
  • microbial diversity
  • air metagenomic
  • indoor and outdoor environments
  • advantage of culture and/or metagenome
  • metagenomic protocol
  • microbial ecology
  • human health

Published Papers (1 paper)

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Research

20 pages, 4131 KiB  
Article
Comparison of Atmospheric and Lithospheric Culturable Bacterial Communities from Two Dissimilar Active Volcanic Sites, Surtsey Island and Fimmvörðuháls Mountain in Iceland
by Aurélien Daussin, Pauline Vannier, Marine Ménager, Lola Daboussy, Tina Šantl-Temkiv, Charles Cockell and Viggó Þór Marteinsson
Microorganisms 2023, 11(3), 665; https://doi.org/10.3390/microorganisms11030665 - 6 Mar 2023
Cited by 1 | Viewed by 2226
Abstract
Surface microbes are aerosolized into the atmosphere by wind and events such as dust storms and volcanic eruptions. Before they reach their deposition site, they experience stressful atmospheric conditions which preclude the successful dispersal of a large fraction of cells. In this study, [...] Read more.
Surface microbes are aerosolized into the atmosphere by wind and events such as dust storms and volcanic eruptions. Before they reach their deposition site, they experience stressful atmospheric conditions which preclude the successful dispersal of a large fraction of cells. In this study, our objectives were to assess and compare the atmospheric and lithospheric bacterial cultivable diversity of two geographically different Icelandic volcanic sites: the island Surtsey and the Fimmvörðuháls mountain, to predict the origin of the culturable microbes from these sites, and to select airborne candidates for further investigation. Using a combination of MALDI Biotyper analysis and partial 16S rRNA gene sequencing, a total of 1162 strains were identified, belonging to 72 species affiliated to 40 genera with potentially 26 new species. The most prevalent phyla identified were Proteobacteria and Actinobacteria. Statistical analysis showed significant differences between atmospheric and lithospheric microbial communities, with distinct communities in Surtsey’s air. By combining the air mass back trajectories and the analysis of the closest representative species of our isolates, we concluded that 85% of our isolates came from the surrounding environments and only 15% from long distances. The taxonomic proportions of the isolates were reflected by the site’s nature and location. Full article
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